Preparation method of NGF for cobra venom injection

Abstract

The invention relates to a preparation method of NGF for cobra venom injection, wherein crude cobra venom is subjected to gel chromatography twice, gel chromatography I is SP-Sephadex G-25 column chromatography, gel chromatography II is Acta Rose column chromatography, and snake venom added with a buffer solution before gel chromatography I and crude snake venom nerve growth factor liquid after gel chromatography I are subjected to freezing treatment. The cobra venom nerve growth factor provided by the invention is simple and feasible in preparation method, can be used for industrial large-scale production, and is small in activity loss, and the activity loss of the cobra venom NGF is less than 10%; the conventional limitation of protein separation can be broken through, and the crude snake venom liquid with higher initial concentration can also be used for effective separation; the method is high in production efficiency, reasonable in cost and particularly suitable for industrial large-scale production; the yield is high and exceeds 0.6%, and even can reach 0.7% or above.

Description

Technical field [0001] The present invention relates to a method of preparing an ophthaliotoxic nerve growth factor, and is specifically involved in a method of separating purified nerve growth factors from the gobiis of visual chromatosoprosis. Background technique [0002] Nerve Growth Factor, NGF, nerve growth factor is a bioactive polypeptide that grows in a variety of cells and neurons, and develops important regulatory effects. Since 1984, human beings found NGF and won the Nobel Prize in 1986, and NGF has been found to maintain sympathetic nerve and sensory nerve cell growth, promote neuron growth, development, metabolism, and maintain the normal function of the brain and regulate the endocrine and immune system. Aspects play an important role in the treatment of brain injury, senile dementia, meningitis, vascular neuropathic headache, Parkinson syndrome, etc., etc., which is related to nerve injury and neurodegenesis. [0003] Nerve injury has been considered irreversible...

AI Technical Summary

Problems solved by technology

But this method has these following two fatal weaknesses: (1) generally all need more than 100 hours in the gel filtration process of the first step, not only operation time is long, and the long-time contact of albumen and medium makes required The activity of the target protein may be reduced

(2) The gel filtration method used in the first step can only be used for the preparation of a small amount of samples in the laboratory, and it is difficult to scale up to medium pilot and production scale

[0005] It can be said that despite a lot of research in the laboratory, there is still no industrial-grade, high-yield, low-activity loss, and high-efficiency preparation method for snake venom NGF, especially no preparation method for snake venom NGF that can be used for injections.

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