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Method for removing proteins and pigments in ganoderma lucidum crude polysaccharide

A crude polysaccharide and protein technology, which is applied in the field of biological separation and purification, can solve the problems of unfavorable purification of polysaccharide bioactive substances and industrial production, huge consumption of organic solvents, and great injury to operators, so as to shorten the separation and purification time. , Linear amplification is easy, and the effect of reducing production costs

Active Publication Date: 2012-01-25
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there are many domestic and foreign During the purification process, the protein and etc. Usually, different processes are adopted to remove them separately to improve the purity of polysaccharides, but the operation process is complicated and time-consuming, which is very unfavorable to the purification and factory production of polysaccharide biologically active substances
Moreover, traditional deproteinization and decolorization methods mostly use organic solvents to treat crude polysaccharides, which not only consumes a large amount of organic solvents, but also causes serious loss of polysaccharides, high costs, long operation cycles, and environmental pollution during the treatment process, which causes great harm to operators.

Method used

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  • Method for removing proteins and pigments in ganoderma lucidum crude polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Sugar extraction: take 100g of Ganoderma lucidum, crush it, press Ganoderma lucidum, Add distilled water at a volume ratio of 1:15, place in a water bath at 95-100°C for 2 hours, and filter with suction. The filtrate is concentrated under reduced pressure, alcohol precipitation, centrifugation, and the precipitate is vacuum freeze-dried to obtain Ganoderma lucidum crude polysaccharide.

[0029] 2. Ion exchanger pretreatment: Soak the weakly basic anion exchanger A103S (Deqing Piaolaite (China) Co., Ltd.) in 0.5mol / L NaOH aqueous solution for 4 hours, wash with pure water until neutral, and then place it in 0.5 Soak in mol / L hydrochloric acid for 4 hours, wash with pure water until neutral, and finally soak in 0.5 mol / L NaOH aqueous solution for 4 hours, wash with pure water until neutral to obtain the desired anion exchanger.

[0030] 3. Packing: Pack the anion exchanger into a radial flow chromatographic column with a packing volume of 250ml in the radial column ...

Embodiment 2

[0036] Other operation is the same as embodiment 1, difference

[0037] In the extraction step of Ganoderma lucidum polysaccharide: the mass ratio of Ganoderma lucidum and water is 1:30, and the extraction time is 3 hours;

[0038] In the pretreatment step of the ion exchanger: the weakly basic anion exchanger A103S (Deqing Piao Lai Te (China) Co., Ltd.) was soaked in 1mol / L KOH aqueous solution for 2 hours, washed with pure water until neutral, and then placed in 1mol / L KOH solution. Soak in / L hydrochloric acid for 2 hours, wash with pure water until neutral, and finally soak in 1mol / L KOH aqueous solution for 2 hours, wash with pure water until neutral;

[0039] The protein removal rate obtained in the test was 79.65%, the pigment removal rate was 91.23%, and the polysaccharide recovery rate was 87.35%.

Embodiment 3

[0041] Other operations are the same as embodiment 1, the difference is:

[0042] In the ion exchanger pretreatment step: the kind of weakly basic anion exchanger used is A830 (Deqing Piao Wright (China) Co., Ltd.);

[0043] In the sample loading step: make Ganoderma lucidum crude polysaccharide into a 30mg / ml solution, centrifuge to obtain the supernatant, take 50ml of the supernatant and load it into a radial flow chromatography column at a flow rate of 2ml / min;

[0044] The protein removal rate obtained in the test was 74.06%, the pigment removal rate was 82.83%, and the polysaccharide recovery rate was 78.49%.

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Abstract

The invention discloses a method for removing proteins and pigments in ganoderma lucidum crude polysaccharide, which comprises the following steps of: preparing the ganoderma lucidum crude polysaccharide into 10-30mg / m1 aqueous solution; centrifuging the aqueous solution to take a liquid supernatant; sampling the liquid supernatant; adding the liquid supernatant into a radial flow chromatographiccolumn filled with an anion exchanger; then eluting the chromatographic column with pure water to control a sampling flow velocity to 2-10ml / min and an elution flow velocity to 10-50ml / min; collecting an eluent until no saccharide is detected in the eluent; combining the collected eluent; and carrying out post-treatment to obtain a pure product of the ganoderma lucidum polysaccharide. The method has the advantages that: the proteins and the pigments in the ganoderma lucidum crude polysaccharide are removed simultaneously so as to greatly shorten separation and purification time of the polysaccharide, be favorable for reserving the polysaccharide activity maximally and improve the purification efficiency of the ganoderma lucidum crude polysaccharide; organic solvents are not used in an operation process, conditions are mild, subsequent treatment is convenient, and environmental pollution is lightened; and the iron exchange resins have low cost and can be recycled so as to effectively reduce the production cost.

Description

technical field [0001] The invention belongs to the technical field of biological separation and purification, and in particular relates to a method for removing proteins and pigments from ganoderma lucidum crude polysaccharides. Background technique [0002] Fungal polysaccharides are high-molecular polymers composed of more than 10 monosaccharides linked by glycosidic bonds. They are a type of active polysaccharides that can control cell division and differentiation, and regulate cell growth and aging. Fungal polysaccharides are highly regarded by the pharmaceutical and food industries for their multi-faceted biological activities, safety and non-toxic side effects. . Ganoderma lucidum is a kind of medicinal fungus, which has been used as Expensive Chinese medicine is a large basidiomycete with high nutritional and health value. Ganoderma lucidum polysaccharide is one of the main active components of Ganoderma lucidum, and most of the known pharmacological activities ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 邵平孙培龙何晋浙姜慧燕
Owner ZHEJIANG UNIV OF TECH
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