A feed additive and compound feed for preventing and treating broiler chicken coccidian infection

Abstract

This invention provides a feed additive and compound feed for preventing and treating coccidiosis in broilers, comprising the following components: *Kluyveromyces martensii*, ferulic acid esterase, aminopeptidase, and glucanase. Preferably, the mass percentage of *Kluyveromyces martensii* is 30-40%; the mass percentage of ferulic acid esterase is 15-28%; the mass percentage of aminopeptidase is 15-25%; and the mass percentage of glucanase is 18-25%. Based on extensive research, this invention has determined that this combination has a significant effect on coccidiosis control in broilers, effectively improving broiler production performance indicators, increasing economic benefits, and reducing the use of anticoccidial drugs.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of feed additives, in particular to a feed additive for preventing and treating chicken coccidiosis and a compound feed. BACKGROUND

[0002] Chicken coccidiosis is a major obstacle to the large-scale development of the broiler industry. The occurrence of infectious coccidiosis can significantly reduce the intestinal health of broilers, reduce the production performance of broilers, and even cause the death of broilers. The prevention and treatment of broiler coccidiosis is currently mainly through the use of antibiotics and chemically synthesized drugs, as well as the injection of immune vaccines. Long-term and large-scale use of antibiotics and chemically synthesized drugs will lead to drug resistance of coccidia strains, and at the same time, it will lead to drug residues in broiler meat products, threatening the health of consumers.

[0003] Chicken coccidiosis has the strongest pathogenicity of Eimeria tenella, which parasitizes in the cecum and is commonly known as cecal coccidiosis. It often occurs in 21-50-day-old chicks, and the mortality rate can sometimes reach more than 80%. The prevention and treatment of coccidia by microecological preparations and enzyme preparations is a new direction for the prevention and treatment of coccidia at the present stage of broiler breeding, and has obvious effects on the prevention and control of coccidia in broilers, which can improve the production indexes of broilers, economic benefits, and reduce the use of anticoccidial drugs.

[0004] In the prior art, the existing common feed additives for preventing and treating chicken coccidiosis, such as traditional Saccharomyces cerevisiae and Chinese herbal medicine, have the following disadvantages: Saccharomyces cerevisiae does not contain two genes LAC12 and LAC4 for lactose metabolism, and thus cannot utilize lactose and express ferulic acid esterase; the effective components of Chinese herbal medicine are not clear, and the safe treatment amount range of Chinese medicine is not clear.

[0005] Based on the above problems, it is necessary to develop a new feed for preventing and treating chicken coccidiosis. Therefore, the present application provides a new feed additive for preventing and treating chicken coccidiosis and a compound feed. SUMMARY

[0006] Based on the above description, the present application provides a feed additive for preventing and treating chicken coccidiosis and a compound feed to overcome the problems of poor effect of existing antibiotics in preventing and treating chicken coccidiosis and safety hazards caused by long-term use.

[0007] The technical solution of the present application to solve the above technical problems is as follows:

[0008] In a first aspect, the present application provides a feed additive for preventing and treating chicken coccidiosis, which comprises the following components: Maxx Kluyveromyces, ferulic acid esterase, aminopeptidase and dextranase.

[0009] Based on the above technical solution, the present application can be further improved as follows.

[0010] Further, the mass percentage of the Kluyveromyces marxianus is 30-40%;

[0011] The mass percentage of the ferulic acid esterase is 15-28%;

[0012] The mass percentage of the aminopeptidase is 15-25%;

[0013] The mass percentage of the glucanase is 18-25%.

[0014] Further, the mass percentage of the Kluyveromyces marxianus is 35%;

[0015] The mass percentage of the ferulic acid esterase is 20%;

[0016] The mass percentage of the aminopeptidase is 20%;

[0017] The mass percentage of the glucanase is 25%.

[0018] Further, the bacterial activity of the Kluyveromyces marxianus is 150-500 billion / g;

[0019] The enzyme activity of the ferulic acid esterase is 50-2000 U;

[0020] The enzyme activity of the aminopeptidase is 500-30000 U;

[0021] The enzyme activity of the glucanase is 1000-300000 U.

[0022] Further, the bacterial activity of the Kluyveromyces marxianus is 200 billion / g;

[0023] The enzyme activity of the ferulic acid esterase is 100 U;

[0024] The enzyme activity of the aminopeptidase is 800 U;

[0025] The enzyme activity of the glucanase is 2000 U.

[0026] In the second aspect, the application provides a compound feed, comprising: the additive amount of the feed additive is 420-550 g / t of the mass of the poultry daily ration.

[0027] On the basis of the above technical solution, the application can also be improved as follows.

[0028] Further, the additive amount of the feed additive is 500 g / t of the mass of the poultry daily ration.

[0029] Compared with the prior art, the technical solution of the application has the following beneficial technical effects:

[0030] The feed additive for preventing and treating broiler chicken coccidiosis provided by the present application is composed of four components: Kluyveromyces marxianus, feruloyl esterase, aminopeptidase and glucanase. Among them, Kluyveromyces marxianus (KM) is a food safety yeast, which can be used as probiotics to improve the disease resistance and growth performance of animals; feruloyl esterase refers to an enzyme that can hydrolyze the ester bond in methyl ferulate, oligosaccharide ferulate and polysaccharide ferulate to free ferulic acid, which can cut the cross-linking between polysaccharide-polysaccharide and polysaccharide-lignin in the cell wall, which is beneficial to the degradation of polysaccharide and the release of lignin in the cell wall material; ferulic acid has the effects of inhibiting bacteria and reducing inflammation as a functional substance; aminopeptidase is a kind of exoprotease that can selectively remove amino acid residues from the N-terminal of protein and polypeptide to produce free amino acid, which can hydrolyze protein in depth, remove the bitterness of protein hydrolysate and produce bioactive polypeptide; glucanase can effectively decompose beta-glucan in plant cell wall, reduce the content of non-starch polysaccharide and its anti-nutritional factors, and improve the growth rate and feed conversion efficiency of livestock and poultry.

[0031] There is no related research on the application of Kluyveromyces marxianus, feruloyl esterase, aminopeptidase and glucanase in the prevention and treatment of broiler chicken coccidiosis in the prior art. Based on a large amount of work, the present application explores this combination. Compared with the prior art, the present application is easy to apply, the effect is stable, the drug efficacy of the feed can be maintained for a long time, the pressure of the chickens contacting the drugs is reduced, the components are clear and clear, there is no drug resistance and no residue, the coccidiosis prevention and control has obvious effect on broiler chickens, effectively improves the production performance index of broiler chickens, improves the economic benefit, and reduces the use of anticoccidial drugs. DETAILED DESCRIPTION

[0032] The embodiments of the present application will be further described in detail. Obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict.

[0033] The content disclosed in the present application can be improved in material, method and reaction condition at the same time, and all these improvements shall fall within the spirit and scope of the present application. If not specifically indicated, the technical means used in the examples is the conventional means known by those skilled in the art. The reagents and biological materials can be obtained from commercial channels if not specially indicated; the databases used are all public online databases.

[0034] The feed additive for preventing and treating broiler chicken coccidiosis provided by the present application includes the following components: Kluyveromyces marxianus, feruloyl esterase, aminopeptidase and glucanase.

[0035] Optionally, the mass percentage of each component is respectively:

[0036] The mass percentage of the Kluyveromyces marxianus is 30-40%;

[0037] The mass percentage of the ferulic acid esterase is 15-28%;

[0038] The mass percentage of the aminopeptidase is 15-25%;

[0039] The mass percentage of the glucanase is 18-25%.

[0040] Optionally, the enzyme activity of each component is respectively:

[0041] The bacterial activity of the Kluyveromyces marxianus is 150-500 billion / g;

[0042] The enzyme activity of the ferulic acid esterase is 50-2000 U;

[0043] The enzyme activity of the aminopeptidase is 500-30000 U;

[0044] The enzyme activity of the glucanase is 1000-300000 U.

[0045] The technical effects of the feed additive for preventing and treating chicken coccidian infection provided by the present application are further illustrated in combination with the following examples and comparative examples.

[0046] Example 1

[0047] The present example provides a feed additive for preventing and treating chicken coccidian infection, which comprises the following components in terms of mass percentage: Kluyveromyces marxianus 35%, ferulic acid esterase 20%, aminopeptidase 20%, and glucanase 25%.

[0048] In the present example, the enzyme activity of each single enzyme in each gram of the feed additive is as follows: Kluyveromyces marxianus 200 billion, ferulic acid esterase 100 U, aminopeptidase 800 U, and glucanase 2000 U.

[0049] Example 2

[0050] The present example provides a feed additive for preventing and treating chicken coccidian infection, which comprises the following components in terms of mass percentage: Kluyveromyces marxianus 31%, ferulic acid esterase 15%, aminopeptidase 17%, and glucanase 19%.

[0051] In the present example, the enzyme activity of each single enzyme in each gram of the feed additive is as follows: Kluyveromyces marxianus 200 billion, ferulic acid esterase 100 U, aminopeptidase 800 U, and glucanase 2000 U.

[0052] Example 3

[0053] The embodiment provides a feed additive for preventing and treating broiler coccidiosis, which comprises the following components in percentage by mass: 35% of Maxx Kluyveromyces, 20% of ferulic acid esterase, 20% of aminopeptidase, and 25% of glucanase.

[0054] In the feed additive, the enzyme activity of each single enzyme is as follows: 80 billion of Maxx Kluyveromyces, 350 U of ferulic acid esterase, 1080 U of aminopeptidase, and 5000 U of glucanase.

[0055] Comparative Example 1

[0056] Compared with the embodiment 1, the comparative example provides a feed additive, which only comprises Maxx Kluyveromyces with a bacterial activity of 200 billion / g.

[0057] Comparative Example 2

[0058] Compared with the embodiment 1, the comparative example provides a feed additive, which only comprises ferulic acid esterase with an enzyme activity of 100 U / g.

[0059] Comparative Example 3

[0060] Compared with the embodiment 1, the comparative example provides a feed additive, which only comprises aminopeptidase with an enzyme activity of 800 U / g.

[0061] Comparative Example 4

[0062] Compared with the embodiment 1, the comparative example provides a feed additive, which only comprises glucanase with an enzyme activity of 2000 U / g.

[0063] Comparative Example 5

[0064] Compared with the embodiment 1, the comparative example provides a feed additive, which does not comprise Maxx Kluyveromyces, and comprises 35% of ferulic acid esterase, 25% of aminopeptidase, and 40% of glucanase.

[0065] In the feed additive, the enzyme activity of each single enzyme is as follows: 100 U of ferulic acid esterase, 800 U of aminopeptidase, and 2000 U of glucanase.

[0066] Comparative Example 6

[0067] Compared with the embodiment 1, the comparative example provides a feed additive, which comprises 35% of Saccharomyces cerevisiae, 20% of ferulic acid esterase, 20% of aminopeptidase, and 25% of glucanase.

[0068] Comparative Example 7

[0069] The comparative example provides a commercially available product as a comparison, and the commercially available product is a coccidiosis vaccine: a generic name of a tetravalent live vaccine for chicken coccidiosis, which is produced by Foshan Zhengdian Biotechnology Co., Ltd.

[0070] Effect verification:

[0071] The prevention and treatment effects of the feed additive of the present application on broilers infected with coccidia were verified. The feed additive obtained in the above examples and comparative examples was mixed with poultry daily ration to obtain corresponding broiler feed. The poultry daily ration herein can be selected as corn-soybean meal basic daily ration.

[0072] Further verification tests were carried out, and the specific test process was as follows:

[0073] In this test, 720 1-day-old AA white-feathered broilers with similar body weight and health were selected and randomly divided into 12 groups, with 5 replicates per group, 12 broilers per replicate (male and female were distinguished by speed difference, and the male to female ratio was 1:1), and the broilers were raised on the net.

[0074] The feed of the two control groups was corn-soybean meal basic daily ration (one of which was the coccidia group).

[0075] According to the above examples 1 to 3 and comparative examples 1 to 7, the corresponding feed additive was added to the corn-soybean meal basic daily ration, and the addition amount was 500 g / t.

[0076] The test lasted for 21 days, and during the whole test period, the chickens were fed with different daily rations according to different treatments.

[0077] At 15 days of age, the broilers in one of the control groups and all the treatment groups were infected with coccidia by gavage, 1x10 5 (1 mL / bird) of Eimeria tenella oocysts per chicken; the control group not infected by gavage was given the same volume of normal saline per chicken. Comparative example 7 was immunized with the vaccine one week before artificial infection of chicken coccidia.

[0078] The production performance and average oocyst number per gram of feces of broilers were determined.

[0079] Table 1 21d broiler growth performance

[0080]

[0081] Table 2 Average oocyst number per gram of feces of broilers

[0082] Item Average oocysts per gram of feces Control group 20.56 Coccidia group 2.03 x 10 6 ]] Example 1 232 Example 2 394 Example 3 273 Comparative Example 1 1.21 x 10 3 ]] Comparative Example 2 1.08 x 10 3 ]] Comparative Example 3 4.30 x 10 4 ]] Comparative Example 4 3.54 x 10 4 ]] Comparative Example 5 776 Comparative Example 6 694 Comparative Example 7 220

[0083] In combination with the above table 1 and table 2, it can be seen that:

[0084] (1) Compared with the control group, the average daily feed intake and average daily gain of the coccidia group were significantly reduced by 22.85% (P<0.05) and 38.32% (P<0.05), respectively, and the feed conversion ratio was significantly increased by 25.85% (P<0.05).

[0085] (2) The growth performance of the examples is obviously superior to the comparative examples, and the growth performance of the comparative examples is superior to the coccidia group, especially the average daily feed intake and the average daily weight gain of the examples are significantly improved, and the feed conversion ratio of the examples is significantly reduced, wherein the broiler growth performance of the example 1 is the best, compared with the coccidia group, the average daily feed intake and the average daily weight gain are significantly increased by 28.65% (P<0.05) and 63.67% (P<0.05) respectively, and the feed conversion ratio is reduced by 22.16% (P<0.05).

[0086] (3) Compared with the control group, the average number of oocysts per gram of feces of the broiler chickens in the coccidia group is obviously increased, and the average number of oocysts per gram of feces of the broiler chickens fed with the daily diet added with the examples and the comparative examples is obviously reduced, wherein the average number of oocysts per gram of feces of the examples 1 to 3 is obviously less than that of the comparative examples 1 to 6, and is equivalent to the effect of the commercial coccidia vaccine of the comparative example 7, but the feed additive provided by the application can avoid vaccination.

[0087] Therefore, the feed additive for preventing and treating coccidia infection of broiler chickens provided by the examples of the application can significantly improve the growth performance of the broiler chickens, and can significantly reduce the average number of oocysts per gram of feces of the broiler chickens, which has important significance for the healthy development of the broiler chickens.

[0088] In summary, the examples of the application have the following advantages:

[0089] The feed additive for preventing and treating coccidia infection of broiler chickens provided by the examples of the application is composed of the following four components: maxcruevian yeast, ferulic acid esterase, aminopeptidase and glucanase.

[0090] Among them, the effects of the four components are as follows:

[0091] Unlike traditional yeast, maxcruevian yeast is a typical Crabtree-negative yeast. Maxcruevian yeast has the functions of regulating cellular immunity, adhesion, intestinal microecology, and antioxidant, anti-stress, etc., has strong acid and bile salt tolerance (can smoothly pass through the gastrointestinal tract to reach the small intestine to play a probiotic effect), and has the activities of resisting pathogenic bacteria and resisting fungal infection, and can produce many biological active substances with high added value in the metabolic process, such as oligosaccharides, oligonucleotides, oligopeptides, etc., can play a probiotic effect as a prebiotic, and can express ferulic acid esterase;

[0092] Ferulic acid esterase refers to an enzyme that can hydrolyze the ester bond in ferulic acid methyl ester, oligosaccharide ferulic acid ester and polysaccharide ferulic acid ester to free ferulic acid, which can cut the cross-linking between polysaccharide-polysaccharide and polysaccharide-lignin in the cell wall, which is beneficial to the degradation of polysaccharide and the release of lignin in the cell wall material, and ferulic acid as a functional substance has the effects of antibacterial and anti-inflammatory;

[0093] Aminopeptidase is a kind of exoprotease that can selectively remove amino acid residues from the N-terminal of protein and polypeptide to produce free amino acid, can hydrolyze protein deeply, remove the bitterness of protein hydrolysate and produce bioactive polypeptide;

[0094] Glucomylase can effectively decompose beta-glucan in plant cell wall, reduce the content of non-starch polysaccharide and its anti-nutritional factors, and improve the growth rate and feed conversion efficiency of livestock and poultry.

[0095] There is no related research on the application of four components of Kluyveromyces marxianus, ferulic acid esterase, aminopeptidase and glucomylase in the prevention and treatment of chicken coccidiosis in the prior art, and the present embodiment explores this combination on the basis of a large amount of work, which has obvious effect on the prevention and control of chicken coccidiosis, effectively improves the production index of broilers, improves the economic benefit, and reduces the use of anticoccidial drugs.

[0096] Finally, it should be noted that: the above examples are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing examples, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing examples, or make equivalent replacement for part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A feed additive for the control of coccidial infection in broiler chickens, characterized in that, The feed additive comprises the following components: Maxikrurvye yeast, ferulic acid esterase, aminopeptidase and glucanase; The mass percentage of Maxikrurvye yeast is 30-40%; The mass percentage of ferulic acid esterase is 15-28%; The mass percentage of aminopeptidase is 15-25%; The mass percentage of glucanase is 18-25%; The viable bacteria or enzyme activity per gram of feed additive is: The viable bacteria activity of Maxikrurvye yeast is 150-500 billion; The enzyme activity of ferulic acid esterase is 50-2000 U; The enzyme activity of aminopeptidase is 500-30000 U; The enzyme activity of glucanase is 1000-300000 U.

2. The feed additive for controlling chicken coccidiosis according to claim 1, characterized in that, The mass percentage of Maxikrurvye yeast is 35%; The mass percentage of ferulic acid esterase is 20%; The mass percentage of aminopeptidase is 20%; The mass percentage of glucanase is 25%.

3. The feed additive for controlling chicken coccidiosis according to claim 1, characterized in that, The viable bacteria activity of Maxikrurvye yeast is 200 billion; The enzyme activity of ferulic acid esterase is 100 U; The enzyme activity of aminopeptidase is 800 U; The enzyme activity of glucanase is 2000 U.

4. A compound feed, characterized in that, The feed additive comprises the following components: Maxikrurvye yeast, ferulic acid esterase, aminopeptidase and glucanase; 5. The composite feed according to claim 4, characterized in that, The addition amount of the feed additive is 420-550 g / t of the mass of the poultry daily ration.

6. The composite feed according to claim 5, characterized in that, The addition amount of the feed additive is 500 g / t of the mass of the poultry daily ration.

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