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Method for preparing asparagus active polysaccharide

A technology of active polysaccharides and asparagus, which is applied in the field of preparation of asparagus polysaccharides, can solve the problems of low polysaccharide yield and poor biological activity

Inactive Publication Date: 2008-01-09
SHANGHAI FISHERIES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a preparation method of active polysaccharides from asparagus to solve the defects of low polysaccharide yield and poor biological activity in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] ①Pre-treatment: Rinse asparagus, dry in an oven at 45°C, pulverize with 80 mesh, and set aside;

[0020] ②Extraction of polysaccharides: Add 40 times the weight of water to the crushed asparagus powder, extract in a water bath at 50°C for 4 hours; centrifuge at 12000g for 20 minutes, repeat the above steps for centrifugal precipitation, extract once more, and combine filtrate;

[0021] ③Remove alginate: freeze the above filtrate at -20°C overnight, then melt at 4°C, centrifuge at 12000g for 30 minutes, and collect the supernatant;

[0022] ④ Dialysis of the extract: Concentrate the above supernatant, dialyze with a dialysis membrane at 4°C, the molecular cut-off of the dialysis membrane is 10,000; dialysis for 4-5 days, take the part with a molecular weight above 10,000;

[0023] ⑤ Drying: Freeze-drying the above-mentioned part with a molecular weight above 10,000 to obtain the desired polysaccharide from Asparagus.

[0024] The content of the prepared polysaccharide ...

Embodiment 2

[0026] ①Pre-treatment: Dried asparagus in an oven at 50°C, crushed to 80 mesh, and set aside;

[0027] ②Extraction of polysaccharides: Add 40 times the weight of water to the crushed asparagus powder, extract in a water bath at 60°C for 4 hours; centrifuge at 12000g for 20 minutes, repeat the above steps for centrifugal precipitation, extract once more, and combine filtrate;

[0028] ③Remove alginate: freeze the above filtrate at -20°C overnight, then melt at 4°C, centrifuge at 12000g for 30 minutes, and collect the supernatant;

[0029] ④ Dialysis of the extract: Concentrate the above supernatant, dialyze with a dialysis membrane at 4°C, the molecular cut-off of the dialysis membrane is 10,000; dialysis for 4-5 days, take the part with a molecular weight above 10,000;

[0030] ⑤ Drying: Freeze-drying the above-mentioned part with a molecular weight above 10,000 to obtain the desired polysaccharide from Asparagus.

[0031] The content of the prepared polysaccharide is 86.5%,...

Embodiment 3

[0033] The other steps are the same as in Example 1-2, except that asparagus powder is extracted in a water bath at 70°C, the prepared polysaccharide content is 80%, and the mouse lymphocyte proliferation rate is 212%.

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PUM

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Abstract

Preparation of agar active polysaccharide is carried out by: pre-treating, extracting for polysaccharide at optimum temperature, removing glue, dialyzing, and drying to obtain final product. The agar active polysaccharide recovery rate reaches to 13%, and polysaccharide content surpasses 76%. It has better breeding effect for mouse lymphocyte and immune activity.

Description

technical field [0001] The invention relates to the field of extraction of marine plant polysaccharides, in particular to a preparation method of asparagus polysaccharides. Background technique [0002] Asparagus is rich in seaweed polysaccharides, iodine, calcium, iron and other essential elements, trace elements and vitamins A, B1, C, etc. "Diet Therapy Materia Medica" and "Compendium of Materia Medica" record that asparagus has the effects of treating goiter, edema, uropathy, and preventing ulcers. Japanese studies have proved that asparagus has the effects of clearing the lungs and laxative, beautifying and slimming, lowering blood pressure and blood lipids, and regulating body functions. It is believed that eating asparagus is the main reason why the average life expectancy of Okinawa people in Japan is the highest in the world. [0003] But at present, the utilization of asparagus is also mainly used to produce agar and a small amount as abalone feed. Only the colloid...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 潘迎捷朱地琴唐庆九张劲松赵勇
Owner SHANGHAI FISHERIES UNIV
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