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Industria production method for separating and purifying chicken eimeria coccidium oocyst

A technology for separation and purification of chicken emerald balls, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unsuitable industrial production, laborious, and time-consuming oocyst purification technology, and achieve reduction The effect of instability and cost reduction

Inactive Publication Date: 2014-06-25
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

Traditionally used purification techniques for oocysts are time-consuming and labor-intensive, and are not suitable for industrial production. There is an urgent need for technical processes suitable for large-scale production

Method used

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Examples

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Embodiment 1

[0030] An industrialized production method for the purification of Eimeria chicken oocysts, the specific operation is as follows:

[0031] (1) Treatment of feces samples: collect the feces 120 to 192h after the chicken is infected with coccidiosis, add 10 times the volume of double distilled water to resuspend the feces, after soaking for 2h, use the self-flow method to make the fecal mixture use its own pressure difference and pressure Into the disc separator, 3000g / min, 10min, centrifuge to collect the sediment;

[0032] (2) After passing through the disc centrifuge, the supernatant is again passed through a tube centrifuge with a peristaltic pump, and centrifuged at 3000g / min for 10min to collect the precipitate;

[0033] (3) Mix the two centrifugal sediments, add 3 times the volume of saturated saline to the sediments, through the self-flow method, make the fecal mixture use its own pressure difference, press into the disc separator, 2000g / min, 10min, centrifuge and collect Supe...

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PUM

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Abstract

The invention discloses an industrialized production method for separating and purifying a chicken eimeria coccidium oocyst. According to the industrialized production method, the separation and purification of the chicken eimeria coccidium oocyst are carried out by adopting the scientific assembly of a disc separator and a tubular type centrifuge, after sporulated oocysts are purified, larger impurities and extremely tiny impurities are not generated; an object plate is thoroughly subjected to microscopic examination, unsporulated oocysts are also all thoroughly removed, sporulated oocysts are all in the visual field, and the solution background is clear. The industrial production method disclosed by the invention can be used for shortening the separation and purification time of the oocysts; compared with the traditional technology, the industrial production method disclosed by the invention can be used for saving the time by more than 70%, reducing the product instability caused by excessive human factors by preventing integral oocyst purification from the disturbance of excessive artificial working and is the breakthrough of the existing coccidium vaccine production method; the industrial production method can be used for continuous production by only needing two centrifuges, thereby greatly reducing the cost input of an enterprise.

Description

technical field [0001] The invention relates to an industrial production method for separating and purifying Eimeria gallinarum oocysts. Background technique [0002] chicken coccidiosis ( Coccidiosis ) from the genus Eimeria ( Eimeria ) of one or several species of coccidia ( Coccidium ) Simultaneously or successively parasitize the disease caused by chicken intestinal mucosal cells. Coccidiosis occurs wherever chickens are raised in the world, and large-scale chicken farms are suitable places where chicken coccidiosis is prone to occur, and outbreaks of the disease often occur. According to statistics, there are 15 kinds of chicken coccidia reported in the whole world, wherein, 5 kinds have obvious pathogenicity, and they are: Eimeria tenella ( E. tenella ), poisonous Eimeria ( E. necatrix ), Eimeria giga ( E. maxima ), Eimeria hemiformis ( E. acervulina ) and Eimeria placidum ( E.mitis ). Chicks and young chickens are susceptible to coccidiosis, and the mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/10C12R1/90
Inventor 吴彩艳孙铭飞戚南山廖申权吕敏娜李娟谢明权温列娜
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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