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Preparation of gene immunization treating tumor used for vein

An immunotherapy and gene technology, applied in gene therapy, medical preparations containing active ingredients, anti-tumor drugs, etc., can solve problems such as limitations and achieve high transfection efficiency

Inactive Publication Date: 2011-01-12
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This greatly limits the role of hTERTC27; at the same time, the stable transfection of hTERTC27 HeLa cells subcutaneously planted in nude mice has certain differences from natural tumor formation

Method used

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  • Preparation of gene immunization treating tumor used for vein
  • Preparation of gene immunization treating tumor used for vein
  • Preparation of gene immunization treating tumor used for vein

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Embodiment Construction

[0019] An intravenous preparation for gene immunotherapy for tumors consists of a replication-deficient adenovirus loaded with human telomerase reverse transcriptase C-terminal polypeptide gene hTERTC27 and acceptable pharmaceutical auxiliary materials. Acceptable pharmaceutical excipients are well known to those skilled in the art, such as normal saline and the like.

[0020] Preferably, the replication-defective adenovirus is Ad5.

[0021] The replication-deficient adenovirus Ad5 was co-transfected into 293 cells by the backbone plasmid pBHGlox_E1, 3Cre and the shuttle plasmid pDC316-hTERTC27-EGFP double plasmid, and homologous recombination produced recombinant adenovirus.

[0022] Below in conjunction with embodiment, further illustrate the present invention.

[0023] Preparation and identification of rAdv-hTERTC27 (recombinant adenovirus-hTERTC27-EGFP):

[0024] 1) Seed 293 cells in a six-well plate, 5×10 per well 5 cells, the medium was DMEM+10% Hyclone fetal bovine s...

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Abstract

The invention discloses a preparation of gene immunization treating tumor used for veins, which is composed of replication deficient adenovirus loading human telomerase reverse transcriptase C-terminal polypeptide hTERTC27 and an acceptable pharmaceutic adjuvant. The anti-tumor gene preparation rAdv-hTERTC27 of the invention has high transfection efficiency, and is more effective on the increment and the apoptosis regulation of the tumor cell. The anti-tumor gene preparation of the invention can effectively lead the peptides transfection to enter into the dendritic cell DCs, so as to activate the DCs, secrete IFN-gamma, IL-2, increase and further stimulate T lymphopoiesis, promote the T lymphocyte to generate the special killing effect CTL corresponding to the tumor cell, and immunoregulation is carried out on the tumor.

Description

technical field [0001] The invention relates to a gene preparation, in particular to an intravenous preparation for gene immunotherapy for tumors. Background technique [0002] hTERTC27 (C-terminal fragment of the human telomerase reverse transcriptase, hTERTC27), is a 27kD human telomerase reverse transcriptase C-terminal polypeptide, which can cause abnormal function of telomeres, allowing the rapid fusion of chromosome ends without affecting telomeres Enzyme activity that leads to hTERT + Apoptosis and aging of HeLa cells produced obvious tumor-suppressive effect on the nude mouse model of HeLa cells (Huang, J. J., Lin, M. C., Bai YX, Jing D, Wong BC, Han SW, Lin J, Xu B, Huang CF, Kung HF. Ectopic expression of a COOH-terminal fragment of the human telomerase reverse transcriptase leads to telomere dysfunction and reduction of growth and tumorigenicity in HeLa cells. Cancer Research. 62:3226-3232, 2002.;. Huang, J. J. , Han SW, Bai YX, Ng SSM, Jing DD, Wong BCY, Huang...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K31/711A61P35/00
Inventor 彭英何蕾龚汉贤黄君健孔祥复
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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