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Preparation method of total saponins of rhizoma cimicifugae

A technology for the separation and purification of total saponins of Cimicifuga, which is applied in the field of separation and purification of total saponins of Cimicifuga, can solve the problems of large consumption of organic solvents, high risk of explosion, and long time, so as to save separation and purification time, reduce the use of organic solvents and residual effect

Active Publication Date: 2016-06-15
SHANDONG LUYE PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional preparation method of Cimicifuga saponins usually needs to go through steps such as organic solvent extraction, degreasing, extraction, macroporous resin separation and purification. high risk of explosion

Method used

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  • Preparation method of total saponins of rhizoma cimicifugae
  • Preparation method of total saponins of rhizoma cimicifugae
  • Preparation method of total saponins of rhizoma cimicifugae

Examples

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Effect test

Embodiment 1

[0033] Crush Cimicifuga into coarse particles, weigh 250g, heat and reflux extraction with 70% ethanol at 70°C for three times, each time with 2000g of ethanol, filter, combine the filtrates, recover ethanol, and vacuum dry at 60°C to obtain 25.7g of crude extract. Disperse the crude extract in n-hexane to degrease 3 times, use 128.5 g of n-hexane each time, filter, and recover n-hexane. Mix 424 g of water, 177.4 g of ethyl acetate, and 0.32 g of sodium lauryl sulfate, and stir to make a coarse emulsion, then add 169.28 g of absolute ethanol to make a microemulsion. The defatted crude extract was dissolved in microemulsion. After dissolving, add 0.4g sodium chloride and 0.4g sodium carbonate to the microemulsion system to break the emulsion, and let stand to separate. The water layer was discarded, the organic layer was concentrated under reduced pressure to recover the solvent, and vacuum-dried at 60° C. to obtain 8.24 g of total saponins of Cimicifuga with a yield of 32.1%....

Embodiment 2

[0035] Crush Cimicifuga into coarse particles, weigh 250g, heat and reflux extraction with 75% ethanol at 80°C for three times, each time with 2500g of ethanol, filter, combine the filtrates, recover ethanol, and vacuum dry at 60°C to obtain 27.9g of crude extract. Disperse the crude extract in n-hexane to degrease 3 times, use 111.6 g of n-hexane each time, filter, and recover n-hexane. 590 g of water, 279 g of ethyl acetate, and 0.6 g of sodium lauryl sulfate were mixed and stirred to make a coarse emulsion, and then 246.4 g of absolute ethanol was added to make a microemulsion. The defatted crude extract was dissolved in microemulsion. After dissolving, add 0.5g sodium chloride and 0.5g sodium carbonate to the microemulsion system to break the emulsion, and let stand to separate. The water layer was discarded, the organic layer was concentrated under reduced pressure to recover the solvent, and vacuum-dried at 60° C. to obtain 9.52 g of total saponins of Cimicifuga with a ...

Embodiment 3

[0037]Crush Cimicifuga into coarse particles, weigh 250g, heat and reflux extraction with 60% ethanol at 70°C for three times, each time with 1250g of ethanol, filter, combine filtrates, recover ethanol, and vacuum dry at 60°C to obtain 25.1g of crude extract. Disperse the crude extract in n-hexane to degrease 3 times, use 150.6 g of n-hexane each time, filter, and recover n-hexane. Mix 452 g of water, 390 g of ethyl acetate, and 5.6 g of poloxamer and stir to make a coarse emulsion, then add 281.9 g of absolute ethanol to make a microemulsion. The defatted crude extract was dissolved in microemulsion. After the dissolution is complete, add 0.75 g of sodium chloride and 0.75 g of sodium bicarbonate to the microemulsion system to break the emulsion, and let stand to separate. The water layer was discarded, the organic layer was concentrated under reduced pressure to recover the solvent, and vacuum-dried at 60° C. to obtain 8.35 g of total saponins of Cimicifuga with a yield of...

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Abstract

The invention provides a method for preparing total saponins of rhizoma cimicifugae through separation and purification by virtue of a micro emulsion-phase interface extraction technique. The method can achieve the purpose of realizing industrial mass production by virtue of the micro emulsion extraction technique, and the method can save time and reduce the use of an organic solvent; and in addition, the clinical efficacy of the total saponins of rhizoma cimicifugae prepared by the method is better than that of total saponins of rhizoma cimicifugae obtained by a conventional separation and purification method.

Description

technical field [0001] The invention relates to a method for preparing active ingredients in natural medicine Cimicifuga, in particular to a method for separating and purifying total saponins of Cimicifuga. Background technique [0002] Cimicifuga is the dry rhizome of Cimicifugahera cleifolia.Kom., Cimicifugadahurica (Turcz.).Maxim. or Cimicifugafoetida L of the Ranunculaceae plant. It is pungent, slightly sweet, and slightly cold. Return lung, spleen, stomach, large intestine channel. It has the functions of expelling rash, clearing away heat and detoxifying, and promoting Yang Qi. For wind-heat headache, toothache, aphtha, sore throat, impermeable measles, spots of impotence, prolapse of the anus, and prolapse of the uterus. Studies have shown that the total saponins in the aerial part of Xing'an Cimicifuga have obvious pharmacological effects, which can be used to treat women's menopausal syndrome and osteoporosis, and have anti-virus, anti-inflammatory, anti-oxidation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71
Inventor 孙启泉张婷婷孟莹徐敬王君莲范艳丽曲静
Owner SHANDONG LUYE PHARMA CO LTD
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