Preparation method of Yupingfeng herb residue polysaccharide synbiotic and application of synbiotic in immunoregulation
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A technology of Yupingfeng and synbiotics, which is applied in the fields of application, drug combination, food preparation, etc., and can solve unseen problems
Active Publication Date: 2015-12-16
SICHUAN AGRI UNIV
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However, there are few reports of polysaccharide synbiotics fro
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Embodiment 1
[0020] The identification of embodiment 1 Rhizopus oligospore SH
[0021] The genome total DNA of mold SH was extracted according to the improved benzyl chloride method of (ZhuH, 1993. Nucleic Acids Research, 21 (22): 5279), and the total DNA was detected by 2% agarose gel ( figure 1 ). The ITS1-5.8S-ITS2 region of the total DNA of the mold SH genome was amplified according to the method of (Liu Xiaoli. 2009. Modern Food Science and Technology, 25 (5): 2578-2585), and the PCR product was detected with 1.0% agarose gel ( figure 2 ). The purified PCR product was sent to Beijing BGI for sequencing, and the sequence length of the mold SHITS1-5.8S-ITS2 region was determined to be 651bp (sequence table 1). After carrying out the Blast comparison in Genbank, it has the highest affinity with Rhizopus oligosporum, and the homology reaches 100%, and a phylogenetic tree is established ( image 3 ). Therefore, the present invention identified it as Rhizopus oligosporus (Rhizopus olig...
Embodiment 2
[0022] The domestication of embodiment 2 Rhizopus oligospore SH
[0023] Inoculate Rhizopus oligosporus SH into PDA containing 5% YPD, culture at 28°C for 24 hours, pass for 5 generations, and then inoculate into PDA culture solution containing 10%, 20%, 40%, 80%, and 100% YPD in sequence Cultured in medium for 24 hours, each concentration was continuously passed for 3 generations, and Rhizopus oligospora SH adapted to YPD was obtained, which was stored for future use.
Embodiment 3
[0024] Example 3 Preparation of polysaccharide synbiotics from Yupingfeng dregs
[0025] Dissolve YPD in distilled water (final concentration: 0.1g / mL), sterilize with flowing steam for 60min, cool down, inoculate purified and domesticated Rhizopus oligospora SH with 3% inoculum size, 150rpm, 28°C constant temperature fermentation for 2-3 days , Vacuum freeze-drying into powder is synbiotics. Synbiotics were taken to make a solution with a final concentration of 1g / mL, sterilized by a 0.22μm syringe filter, and stored at 4°C for later use.
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Abstract
The invention discloses a preparation method of Yupingfeng herb residue polysaccharide synbiotic and application of the synbiotic in immunoregulation and belongs to the field of traditional Chinese medicine residue recycling technology and microecologics. The synbiotic is prepared by using rhizopus oligosporus SH to ferment Yupingfeng herb residue polysaccharide (YPD). The preparation method is characterized by taking YPD as a unique raw material to obtain the Yupingfeng herb residue polysaccharide synbiotic through rhizopus oligosporus SH fermentation and including the links of dissolving of YPD, circulating steam sterilization, cooling, inoculation, fermentation and freeze-drying. By the synbiotic, in-vitro proliferation activity, phagocytic activity and NO releasing capability of rice lymphocytes and macrophagocytes are improved remarkably, and expression of IL-6, TNF-alpha, IFN-gamma, NF-kB, TLR-4 and iNOS in cells can be increased; in an LPS-induced chronic inflammation model, the synbiotic remarkably inhibits in-vitro proliferation activity of lymphocytes and remarkably decreases expression of IL-6, TNF-alpha, IFN-gamma, NF-kB, TLR-4 and iNOS in cells. Consequently, the Yupingfeng herb residue polysaccharide synbiotic has bidirectional immunoregulation effect and can serve as an immunoregulator.
Description
technical field [0001] The invention relates to the preparation of polysaccharide synbiotics from Yupingfeng medicinal dregs and its application in immune regulation, and belongs to the field of resource utilization technology of traditional Chinese medicine dregs and microecological preparations. Background technique [0002] Traditional Chinese medicine has been widely used in the prevention and treatment of various diseases due to its low toxicity and side effects, and has a long history in Asian countries. In recent years, both commercial demand and academic research, traditional Chinese medicine has received widespread attention, and the syndrome differentiation theory of traditional Chinese medicine has gradually gained popularity. A large number of studies have reported that many traditional Chinese medicines have immunomodulatory functions, but there are few studies on traditional Chinese medicine residues. With the rapid development of the Chinese medicine industry...
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