32 results about "Coccidulini" patented technology

Disclosed are novel pharmaceutical, animal feed compositions and methods of treating non-human animals comprising at least one component selected from the extract(s), fraction(s), active compound(s) and phytochemical(s), or mixtures thereof, derived from non-woody plants of the genus Hesperaloe. Animal feed compositions may comprise a basal animal feed and water soluble solids extracted from Hesperaloe and comprising at least one saponin. The water soluble solids may comprise from about 5 to about 30 wt % saponin. The compositions of the present invention can be used for the treatment of non-human animals, such as poultry and more particularly for preventing and treating coccidiosis. An embodiment provides an immunological composition useful for inducing the production of antibodies to an antigen in a non-human animal comprising an antigen, preferably a coccidia, and a saponin composition extracted from Hesperaloe. The saponins extracted from Hesperaloe biomass may comprise 25(27)-dehydrofucreastatin, 5(6),25(27)-disdehydroyuccaloiside C, 5(6)-disdehydroyuccaloiside C, furcreastatin and yuccaloiside C.

The present disclosure provides a method of reducing, preventing or treating an intestinal infection, preventing or treating a disease associated with protozoan parasite of the genus Eimeria, inhibiting an oocyst sporulation of a protozoan parasite or preventing a sporozoite invasion or reproduction, preventing or treating coccidiosis, promoting growth performance, controlling, treating and / or preventing necrotic enteritis in a subject, comprising administering to the subject an effective amount of a composition comprising a Crassocephalum rabens (CR), a bioactive extract thereof (CRE), an active ingredient(s) contained in a CR (CR_API) or CRE (CRE_API), a combination of two or more of a CR, a CRE, a CR_API and a CRE_API, or one or more of a-linolenic acid, citric acid, lactic acid, mannitol, palmitic acid, aspartic acid and 1-monomyristin.

The application discloses a phytosterol and phytosterol ester complex agent and application thereof, wherein the complex agent comprises phytosterol and phytosterol ester with a mass ratio of 1:10. The application aims to improve the water solubility and bioavailability of phytosterol, realize synergistic effect by compounding phytosterol and phytosterol ester, interfere with the lipid acquisition of coccidia from multiple targets, up-regulate the expression of cecal tight junction protein to repair the intestinal barrier, inhibit the release of serum and cecal pro-inflammatory factors to relieve inflammatory reaction, regulate the expression of genes related to lipid synthesis and fatty acid oxidation to remodel lipid metabolism homeostasis, and then effectively inhibit the growth and development of coccidia, and significantly improve the growth performance of broilers infected with coccidia.

This invention provides a feed additive and compound feed for preventing and treating coccidiosis in broilers, comprising the following components: *Kluyveromyces martensii*, ferulic acid esterase, aminopeptidase, and glucanase. Preferably, the mass percentage of *Kluyveromyces martensii* is 30-40%; the mass percentage of ferulic acid esterase is 15-28%; the mass percentage of aminopeptidase is 15-25%; and the mass percentage of glucanase is 18-25%. Based on extensive research, this invention has determined that this combination has a significant effect on coccidiosis control in broilers, effectively improving broiler production performance indicators, increasing economic benefits, and reducing the use of anticoccidial drugs.

The application relates to the technical field of veterinary traditional Chinese medicines, in particular to an anticoccidial combined extract, an anticoccidial composite additive and a preparation method and application thereof, and an anticoccidial product. The anticoccidial combined extract contains Terminalia chebula extract and Saussurea costus extract in a specific mass ratio, has a remarkable anticoccidial effect without other components, can significantly inhibit the activity of dihydroorotate dehydrogenase (DHODH), improve an anticoccidial index (ACI), reduce the oocyst production of coccidia, and reduce the degree of intestinal lesions of chickens caused by coccidia; meanwhile, the anticoccidial combined extract can significantly improve the daily weight gain and daily feed intake of broilers, reduce the feed-meat ratio, and has a double effect of “disease prevention + yield increase”.

The invention discloses an eimeria gene editing system, a recombinant coccidium vector and application of the recombinant coccidium vector. The invention firstly provides the sgRNA targeting the ETH00010755 gene and the gene editing system containing the sgRNA, and the sgRNA and the gene editing system can realize high-efficiency and high-specificity gene editing. A donor plasmid template containing His4 and EtU6 promoters is constructed, compared with an original plasmid template, the donor plasmid template has the advantages that the positive rate is higher, an exogenous gene can be accurately inserted into an ETH00010755 gene, the unspecific gene editing risk can be avoided through the gene editing plasmid, and the high editing efficiency and the low off-target rate are achieved. The gene editing system, the recombinant coccidiosis vector and the construction method of the recombinant coccidiosis vector are mature, reliable and easy to operate and have high success rate and repeatability, and the obtained recombinant coccidiosis vector can be applied to preparation of immune drugs and / or vaccines for preventing eimeria.

Avian coccidiosis is a disease caused by an intracellular parasitic protozoan of the genus Eimeria, which induces lesions in the gastrointestinal tissues of birds due to its replication. The process of qualifying an oocyst is almost entirely manual, using, in particular, the optical microscope counting technique, in which each identified object must be classified as sporulated or non-sporulated, stained or unstained. For the identification process, the method used is PCR (polymerase chain reaction), in which the presence of each species is detected, but without identifying each oocyst. This manual process is time-consuming and susceptible to errors. The method proposed herein introduces a computer vision model, based on Artificial Intelligence, capable of automating this classification process. The model analyzes samples and provides quantitative results, including the classification of oocysts into the categories of sporulated, non-sporulated, stained, and unstained, as well as the identification of Eimeria species (acervulina, brunetti, maxima, mitis, necatrix, praecox, or tenella). This computational model is entirely based on machine learning and is capable of identifying the presence of oocysts in a sample (image), classifying each identified oocyst into categories such as sporulated or non-sporulated, stained or unstained, quantifying the objects in each category, and identifying the Eimeria species.

The present disclosure relates to a composition comprising quinic acid for preventing, alleviating or treating coccidiosis, and an antiprotozoal composition against Eimeria sporozoites.

The present application relates to the use of a composition for preventing, alleviating or treating coccidiosis and against Eimeria protozoa. The composition comprises an active ingredient for preventing, relieving or treating coccidiosis, and the active ingredient consists of coumaric acid or a pharmaceutically acceptable salt thereof. According to one embodiment, the composition comprising cumaric acid and / or a salt thereof has an excellent effect of inhibiting cell infiltration of protozoa capable of inducing coccidiosis and / or inhibiting proliferation of protozoa in cells, has an excellent effect of preventing, alleviating and treating coccidiosis in vivo, and significantly reduces the oocyst excretion rate in faeces, and thus has an excellent effect of preventing, alleviating and treating coccidiosis in vivo. Therefore, secondary coccidiosis infection can be reduced.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to drug resistance of Eimeria coccidiosis to diclazuril and application of the SNP molecular marker. The SNP site aiming at the eimeria tenella is selected from at least one of the 574th site, the 1303rd site and the 1394th site of the DNA fragment with the nucleotide sequence of SEQ ID NO: 1; the SNP site aiming at the eimeria heap is selected from at least one of the 556 site, the 1210 site and the 1301 site of the DNA fragment with the nucleotide sequence of SEQ ID NO: 2. The invention provides an SNP molecular marker related to the drug resistance of Eimeria to diclazuril and a primer for detecting the genotype of an SNP site, and is suitable for identification or auxiliary identification of the drug resistance of Eimeria to diclazuril, and the identification result has high accuracy; a reliable basis is provided for screening and detecting diclazuril drug-resistant strains, and scientific prevention and control of coccidiosis and improvement of production benefits of farms are facilitated.

This invention relates to the field of veterinary medicine technology and provides a traditional Chinese medicine composition for treating coccidiosis in chickens, along with its preparation method. The composition is prepared from a combination of raw materials including *Gnaphalium affine*, *Hedyotis diffusa*, *Dendrobium nobile*, *Hemiberlesia argyi*, and *Thalictrum sylvestris*. This pure traditional Chinese medicine preparation exhibits no drug resistance, no drug residues, no toxic side effects, and no palatability issues. It also possesses advantages such as significant efficacy, rational formulation, simple preparation, low cost, and environmental friendliness. The traditional Chinese medicine composition of this invention has the effects of stopping diarrhea and killing parasites, clearing heat and drying dampness, and tonifying the middle and replenishing qi. It has a significant effect against coccidiosis in chickens, effectively preventing abnormal feces, intestinal bleeding, and intestinal ulcers caused by coccidiosis, and rapidly restoring the production performance of chicken flocks, thus improving breeding profits.

The invention discloses a compound traditional Chinese medicine and application thereof in preparing a medicine for resisting eimeria tenella or treating coccidiosis, and relates to the technical field of livestock breeding. The compound traditional Chinese medicine comprises the following raw materials in parts by mass: 5-20 parts of hesperidin and 1-10 parts of citric acid. According to the invention, chicks are taken as experimental subjects, a chick coccidiosis model is established, and the effect of the compound traditional Chinese medicine on the chick coccidiosis is researched from the aspects of general conditions of the chicks, bloody stool conditions, coccidiocyst discharge conditions, cecum tissue lesion conditions, pathological detection, serum biochemistry, oxidation resistance detection and the like. The compound traditional Chinese medicine achieves a good anti-coccidiosis drug level, the required raw materials are low in cost, convenient to use and free of hormones and antibiotics, no toxic or side effect is caused to chickens, drug resistance cannot be caused, a new choice is provided for replacing chemical drugs for preventing and treating coccidiosis, and addition of anti-coccidiosis drugs in chicken feed is expected to be reduced or replaced.

The present application relates to an anticoccidial composition comprising Garcinia mangostana and uses thereof. According to embodiments, the composition comprising Garcinia mangostana has an excellent effect of directly killing protozoa that can induce coccidiosis, has an effect of inhibiting penetration of cells by the protozoa, and / or has an effect of inhibiting proliferation of the protozoa in cells, and has an excellent effect on prevention, alleviation, and treatment of coccidiosis in vivo.

The present application relates to an anticoccidial composition comprising ginkgo leaves and use thereof. The composition comprising ginkgo leaves, according to an embodiment, has excellent effects of directly killing sporozoites that can induce coccidiosis, inhibiting the penetration of the sporozoites into cells, and / or inhibiting the proliferation of the sporozoites in cells, and has excellent effects of preventing, ameliorating and treating coccidiosis in vivo.

The invention discloses chicken-derived lactobacillus reuteri S3 for improving development of intestinal microorganisms of indigenous animals and application of the chicken-derived lactobacillus reuteri S3. The chicken-derived lactobacillus reuteri S3 can be used for preparing a chicken probiotic additive, and is used for improving the growth performance of chickens, enhancing the development of immune organs, promoting the colonization of beneficial bacteria of the chickens, enhancing the diversity of intestinal flora and relieving the harm of chicken coccidiosis infection. The strain is safe, harmless, green and environment-friendly when being continuously fed to chicks, has a remarkable growth promoting effect on the chicks, and can promote thymus development, so that the immunity of chicken bodies is enhanced. The strain can effectively relieve harm of eimeria tenella, eimeria giant and eimeria necatrix infection to chicks, and is characterized in that after the lactobacillus reuteri S3 is supplemented, clinical symptoms and intestinal tract lesions caused by the eimeria tenella infection can be relieved, the harm of the eimeria tenella infection to chick weight increment is relieved, coccidian oocyst discharge is reduced, and the chick weight increment rate is increased. The obvious prevention and control effects are realized on the chicken coccidiosis infection.

The present application relates to the technical field of drug resistance monitoring and management, and discloses a chicken coccidiosis drug resistance spatiotemporal drift deduction method and system, which comprises the following steps: step 1, receiving a multi-modal heterogeneous data set; step 2, constructing a phenotype feature vector according to the production performance recovery lag, recovery acceleration extreme value and fecal spectrum entropy; step 3, inputting the phenotype feature vector into a conditional reversible neural network to output a drug resistance genotype frequency distribution vector; step 4, taking the trained conditional reversible neural network as a teacher model, constructing a student model in the cloud, inputting the drug resistance genotype frequency distribution vector matrix and reputation weight into the student model, and outputting a global drug resistance characteristic map; step 5, constructing an asymmetric evolutionary game model to generate a drug resistance spatiotemporal evolution heat map; and step 6, inputting the regional average drug resistance gene frequency distribution into a reinforcement learning model to output a sequential drug use scheme.

The invention relates to the technical field of chicken flock immunization, and provides a method for promoting coccidiosis immune cycle by controlling the humidity of padding. The method comprises the following steps: laying a drip irrigation humidity control device; mixed padding is prepared, and the drip irrigation humidity control device is placed in the mixed padding; a water outlet hole of the drip irrigation humidity control device is arranged to be in direct contact with the ground, and the water outlet amount of the drip irrigation humidity control device is controlled, so that the humidity of the mixed padding is 20-30%. According to the method, the humidity of the mixed padding can be rapidly adjusted, and the humidity of each area of the padding is uniform. Besides, when the humidity of the padding is adjusted to be within the range, the sporulation rate of coccidiosis is superior to that of other padding humidity, secondary circulation of tender and toxic oocysts can be effectively guaranteed, and the tender and toxic immune protection effect is improved.

The present application relates to an anticoccidial composition comprising coumaric acid and use thereof. A composition comprising coumaric acid and / or a salt thereof, according to an embodiment, has excellent effects of inhibiting the cell penetration of protozoa that can induce coccidiosis and / or inhibiting the proliferation of the protozoa in cells, has excellent effects of preventing, ameliorating and treating coccidiosis in vivo, and remarkably reduces oocyst excretion in feces, thus being able to reduce secondary coccidiosis infection.

The present application relates to an anticoccidial composition comprising a stilbene-based compound, and a use thereof. A composition comprising a compound or a salt thereof, according to one embodiment, has excellent effects of direct killing of protozoa that can induce coccidiosis, inhibiting the cell penetration of the protozoa and / or inhibiting the intracellular proliferation of the protozoa, and preventing, alleviating, and treating in vivo coccidiosis.

The application discloses a plant source composition which is composed of eucalyptus leaf essential oil, eugenol essential oil and apigenin, and discloses a preparation method of the composition and application of the composition in resisting chicken coccidiosis, and belongs to the field of disease prevention and treatment of poultry and livestock and feed additive. The composition can resist Eimeria tenella, and has a promoting effect on the growth performance of chickens, is a new type of feed additive with small toxic and side effects and good insecticidal effect, has low production cost and good stability, and has great application prospect in the aspect of coccidiosis resistance of poultry.

The application discloses a traditional Chinese medicine compound with anti-coccidiosis effect and a preparation method and application thereof, and relates to the technical field of veterinary drugs. The traditional Chinese medicine compound comprises two or more components in radix sophorae flavescens, cusparia, spilanthes acmella, radix scutellariae and bai-cao-ceng, and the mass ratio is 1-10:1-5:1-10:1-5:1-5. In the traditional Chinese medicine compound, the components are combined together, have the effects of resisting insects, eliminating inflammation, stopping diarrhea and dysentery, stopping bleeding and resisting diseases, and have the advantages of low cost, safety, no drug resistance, environmental protection, no residue and no antibiotics, thereby providing a new idea for an antibiotic-free and green healthy breeding mode. In addition, the traditional Chinese medicine compound is simple to prepare and does not need other adjuvants, and can meet the demand of large-scale use in the breeding industry.

The invention provides a recombinant coccidiosis vector for expressing VP2 protein and a fluorescent label and a detection method of the recombinant coccidiosis vector, and belongs to the technical field of genetic engineering. The invention also provides sgRNA (small guide ribonucleic acid) targeting the ETH00009555 gene, and the sgRNA can be used for carrying out accurate gene fixed-point insertion; a gene editing system is established by sgRNA and homologous recombinant fragments, so that target fragments such as chicken infectious bursal disease virus VP2 protein and fluorescent label protein can be accurately subjected to homologous recombination on the ETH00009555 gene, and a recombinant coccidiosis vector for expressing the VP2 protein and a fluorescent label is constructed. The recombinant coccidium vector DNA is subjected to PCR (Polymerase Chain Reaction) detection and sequencing, and the result shows that the VP2 gene and the fluorescent tag gene are successfully recombined with the ETH00009555 gene; and the recombinant coccidiosis vector has an obvious fluorescence signal under a fluorescence microscope, which indicates that the recombinant coccidiosis vector successfully expresses the VP2 protein and the fluorescence tag protein.

The invention discloses a chicken coccidiosis detection method based on a YOLO-Cocci model, and has the following beneficial effects that a chicken coccidiosis data set suitable for a vaccine sample environment is constructed, a target detection model YOLO-Cocci with improved performance is provided based on a YOLOv8 model, the model can improve the accuracy of chicken coccidiosis detection in the vaccine sample environment, and the detection accuracy of chicken coccidiosis in the vaccine sample environment is improved. The accurate identification of different types of chicken coccidiosis and spore formation states thereof is realized; a contrast experiment result shows that the performance of the YOLO-Cocci model on a chicken coccidiosis data set is obviously superior to that of other target detection models, and an ablation experiment further verifies the superiority of the model provided by the invention in the aspect of detecting coccidiosis with similar forms; the YOLO-Cocci model provided by the invention not only provides key technical support for chicken coccidiosis detection in a vaccine sample environment, but also provides important reference basis for clinical detection and research of chicken coccidiosis.

The invention relates to an isospora hyis immunity-related molecular marker and application thereof, and belongs to the technical field of biology. The molecular marker comprises a CSF3 gene and / or an IFNG gene. According to the invention, through transcriptome analysis and qPCR, the reaction of the pig PBMC to the stimulant after artificial immunization is evaluated, the immune-related molecular marker and the stimulant are screened, and a foundation is laid for establishing a cell immunity determination method.

This invention relates to the field of feed technology, specifically to an anticoccidial plant extract, additives, and their applications. The anticoccidial plant extract of this invention is an aqueous extract of a combination of plant raw materials, including *Dichroa febrifuga*, *Portulaca oleracea*, *Cirsium japonicum*, *Platycladus orientalis* leaves, *Paeonia lactiflora*, *Astragalus membranaceus*, and licorice. This invention also proposes additives containing this plant extract, including feed additives and drinking water additives. This invention uses natural plant extracts to prevent and treat coccidiosis in poultry, exhibiting green and safe characteristics. Through the synergistic effect of the extracted components, it improves the growth performance of poultry infected with coccidia and reduces the number of coccidia oocysts in feces.

The invention relates to the technical field of drug resistance monitoring and treatment, and discloses a chicken coccidiosis drug resistance space-time drift deduction method and system, and the method comprises the following steps: 1, receiving a multi-mode heterogeneous data set; 2, according to the production performance recovery hysteresis, the recovery acceleration extreme value and the excrement spectrum entropy, constructing a phenotypic feature vector; 3, inputting the phenotype feature vector into a conditional reversible neural network, and outputting a drug-resistant genotype frequency distribution vector; 4, taking the trained conditional reversible neural network as a teacher model, constructing a student model at a cloud end, inputting the drug resistance genotype frequency distribution vector matrix and the reputation weight into the student model, and outputting a global drug resistance characteristic spectrum; 5, constructing an asymmetric evolutionary game model, and generating a drug resistance space-time evolution thermodynamic diagram; and 6, inputting the regional average drug resistance gene frequency distribution into the reinforcement learning model, and outputting a sequential drug use scheme.

The invention discloses a CPA primer group and a kit for detecting seven chicken eimeria coccidiosis and application of the CPA primer group and the kit, and belongs to the technical field of biological detection. Specific primers are designed for conservative COI genes of chicken eimeria, an optimal reaction system and 61 DEG C constant-temperature amplification conditions are optimized and determined, and a CPA detection method for detecting chicken eimeria is established. The method is extremely high in sensitivity, the lower limit of detection on a plasmid standard substance reaches 102 copies / L, extremely low pathogen load equivalent to 0.5 oocyst can be stably detected in practical application, the clinical sample verification coincidence rate reaches 100%, and a powerful technical support is provided for early warning, field monitoring and precise prevention and control of chicken coccidiosis.

The invention discloses an application of onion appendages and extracts thereof in preparation of veterinary drugs or feed additives for resisting parasitic diseases. A treatment test on chicken infected with coccidiosis proves that the coccidiosis resisting index of the onion skin extract can reach an efficient level close to 180 and is almost equivalent to that of a chemical medicine diclazuril, and pathological diseases show that the onion skin extract has remarkable effects of relieving cecum thickening and chicken bloody stool and improving intestinal tract pathological injury. The onion skin extract is prepared into a medicine feed additive, the medicine feed additive and feed are used for feeding sick chickens together, the chicken coccidiosis can be effectively prevented and treated, the effect is remarkable, and the market application and popularization value is achieved.

The application provides a lysophosphatidylcholine (LPC) kit with anti-coccidiosis effect, and discloses a diagnosis marker for Eimeria tenella infected chicken, which is characteristic phospholipid and sphingolipid molecules, and specifically phosphatidylcholine (PC), lysophosphatidylcholine (LPC) and sphingomyelin (SM). Meanwhile, the application provides a medicine composition for improving the survival rate of chicks infected with Eimeria tenella, which is characterized by containing effective amounts of 18:2 LPA, 18:1 LPC, 22:0 LPC, 16:0 18:1 PC, 16:0 18:2 PC and d18:1 16:0 SM. The application screens characteristic phospholipid and sphingolipid molecules in the cecum tissue of chicken infected with Eimeria tenella for the first time, and verifies the curative effect of phosphatidylcholine (PC), lysophosphatidylcholine (LPC) and sphingomyelin (SM) on acute infection of chicks with Eimeria tenella, which has great market development prospect.

This invention discloses a quadruple PCR primer set and kit for detecting chicken coccidiosis, belonging to the field of chicken coccidiosis biodetection technology. Compared with the prior art, this invention can simultaneously obtain detection results for four types of chicken coccidiosis, greatly shortening the detection and identification time and providing a guarantee for timely prevention of the spread of chicken coccidiosis. Furthermore, the coccidia oocyst lysis buffer provided by this invention can quickly obtain DNA templates for quadruple PCR detection. The quadruple PCR primers provided by this invention have high detection specificity, low detection limit, good repeatability, and reliable results.