104 results about "Coccidulini" patented technology

The invention discloses a normal-temperature preparation method for ultrafine coccidia powder. The method comprises the following steps of: selecting decoction pieces of sweet wormwood, hairyvein agrimony, tuber fleeceflower root, Chinese pulsatilla root and cinnamon according to parts by weight, drying and smashing to obtain coarse powder of which the granularity is 80 meshes; putting the obtained coarse powder into a rod mill for smashing, feeding into the special bilateral airflow sieving machine through wind for sieving with a 500-mesh sieve, sieving to obtain power of which the particle diameter is less than or equal to 25 micrometers, conveying the powder which is obtained by sieving into a cyclone aggregator through airflow generated by a draught fan for collecting to obtain finished ultrafine coccidia powder; and collecting powder which is not sieved with the 500-mesh sieve with a funnel, and conveying to the rod mill through a pipeline for smashing circularly once again. The method has the advantages that: the entire preparation process is performed at the normal temperature without low temperature or special additional conditions; the particle diameter of a prepared finished medicament is less than or equal to 25 micrometers, and the cell-wall breaking rate and the biological availability are greatly increased; and due to the adoption of the bilateral airflow sievingmachine, the preparation process of the ultrafine coccidia powder is simplified, and the aim of controlling the quality standard of Chinese medicinal powder is fulfilled.

The invention relates to the field of veterinary drugs, and particularly relates to prevention and cure of three main parasitic diseases, namely a tapeworm disease, a roundworm disease and a coccidium disease, or the tapeworm and coccidium mixed infection and the roundworm and coccidium mixed infection of free-ranging chickens. The invention particularly discloses a traditional Chinese medicine composite for resisting the parasites of the free-ranging chickens and a preparation method thereof. The traditional Chinese medicine composite is prepared into a traditional Chinese medicine formulation by taking rangooncreeper fruits, aloes, liquorice, hawthorns, fructus carpesii, medicated leaven, fructus hordei germinatus, azedarach, rhizoma atractylodis, codonopsis pilosula, betel nuts, radix dichroae, cyrtomium fortunei, radix astragali and pericarpium citri reticulatae as crude drugs. The traditional Chinese medicine disclosed by the invention is used for treating the three main parasitic diseases, namely the tapeworm disease, the roundworm disease and the coccidium disease, or the tapeworm and coccidium mixed infection and the roundworm and coccidium mixed infection of the free-ranging chickens, enhances the feed conversion rate, strengthens the organism resistance action and has the advantages of outstanding effect, safety in use, no residue, difficulty in drug resistance generation, low price, and the like.

The present invention relates to a coccidiosis vaccine to protect poultry against Eimeria infection, comprising a recombinant avian adenovirus vector comprising in frame a (heterologous) promoter linked to a hydrophobic signal sequence for membrane anchoring, or linked to an hydrophobic secretion signal and a cleavage site to allow secretion; a multiple cloning site for in frame insertion of an Eimeria antigen ORF such as derived from the r56, 82 kDa, and / or TFP250 antigens; a polyadenylation signal; and an avian adenovirus genome.

The invention relates to a decoquinate nanoemulsion coccidium-resisting drug. The particle size of the decoquinate nanoemulsion coccidium-resisting drug is 1-100nm, the decoquinate nanoemulsion coccidium-resisting drug consists of, by mass percentage, decoquinate 0.1-6.0%, surface active agent 20.0-35.0%, cosurfactant 0-22.0%, cosolvent 1.0-3.0%, oil 1.5-12.0% and the balance distilled water. The drug is light yellow, is clear and transparent liquid visually, has the advantages of being good in stability, low in viscosity, strong in dispersibility, rapid in absorption and the like, and further has slow releasing and targeting effects. The bioavailability of the drug can be improved, half-life period of the drug in the body can be prolonged, and curative effect is enhanced. The decoquinate nanoemulsion coccidium-resisting drug is simple in preparation process, low in energy consumption, small in toxicity and high in safety, and can be produced in a mass mode without requiring special equipment.

The invention discloses an industrialized production method for separating and purifying a chicken eimeria coccidium oocyst. According to the industrialized production method, the separation and purification of the chicken eimeria coccidium oocyst are carried out by adopting the scientific assembly of a disc separator and a tubular type centrifuge, after sporulated oocysts are purified, larger impurities and extremely tiny impurities are not generated; an object plate is thoroughly subjected to microscopic examination, unsporulated oocysts are also all thoroughly removed, sporulated oocysts are all in the visual field, and the solution background is clear. The industrial production method disclosed by the invention can be used for shortening the separation and purification time of the oocysts; compared with the traditional technology, the industrial production method disclosed by the invention can be used for saving the time by more than 70%, reducing the product instability caused by excessive human factors by preventing integral oocyst purification from the disturbance of excessive artificial working and is the breakthrough of the existing coccidium vaccine production method; the industrial production method can be used for continuous production by only needing two centrifuges, thereby greatly reducing the cost input of an enterprise.

A method for protecting fowl against coccidiosis includes the steps of inoculating the fowl with coccidial oocysts from a strain of Eimeria that is at least partially resistant to an ionophore and subsequently administering to the fowl the ionophore and at least one chemotherapeutic anticoccidial agent. In particular, the ionophore is administered in stages both before and after the administration of the at least on chemotherapeutic agent. In a preferred embodiments, the method provides protection from coccidiosis without administration of antibiotics to the fowl and without continued administration of agents throughout the life of the fowl.

A method for obtaining an immunogenic strain useful for producing a vaccine against Coccidiosis comprises the cycle of infecting at least one first group of specific pathogen-free donor birds with oocysts from an Eimeria species. Blood is then collected from these donor birds, and is then used to infect a second group of specific pathogen-free birds. Oocysts are collected from the second group of birds. These oocysts are then multiplicated to complete the cycle. The cycle is then repeated using the multiplicated oocysts. After a total of about three cycles, a final antigen may be harvested and utilized as a source to generate oocysts for a vaccine.

Echinacea dietary supplements are useful adjuvants for live anticoccidial vaccines. Feed supplementation with Echinacea preparations enhances the immune response to such vaccines. Echinacea purpurea in amounts of about 0.1% to 0.5% administered for about two weeks to day-old chicks has been found effective for providing significant weight gain compared to vaccination alone.

The invention discloses a multivalence recombinant protein vaccine for chicken coccidiosis, and a preparation method and application of the multivalence recombinant protein vaccine for chicken coccidian. The multivalence recombinant protein vaccine for chicken coccidiosis comprises 4 chicken coccidian proteins: an eimeria tenella rhoptry protein 41, an eimeria tenella gametophyte protein 22, an eimeria maxima gametophyte protein 56 and an eimeria acervulina merozoite protein Ea3-1E. The four chicken coccidian proteins are respectively connected with escherichia coli expression carriers to obtain recombinant expression carriers for expressing recombinant proteins. Different recombinant proteins are mixed with an adjuvant in proportion, when descendant chicks of immunized hens are inoculatedwith coccidian are compared with descendant chicks of hens which are not immunized, the survival rate and the relative weight increment of chicks inoculated with the coccidian can be effectively increased, the discharge quantity of oocysts is reduced, and favorable maternal immunity effects are shown.

The invention relates to a maduramicin ammonium nicarbazin premix and a preparation method thereof. The premix is prepared by dissolving maduramicin ammonium in a corresponding solvent, and then spraying the solution onto nicarbazin and an auxiliary material that are uniformly mixed in a fluidized bed. The solvent can be one or more of a 40%-95% ethanol solution, benzyl alcohol, ethyl acetate and the like. The auxiliary material can be selected from the following: corn starch, plaster stone, corncob powder, anhydrous glucose, talcum powder, and calcium carbonate, etc. The combined use of maduramicin ammonium and nicarbazin can achieve a synergistic anticoccidial effect, not only can inhibit coccidia growth, but also can kill coccidian. The premix is mainly used for prevention and control of chicken coccidiosis. The preparation method of the premix is simple and feasible, has a very good uniform mixing effect, and can effectively avoid various degrees of chicken poisoning phenomena caused by uneven mixing.

[Objects] An object of the present invention is to provide an orally administrable agent for medical use for animals for preventing or treating coccidiosis which is highly safe with no side effects, an agent for a food or a drink for use in the preservation of health, a feed additive, and a feed comprising the same. Another object of the present invention is to provide a method for rearing animals (particularly, livestock and poultry) using the same and a method for controlling coccidiosis using the same.[Means for Resolution] By using a fermentation product of a lactic acid bacterium and / or a whey fermentation product of a propionic acid bacterium as an active ingredient, an agent for preventing or treating coccidiosis, an agent for use in the preservation of health, and a feed additive can be provided.

The invention relates a weakening agent of strong poisonous coccidian and process method, comprising following components: potassium bichromate, sodium chloride, sodium bicarbonate, glucose, potassium chloride, sodium nitride, and distilled water. Said weakening agent can weaken any kind of strong poisonous coccidian as E. tenena, taper, giant type, toxic type, and premature E. tenena. The chicklings injected this weakening agent is safe reliability and has high immunogenic. In addition, the invention use simple apparatus and can reduce cost 0.30 yuan every plumage, reduce mortality, increase laying rate and promote speed of growth compared to the method that preventing coccidian disease by drugs.

The invention discloses a preparation method of an eimeria tenella recombinant polypeptide vaccine VNQS. The preparation method comprises the following steps: preparing raw materials; the method comprises the following steps: 1, cloning a VKVQ encoding gene, designing an upstream primer shown in the formula SEQ ID NO: 3 and a downstream primer shown in the formula SEQ ID NO: 4 according to a chicken coccidiosis gene sequence, carrying out PCR amplification and electrophoresis to obtain a segment of about 1500bp, carrying out ligation transformation on the product to obtain VNQS of which the sequence overall length is 1452bp and of which the sequence is shown in the formula of SEQ ID NO: 1, and carrying out subsequent related steps. The vaccine prepared by the method can effectively controlchicken bodies to be infected with eimeria tenella by immunizing eimeria tenella recombinant polypeptide vaccine VNQS eukaryotic expression plasmids and prokaryotic expression proteins, so that the use amount of anti-coccidiosis drugs in chicken farms is greatly reduced, and chicken coccidiosis is effectively controlled.

The invention discloses an efficient green anti-coccidiosis feed for meat chickens and a preparation method and application thereof. The feed consists of corktree bark, baikal skullcap root, golden thread, dock root, licorice root, garden burnet root, betaine, vitamin A, vitamin K3, calcium hydrogen phosphate, potassium iodide and sodium selenite. The method comprises the following steps of: crushing the Chinese medicaments, sieving the powder with a sieve of 60 meshes, weighing the powder in a weight ratio, mixing for 5 to 10 minutes with an efficient mixer, packing with bags, wherein the mixing uniformity requirement of the mixer comprises that the variation coefficient of the uniformity is not more than 5 percent. The production process for the efficient green anti-coccidiosis feed is simple and easily operated and mastered. The feed is convenient to use and remarkable in effect; the coccidiosis incidence rate of the meat chickens can be reduced to less than 10 percent; and the feed can provide the requirements of the meat chickens on vitamins, trace elements and mineral in the growth and development processes at the same time of improving the coccidiosis resistance of the meat chickens, and has popularization and application value.

The invention discloses a coccidiosis oocyst cryoprotectant, and a preparation method and application thereof. The coccidiosis oocyst cryoprotectant comprises the following components: sodium dodecylsulfate, distearoyl phosphatidyl ethanolamine-polyethylene glycol, tartaric acid, ammonium sulfate, sucrose, fructose, sodium dihydrogen phosphate, disodium hydrogen phosphate, a standard PBS buffer solution and water for injection. The cryoprotectant is reasonable in formula, and can effectively inhibit ice crystal recrystallization in the cryopreservation process, regulate and control ice crystal morphology, reduce ice crystal growth rate and decrease damage of ice crystals to coccidiosis oocysts; and meanwhile, phospholipid molecules can be adsorbed to the walls of the coccidiosis oocysts,so the ice damage resistance of the coccidiosis oocysts is improved, and the coccidiosis oocysts can be stored in liquid nitrogen for a long time. Compared with a traditional cryoprotectant, the coccidiosis oocyst cryoprotectant of the invention does not contain toxic molecules such as DMSO and components such as expensive serum, avoids the toxic damage of DMSO to coccidiosis oocysts, lowers production cost, has effectively improved applicability in the field of coccidiosis vaccines is enhanced, and the cryoprotectant and is beneficial for wide popularization and application.

The invention relates to the technical field of animal coccidiosis vaccines, in particular to a rabbit coccidiosis live vaccine and a preparation method and application thereof, and provides a rabbitcoccidiosis live vaccine composition which comprises precocious attenuated strains of Eimeria magna, Eimeria intestinalis and Eimeria media and precocious attenuated strain of Eimeria perforans. The rabbit coccidiosis live vaccine has high immunoprotective efficacy and safety, can effectively prevent and treat rabbit coccidiosis and reduce or avoid using of coccidiostats, can remarkably reduce unfavorable impact of vaccine inoculation on growth and production performance like rabbit weight gain and has great economic benefit and wide application prospect. The invention further provides a breeding method of coccidium precocious attenuated strains. The breeding method can effectively shorten breeding period of precocious strains and improve breeding efficiency of the coccidium precocious attenuated strains, obtained precocious strains are stable in hereditary character and high in immunogenicity, and pathogenicity is remarkably lowered.

The invention provides an eimeria tenella AN1-like Zinc finger domain-containing protein and an application thereof in inhibiting invasion of coccidium. The eimeria tenella AN1-like Zinc finger domain-containing protein is cloned, expressed and purified. The transcription level of EtAN1-ZnFP in different developmental stages of polypides is detected by fluorescence quantitative PCR (qPCR). The immune protective effect of EtAN1-ZnFP after chickens are infected with coccidium is evaluated by immunoprotection experiments. Research results indicate that the EtAN1-ZnFP is possible to participate ininvasion and growing development of eimeria tenella.

An anticoccidial disinfection formulation containing a compound represented by general formula (I) below as an active ingredient:R1—N═C═S   (I)[In formula (I), R1 represents an alkenyl group having 2 to 8 carbon atoms or the like] This compound has a high sporulation inhibitory activity against coccidian oocysts and is thus effective for eradicating coccidians and for disinfecting rearing facilities and rooms of host animals, such as a livestock barn and a poultry house.

The invention provides a construction method for coccidia expressing exogenous fluorescent protein, and belongs to the technical field of gene engineering. The construction method for coccidia expressing exogenous fluorescent protein comprises the following steps: designing gRNA for a target gene of the coccidiosis, and constructing a gene editing plasmid containing the gRNA; constructing a homologous recombinant plasmid containing a target gene and an mCherry gene; co-transfecting the gene editing plasmid and the homologous recombinant plasmid into sporozoites of coccidia; and infecting animals with the transfected sporozoites, and performing screening to obtain the coccidia expressing exogenous fluorescent protein. A fluorescent protein mCherry gene and a screening gene are knocked intoa coccidia genome by combining a gene editing technology and a homologous recombination technology, and the constructed coccidia can stably and efficiently express a fluorescent signal. According to the construction method, not only can the coccidia expressing the exogenous fluorescent protein be successfully constructed, but also fluorescent protein expression is stable and high in efficiency, and the construction method is suitable for research on growth, genetic engineering or drug screening of coccidia.

The invention relates to polypeptides for treating chicken coccidiosis, in particular to a polypeptide for preventing and treating eimeria tenella and a pharmaceutical composition containing the polypeptide. The polypeptide has no obvious toxic effect on a host and has no pollution to the environment. Tests prove that the MIC50 of the polypeptide to eimeria tenella is 63.15 [mu]g / ml, and the polypeptide has a good eimeria tenella resisting effect.

The invention discloses an Eimeria tenella gene which is Et STK gene and includes an amino acid sequence shown as SEQ ID NO.1. Moreover, the invention provides a preparation method of a vaccine for prophylaxis and treatment of coccidiosis in chicken. The method includes: connecting the mature peptide of Eimeria tenella conserved protein Et STK with an expression vector of escherichia coli or pichia pastoris cell to obtain a recombinant expression vector, transferring the recombinant expression vector into a host of escherichia coli or pichia pastoris cell to obtain a recombination strain, and cultivating the recombination strain to obtain the vaccine of coccidiosis in chicken. The method has quite high application value.

The invention discloses a feed additive for preventing and treating coccidiosis in a culture process of spotted-brown breeder chickens. The feed additive is composed of, by weight, 55-65 parts of artemisia apiacea, 24-26 parts of antifebrile dichroa, 18-22 parts of amomum tsaoko, 28-32 parts of fresh ginger, 44-46 parts of radix bupleuri, 38-42 parts of radix paeoniae alba, 18-22 parts of liquorice, 255-265 parts of blending agents and 4-6 parts of ramulus mori additives. The feed additive has the functions of eliminating parasites, tonifying Qi, tonifying spleen, curing dysentery and helping digestion. An avian escherichia coli CVCC-245 bacterial strain bacteriostasis in vitro test indicates that the feed additive has a remarkable inhibiting effect to avian escherichia coli; the additive has a remarkable effect for preventing and treating chicken coccidiosis, the additive is tested on 120 thousand spotted-brown breeder chickens, and the average efficiency reaches over 97%. The feed additive has the great effect for preventing and treating chicken coccidial infection.

An adprin preparation for resisting coccidiosis comprises the following components, by mass, 4%-20% of adprin, 60%-85% of carrier and 6%-30% cosolvent. According to the adprin preparation for resisting the coccidiosis, the carrier is used for being combined with the cosolvent to enable the adprin to exist in a solid dispersion form, the adprin can be dispersed in water evenly and stably, the character is stable, and bioavailability is improved.

The invention relates to an injection containing gleptoferron and ponazuril. The injection further comprises pharmaceutically acceptable silicone oil, lecithin, a metal ion complexing agent, n-butyl alcohol, povidone, water and an optional pH regulator. The injection is stable, good in safety and high in bioavailability, and can be used for preventing and treating coccidiosis infection and iron-deficiency anemia of animals at the same time.

The invention discloses anti-coccidiosis multivalent recombinant protein IgY (Immunoglobulin of yolk) as well as a preparation method and an application thereof. The preparation method of the anti-coccidiosis multivalent recombinant protein IgY comprises the steps as follows: linking EtROP41, EtGAM22, EmGAM56 and Ea3-1E with Escherichia coli expression vectors respectively to obtain recombinant expression vectors, expressing recombinant proteins, mixing different recombinant proteins with adjuvants in a certain proportion, immunizing hens, collecting eggs to prepare IgY. Compared with the control group, after the prepared IgY is used for preventing and treating coccidiosis of chicks, the IgY can increase the survival rate and relative weight gain rate of the chicks and reduce excretion ofoocysts, and shows a good anti-coccidiosis effect and the application prospect in treatment and prevention of coccidiosis.

The invention relates to a method of obtaining and purifying 2-(2-nitrovinyl)furane consisting in reacting furfural and nitromethane, using isobutylamine as a catalyst and activated carbon as an adsorbent, in order to obtain a product of pharmaceutical quality. Said technique considerably reduces the environmental impact by eliminating the gaseous waste from the system by means of absorption in water. The inventive pharmaceutical composition contains 2-(2-nitrovinyl)furan and has powerful action against low concentrations of coccidia.

The invention relates to the use of at least one acid stable protease in poultry feed in combination with anti-coccidial vaccination of birds for increasing the performance of vaccinated animals. It has been found surprisingly that the additions of at least one acid stable protease according to the invention to the diet of “cocci”-vaccinated birds have a significant improvement of animal performance. The inventors found that especially the use of serin proteases improve the performance of vaccinated broiler chickens, in particular the use of serin proteases increases weight gain and improves Feed Conversion Ratio (FCR) of cocci-vaccinated birds.

The invention relates to a suspension injection containing iron-dextran and ponazuril. The suspension injection is further prepared from pharmaceutically acceptable silicone oil, lecithin, a metal ion complexing agent, n-butyl alcohol, povidone, water and an optional pH regulator. The suspension injection is stable, good in safety and high in bioavailability, and capable of preventing and treating coccidiosis infection and iron-deficiency anemia of animals at the same time.