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A kind of method that utilizes apple branch to prepare phloridzin

A technology for apple branches and phlorizin, which is applied in the field of preparing high-purity phloridzin, can solve the problems of low extract purity, cumbersome production process, application limitation and the like, and achieves the advantages of simple product separation, improved extraction rate and improved promotion effect. Effect

Active Publication Date: 2019-07-12
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction and purification techniques of apple phloridin mainly include solvent extraction, resin purification, polyamide column chromatography, etc., but these extraction methods have more or less defects, such as solvent extraction, resin method, etc. Purification and alumina column refining technology can obtain high-purity phlorizin products, but the production process is cumbersome and lossy, and the application of toxic pore-forming agents in the resin production process limits the application of resins in the production of food and food additives. This defect has become an obstacle for the extraction of phlorizin for food and medicine by the macroporous adsorption resin method: the separation of phlorizin by polyamide chromatography column is mainly used for sample preparation for analysis; although the solvent extraction method only uses ethanol in the extraction process , ethyl acetate and other relatively safe solvents, but the selectivity is poor and the purity of the extract is low, and multiple extractions are required to achieve the effect

Method used

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Experimental program
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Effect test

Embodiment 1

[0016] A kind of method utilizing apple branch to prepare high-purity phlorizin, comprises the following steps:

[0017] 1) Naturally shade-dried apple branches were crushed with a plant grinder, passed through a 40-mesh sieve, and 20 g of branch powder was taken, soaked with 2 g of water, mixed with 0.01 g of cellulase, and pretreated at 35 ° C for 60 min.

[0018] 2) put the mixture of apple tree branch powder and cellulase obtained in step 1) into a 300mL extraction kettle, open the supercritical CO2 extraction device, pressurize and statically extract for 45min under the conditions of extraction pressure 30MPa and extraction temperature 40°C, and then reduce Separation by pressure and collection to obtain phlorizin extract, the yield of phlorizin is 3.23%, and the purity is 29.37%.

[0019] 3) Prepare n-hexane-ethyl acetate-methanol-water (1:5:1:5, v / v) solvent system, after static layering, take the upper phase as the stationary phase, the lower phase as the mobile phase,...

Embodiment 2

[0021] A kind of method utilizing apple branch to prepare high-purity phlorizin, comprises the following steps:

[0022] 1) Naturally shade-dried apple branches were crushed with a plant grinder, passed through a 40-mesh sieve, and 20 g of branch powder was taken, soaked in 2.5 g of water, mixed with 0.02 g of cellulase, and pretreated at 30°C for 45 minutes.

[0023] 2) The mixture of apple branch powder and cellulase obtained in step 1) is packed into a 300mL extraction kettle, and the supercritical CO2 extraction device is opened, and the extraction pressure is 25MPa, and the extraction temperature is 45°C. Separation by pressure and collection to obtain phlorizin extract, the yield of phlorizin is 3.04%, and the purity is 28.76%.

[0024] 3) Prepare n-hexane-ethyl acetate-methanol-water (1:5:1:5, v / v) solvent system, after static layering, take the upper phase as the stationary phase, the lower phase as the mobile phase, and the rotor speed 1000rpm. Dissolve the phlorizi...

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Abstract

The invention discloses a method for preparing high-purity phloridzin by virtue of apple tree branches. The method comprises the steps of raw material pretreatment, supercritical CO2-enzyme extraction, centrifugal partition chromatography purification and the like, and thus, the high-purity phloridzin is finally obtained. The method is applicable to preparation of the high-purity phloridzin with the apple tree branches as raw materials. According to the method, a supercritical CO2 extraction technology is combined with an enzyme-assisted extraction technology, and an enzymolysis process is implemented in supercritical CO2, so that enzymolysis effects and promotion effects on phloridzin extraction are greatly improved; in addition, supercritical CO2 extraction is high in selectivity, an entrainer is not required, and the product is easy to separate and high in purity; the extraction rate of the phloridzin is effectively increased, the high-purity phloridzin is obtained, the separation and purification time is saved, the yield is high, preparation procedures are simplified, and the method is a safe and efficient apple phloridzin extraction process, and has broad application prospect.

Description

technical field [0001] The invention relates to the technical field of extracting phloridzin, in particular to a method for preparing high-purity phloridzin from apple branches. Background technique [0002] Phlorizin is a characteristic polyphenol component in apples, which widely exists in apple peel, pulp, seeds, branches and leaves, etc. It has the functions of lowering blood sugar, whitening, antioxidation, enhancing memory, preventing osteoporosis, Various biological activities such as tumors have important uses in new drugs and functional foods. There are many apple orchards in my country, and the apple planting area is large. Every year, a large number of apple branches are produced due to tree pruning and tree species renewal in orchards. These are excellent raw materials for extracting and separating phlorizin. Therefore, the research and development of safe and efficient extraction and purification technology of apple phloridin is of great significance for the ef...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/203C07H1/08
CPCC07H1/08C07H15/203Y02P20/54
Inventor 焦中高杨文博张春岭李荣涛陈大磊刘杰超
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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