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Controlled live tumor cell vaccine and preparation method and application thereof

A tumor cell vaccine and live cell technology, applied in botany equipment and methods, biochemical equipment and methods, anti-tumor drugs, etc., can solve incomplete antigen presentation, low antigen presentation efficiency, poor effect of monovalent vaccines, etc. problems, to achieve the effects of overcoming immune escape, strong immune recognition and killing effects, and enhancing specific immune responses

Inactive Publication Date: 2009-10-21
GUANGZHOU GENERAL HOSPITAL OF GUANGZHOU MILITARY COMMAND
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  • Claims
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AI Technical Summary

Problems solved by technology

[0009] The first purpose of the present invention is to overcome the defects of low antigen presentation efficiency, incomplete antigen presentation, poor effect and low titer of monovalent vaccines in existing tumor cell vaccines, and to provide a controllable tumor cell vaccine vaccine

Method used

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  • Controlled live tumor cell vaccine and preparation method and application thereof
  • Controlled live tumor cell vaccine and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0045] This embodiment provides a controllable tumor live cell vaccine, which is characterized by:

[0046] 1) The suicide gene thymidine kinase TK and the immune regulation gene are transfected into tumor cells for transgenic operation to form a double gene modified controllable tumor living cell vaccine;

[0047] 2) The host can be vaccinated directly or fused with antigen-presenting cell DC, and the tumor-controllable live cell vaccine can be administered to inactivate GCV in vivo one week after inoculation;

[0048] 3) It can not only control the growth of the live vaccine in vivo, but also make the target gene fully secreted and expressed.

[0049] The preparation method of the present embodiment comprises the steps:

[0050] 1. Establishment of tumor cell lines stably expressing TK gene

[0051] In this example, the glioma cell line U87 is taken as an example for illustration, and the above operations are all performed under sterile conditions.

[0052] (1) In vitro c...

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Abstract

The invention relates to a controlled live tumor cell vaccine and a preparation method and application thereof. The preparation method is characterized in that a suicide gene thymidine kinase TK and an immunoregulatory gene are allowed to transfect tumor cells by slow virus infection and liposome mediation to perform transgene, cells stably expressing the target gene are screened for cloning culture and expansion to obtain a double-gene modified tumor cell vaccine. The controlled live tumor cell vaccine can help activate lymphopoiesis of an immune system, generate a specific CTL killing efficacy and / or a non-specific NK killing efficacy and generate significantly enhanced anti-tumor immune response in a host. The controlled live tumor cell vaccine has higher titer, more complete tumor antigen presentation, and increased and stable expression of exogenous immunoregulatory genes, and better transgene efficacy, thus enhancing specific immune reaction induced by the tumor cell vaccine and increasing efficacy of the tumor cell vaccine.

Description

technical field [0001] The invention relates to a controllable tumor living cell vaccine and its preparation method and application, belonging to the fields of tumor biological immunotherapy and cellular immunology. Background technique [0002] At present, cancer is still the main cause of death of human beings. Conventional methods of cancer treatment, such as surgery, radiotherapy and chemotherapy, are effective in treating some benign and slow-growing tumors, but for malignant solid tumors with infiltrating growth and rapid proliferation, these conventional treatment measures are not effective. ideal. [0003] A large number of studies have shown that the main reason why tumor cells can grow and proliferate out of control in the body is the defect or imperfection of the body's anti-tumor immune mechanism, so that tumor cells escape the body's "immune surveillance" and are in a state of "immune escape". This is especially reflected in the inability of the body's immune ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61P35/00C12N5/10C12N15/09C12N15/86C12N15/861
Inventor 王伟民林健戴学军
Owner GUANGZHOU GENERAL HOSPITAL OF GUANGZHOU MILITARY COMMAND
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