98 results about "Coccidulini" patented technology

Echinacea dietary supplements are useful adjuvants for live anticoccidial vaccines. Feed supplementation with Echinacea preparations enhances the immune response to such vaccines. Echinacea purpurea in amounts of about 0.1% to 0.5% administered for about two weeks to day-old chicks has been found effective for providing significant weight gain compared to vaccination alone.

The invention relates a weakening agent of strong poisonous coccidian and process method, comprising following components: potassium bichromate, sodium chloride, sodium bicarbonate, glucose, potassium chloride, sodium nitride, and distilled water. Said weakening agent can weaken any kind of strong poisonous coccidian as E. tenena, taper, giant type, toxic type, and premature E. tenena. The chicklings injected this weakening agent is safe reliability and has high immunogenic. In addition, the invention use simple apparatus and can reduce cost 0.30 yuan every plumage, reduce mortality, increase laying rate and promote speed of growth compared to the method that preventing coccidian disease by drugs.

The invention discloses a preparation method of an eimeria tenella recombinant polypeptide vaccine VNQS. The preparation method comprises the following steps: preparing raw materials; the method comprises the following steps: 1, cloning a VKVQ encoding gene, designing an upstream primer shown in the formula SEQ ID NO: 3 and a downstream primer shown in the formula SEQ ID NO: 4 according to a chicken coccidiosis gene sequence, carrying out PCR amplification and electrophoresis to obtain a segment of about 1500bp, carrying out ligation transformation on the product to obtain VNQS of which the sequence overall length is 1452bp and of which the sequence is shown in the formula of SEQ ID NO: 1, and carrying out subsequent related steps. The vaccine prepared by the method can effectively controlchicken bodies to be infected with eimeria tenella by immunizing eimeria tenella recombinant polypeptide vaccine VNQS eukaryotic expression plasmids and prokaryotic expression proteins, so that the use amount of anti-coccidiosis drugs in chicken farms is greatly reduced, and chicken coccidiosis is effectively controlled.

The invention discloses an efficient green anti-coccidiosis feed for meat chickens and a preparation method and application thereof. The feed consists of corktree bark, baikal skullcap root, golden thread, dock root, licorice root, garden burnet root, betaine, vitamin A, vitamin K3, calcium hydrogen phosphate, potassium iodide and sodium selenite. The method comprises the following steps of: crushing the Chinese medicaments, sieving the powder with a sieve of 60 meshes, weighing the powder in a weight ratio, mixing for 5 to 10 minutes with an efficient mixer, packing with bags, wherein the mixing uniformity requirement of the mixer comprises that the variation coefficient of the uniformity is not more than 5 percent. The production process for the efficient green anti-coccidiosis feed is simple and easily operated and mastered. The feed is convenient to use and remarkable in effect; the coccidiosis incidence rate of the meat chickens can be reduced to less than 10 percent; and the feed can provide the requirements of the meat chickens on vitamins, trace elements and mineral in the growth and development processes at the same time of improving the coccidiosis resistance of the meat chickens, and has popularization and application value.

The invention discloses a coccidiosis oocyst cryoprotectant, and a preparation method and application thereof. The coccidiosis oocyst cryoprotectant comprises the following components: sodium dodecylsulfate, distearoyl phosphatidyl ethanolamine-polyethylene glycol, tartaric acid, ammonium sulfate, sucrose, fructose, sodium dihydrogen phosphate, disodium hydrogen phosphate, a standard PBS buffer solution and water for injection. The cryoprotectant is reasonable in formula, and can effectively inhibit ice crystal recrystallization in the cryopreservation process, regulate and control ice crystal morphology, reduce ice crystal growth rate and decrease damage of ice crystals to coccidiosis oocysts; and meanwhile, phospholipid molecules can be adsorbed to the walls of the coccidiosis oocysts,so the ice damage resistance of the coccidiosis oocysts is improved, and the coccidiosis oocysts can be stored in liquid nitrogen for a long time. Compared with a traditional cryoprotectant, the coccidiosis oocyst cryoprotectant of the invention does not contain toxic molecules such as DMSO and components such as expensive serum, avoids the toxic damage of DMSO to coccidiosis oocysts, lowers production cost, has effectively improved applicability in the field of coccidiosis vaccines is enhanced, and the cryoprotectant and is beneficial for wide popularization and application.

The invention relates to the technical field of animal coccidiosis vaccines, in particular to a rabbit coccidiosis live vaccine and a preparation method and application thereof, and provides a rabbitcoccidiosis live vaccine composition which comprises precocious attenuated strains of Eimeria magna, Eimeria intestinalis and Eimeria media and precocious attenuated strain of Eimeria perforans. The rabbit coccidiosis live vaccine has high immunoprotective efficacy and safety, can effectively prevent and treat rabbit coccidiosis and reduce or avoid using of coccidiostats, can remarkably reduce unfavorable impact of vaccine inoculation on growth and production performance like rabbit weight gain and has great economic benefit and wide application prospect. The invention further provides a breeding method of coccidium precocious attenuated strains. The breeding method can effectively shorten breeding period of precocious strains and improve breeding efficiency of the coccidium precocious attenuated strains, obtained precocious strains are stable in hereditary character and high in immunogenicity, and pathogenicity is remarkably lowered.

The invention provides a construction method for coccidia expressing exogenous fluorescent protein, and belongs to the technical field of gene engineering. The construction method for coccidia expressing exogenous fluorescent protein comprises the following steps: designing gRNA for a target gene of the coccidiosis, and constructing a gene editing plasmid containing the gRNA; constructing a homologous recombinant plasmid containing a target gene and an mCherry gene; co-transfecting the gene editing plasmid and the homologous recombinant plasmid into sporozoites of coccidia; and infecting animals with the transfected sporozoites, and performing screening to obtain the coccidia expressing exogenous fluorescent protein. A fluorescent protein mCherry gene and a screening gene are knocked intoa coccidia genome by combining a gene editing technology and a homologous recombination technology, and the constructed coccidia can stably and efficiently express a fluorescent signal. According to the construction method, not only can the coccidia expressing the exogenous fluorescent protein be successfully constructed, but also fluorescent protein expression is stable and high in efficiency, and the construction method is suitable for research on growth, genetic engineering or drug screening of coccidia.

The invention discloses an Eimeria tenella gene which is Et STK gene and includes an amino acid sequence shown as SEQ ID NO.1. Moreover, the invention provides a preparation method of a vaccine for prophylaxis and treatment of coccidiosis in chicken. The method includes: connecting the mature peptide of Eimeria tenella conserved protein Et STK with an expression vector of escherichia coli or pichia pastoris cell to obtain a recombinant expression vector, transferring the recombinant expression vector into a host of escherichia coli or pichia pastoris cell to obtain a recombination strain, and cultivating the recombination strain to obtain the vaccine of coccidiosis in chicken. The method has quite high application value.

The invention discloses a feed additive for preventing and treating coccidiosis in a culture process of spotted-brown breeder chickens. The feed additive is composed of, by weight, 55-65 parts of artemisia apiacea, 24-26 parts of antifebrile dichroa, 18-22 parts of amomum tsaoko, 28-32 parts of fresh ginger, 44-46 parts of radix bupleuri, 38-42 parts of radix paeoniae alba, 18-22 parts of liquorice, 255-265 parts of blending agents and 4-6 parts of ramulus mori additives. The feed additive has the functions of eliminating parasites, tonifying Qi, tonifying spleen, curing dysentery and helping digestion. An avian escherichia coli CVCC-245 bacterial strain bacteriostasis in vitro test indicates that the feed additive has a remarkable inhibiting effect to avian escherichia coli; the additive has a remarkable effect for preventing and treating chicken coccidiosis, the additive is tested on 120 thousand spotted-brown breeder chickens, and the average efficiency reaches over 97%. The feed additive has the great effect for preventing and treating chicken coccidial infection.

An adprin preparation for resisting coccidiosis comprises the following components, by mass, 4%-20% of adprin, 60%-85% of carrier and 6%-30% cosolvent. According to the adprin preparation for resisting the coccidiosis, the carrier is used for being combined with the cosolvent to enable the adprin to exist in a solid dispersion form, the adprin can be dispersed in water evenly and stably, the character is stable, and bioavailability is improved.

The invention relates to an injection containing gleptoferron and ponazuril. The injection further comprises pharmaceutically acceptable silicone oil, lecithin, a metal ion complexing agent, n-butyl alcohol, povidone, water and an optional pH regulator. The injection is stable, good in safety and high in bioavailability, and can be used for preventing and treating coccidiosis infection and iron-deficiency anemia of animals at the same time.

The invention provides a pharmaceutical composition for preventing and treating chicken coccidiosis and an application thereof. The pharmaceutical composition contains lactobacillus plantarum P8 and chlorogenic acid, wherein the biological preservation number of the lactobacillus plantarum P8 is CGMCC No. 6312. According to the invention, immune imbalance and intestinal oxidative damage of broilerchickens caused by coccidiosis infection can be relieved; the content of anti-inflammatory factors can be significantly improved, and the content of pro-inflammatory factors can be reduced, so the body immunity tends to be normal; meanwhile, the pharmaceutical composition provided by the invention can also relieve intestinal barrier damage, repair intestinal structures and enhance the resistanceof intestinal mucosa, is beneficial for intestinal health, can completely or partially replace chemically synthesized anticoccidial drugs and antibiotic products, has no drug residues and pollution, and is green, safe and environmentally-friendly.

The invention relates to the use of at least one acid stable protease in poultry feed in combination with anti-coccidial vaccination of birds for increasing the performance of vaccinated animals. It has been found surprisingly that the additions of at least one acid stable protease according to the invention to the diet of “cocci”-vaccinated birds have a significant improvement of animal performance. The inventors found that especially the use of serin proteases improve the performance of vaccinated broiler chickens, in particular the use of serin proteases increases weight gain and improves Feed Conversion Ratio (FCR) of cocci-vaccinated birds.

The invention relates to a suspension injection containing iron-dextran and ponazuril. The suspension injection is further prepared from pharmaceutically acceptable silicone oil, lecithin, a metal ion complexing agent, n-butyl alcohol, povidone, water and an optional pH regulator. The suspension injection is stable, good in safety and high in bioavailability, and capable of preventing and treating coccidiosis infection and iron-deficiency anemia of animals at the same time.

The invention discloses preparation and application of a fusion protein genetically engineered bacterium of coccidium antigen peptide/IL5. The amino acid sequence of the fusion protein is shown as SEQ ID No. 1. The sequence of the coding gene of the gene is shown as SEQ ID No.2. The protein provided by the invention has remarkable prevention, treatment and regulation effects on avian coccidiosis. The cationic polypeptide compound expressed by the food-grade bacillus subtilis causes acquired immune response of animal intestinal tracts through mucosal immunity, and an anti-coccidiosis antibody is generated. The interleukin 5 is an eosinophilic granulocyte activating factor and can activate eosinophilic granulocytes in intestinal tracts, secrete anti-parasitic active substances and repair intestinal mucosa damage caused by coccidium infection.

The invention discloses a cryoprotectant and a preparation method thereof, and application of the cryoprotectant in coccidiosis oocysts. The cryoprotectant comprises the following components: lauric acid, hydrogenated phosphatidylcholine, sodium fluoride, ammonium chloride, asparaginic acid, proline, cane sugar, sodium dihydrogen phosphate, disodium hydrogen phosphate, a PBS buffer solution and water for injection. The cryoprotectant is reasonable in formula, ice crystal recrystallization in the cryopreservation process can be effectively inhibited, ice crystal morphology is regulated and controlled, the ice crystal growth rate is reduced, and damage of ice crystals to coccidiosis oocysts is reduced; and meanwhile, phospholipid molecules can be adsorbed to the walls of the coccidiosis oocysts, so the ice damage resistance of the coccidiosis oocysts is improved, and the coccidiosis oocysts can be stored in liquid nitrogen for a long time. Compared with a traditional cryoprotectant, thecryoprotectant of the invention has the advantages that the cryoprotectant does not contain toxic molecules such as DMSO and components such as expensive serum, avoids the toxic damage of DMSO to coccidiosis oocysts, and is reduced in production cost, effectively improved in applicability in the field of coccidiosis vaccines and beneficial to wide popularization and application.