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Bioactive polypeptide YFGSGFAAPFFIVRHQLLKK and preparation method and use thereof

A bioactive peptide, bioactive peptide technology, applied in the protein field, to promote lymphocyte proliferation, improve the ability, and reduce the body's morbidity.

Active Publication Date: 2021-03-16
ZHEJIANG PANDA DAIRY GRP COMPANY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the related functions of Cytochrome oxidase subunit 7C, mitochondrial protein polypeptide fragments in the prior art

Method used

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  • Bioactive polypeptide YFGSGFAAPFFIVRHQLLKK and preparation method and use thereof
  • Bioactive polypeptide YFGSGFAAPFFIVRHQLLKK and preparation method and use thereof
  • Bioactive polypeptide YFGSGFAAPFFIVRHQLLKK and preparation method and use thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The artificial synthesis of embodiment 1 active peptide YFGSGFAAPFFIVRHQLLKK

[0034] 1. Synthesis of Bioactive Peptides

[0035] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak with 50ml of dichloromethane (DCM).

[0036] 2. After 2 hours, wash the resin with nitrogen-dimethylformamide (DMF) that is 3 times the volume of the resin, and then drain it. Repeat this four times, and drain the resin for use.

[0037] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0038] 4. Take a small amount of resin and test it with ninhydrin (Ninhydrin Nine Wells) method (two drops each of test A and test B, react at 100°C for 1 min), if the resin has color, it means that the deprotection is successful.

[0039] ...

Embodiment 2

[0076] The immune activity experiment of embodiment 2 bioactive peptides

[0077] 1. In vitro lymphocyte proliferation ability experiment of biologically active polypeptide YFGSGFAAPFFIVRHQLLKK (MTT method)

[0078] 1. Experimental materials and instruments:

[0079] Reagents and materials: experimental animals balb / c mice (male 6-8 weeks old, Animal Experiment Center, School of Agriculture and Biology, Shanghai Jiaotong University); the mouse spleen lymphocyte-derived bioactive peptide YFGSGFAAPFFIVRHQLLKK obtained in Example 1; mouse lymphocytes Cell extract (purchased from Suo Laibao); RPMI1640 medium (purchased from GIBCO); 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide ( MTT, purchased from Amresco); concanavalin (ConA, purchased from Sigma); bovine serum albumin (BSA, purchased from Genebase); pepsin (purchased from Sigma); from AB Company).

[0080] Equipment: LRH-250F biochemical incubator, Shanghai Heng Technology Co., Ltd.; GL-22M high-speed refrigerat...

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Abstract

The invention relates to the field of protein, in particular to a bioactive polypeptide YFGSGFAAPFFIVRHQLLKK and a preparation method and use thereof, and an amino acid sequence of the bioactive polypeptide YFGSGFAAPFFIVRHQLLKK is Tyr-Phe-Gly-Ser-Gly-Phe-Ala-Ala-Pro-Phe-Phe-Ile-Val-Arg-His-Gln-Leu-Leu-Lys-Lys. An in-vitro immune function regulation experiment proves that the polypeptide YFGSGFAAPFFIVRHQLLKK has a relatively good immune regulation function. The bioactive peptide YFGSGFAAPFFIVRHQLLKK can promote an increase of the induction amount of nitric oxide in macrophages, improves the capability of resisting an external pathogen infection of organisms, reduces a morbidity of the organisms, remarkably promotes lymphocyte proliferation of mice, improves the quality of life, and has veryimportant significance for developing food, health-care products and medicines with immunoregulation functions.

Description

technical field [0001] The present invention relates to the protein field, in particular to a biologically active polypeptide YFGSGFAAPFFFIVRHQLLKK and its preparation method and application. Background technique [0002] In recent years, bioactive peptides have become a familiar term. Because of its many potential biological functions, it has attracted more and more attention and has become one of the hot spots of scientific research. At present, the beneficial effects of many bioactive peptides have also been well proven, such as anti-cancer, lowering blood pressure, antibacterial, lowering cholesterol, anti-diabetic and other properties. More than 3000 different bioactive peptides have been reported in the current most authoritative bioactive peptide database BIOPEP-UMW. [0003] At present, most studies on bioactive peptides focus on food-derived peptides, while there are few studies and reports on non-food-derived peptides. And it has been confirmed by research resul...

Claims

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Application Information

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IPC IPC(8): C12N9/02A61K8/66A61Q19/00A61K38/44A61P29/00A61P37/02A23L33/18
CPCC12N9/0053A61K8/66A61Q19/00A61P29/00A61P37/02C12Y109/03001A23L33/18A61K38/00A23V2002/00A23V2200/324
Inventor 张少辉郭婷婷栾媛媛李锡安占文静陈承余肖珊珊石毅梁世排梁世赞
Owner ZHEJIANG PANDA DAIRY GRP COMPANY
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