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Ganoderma lucidum polysaccharide and preparation method thereof

A technology of Ganoderma lucidum polysaccharide and Ganoderma lucidum, applied in the field of Ganoderma lucidum polysaccharide and its preparation, can solve the problems of many impurities, dark color, etc., and achieve the effects of simple preparation method, improved immune activity, and stimulation of proliferation

Inactive Publication Date: 2011-01-05
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

With the discovery of more and more biological activities of Ganoderma lucidum polysaccharides in recent years, there are more and more products of Ganoderma lucidum polysaccharides. However, the products of Ganoderma lucidum polysaccharides extracted by the traditional water extraction process have dark color and many impurities, which are not conducive to being used as functional foods. or pharmaceuticals for further research and development

Method used

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  • Ganoderma lucidum polysaccharide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Preparation of ganoderma lucidum crude polysaccharide by water extraction and ultrafiltration

[0024] Crush the fruiting body of Ganoderma lucidum into small pieces, add water according to the ratio of 1kg fruiting body to 20L water, heat and boil for 2 hours, then filter with a filter cloth (100 mesh), repeat the above steps to extract the filter residue once more and filter, combine the two Extracted filtrate. After the filtrate is concentrated to 10L, the concentrated solution is transferred to UF-3530 ultrafiltration membrane for ultrafiltration (ultrafiltration membrane with a molecular weight cut-off of 10,000 and 500,000 Da is selected), and freeze-dried to finally obtain Ganoderma lucidum crude oil of 10,000-500,000 Da. Dry polysaccharides.

Embodiment 2

[0026] 1. Starting material preparation

[0027] The 1-500,000 Da Ganoderma lucidum crude polysaccharide dry product of Example 1 was formulated into a solution at a mass ratio of 15%, and the precipitated part was removed by centrifugation.

[0028] 2. Anion exchange resin pretreatment

[0029] Rinse with a small flow of 4% HCl until the effluent is acidic, soak for 3 hours, then drain the acid solution, rinse with deionized water until neutral; rinse with a small flow of 4% NaOH until the effluent is alkaline, soak 6h, then drain the lye, rinse with deionized water until neutral.

[0030] 1-500,000 Da ganoderma crude polysaccharide aqueous solution was added to the pretreated anion exchange resin (addition ratio: 40mL ganoderma crude polysaccharide aqueous solution was added to 5mL resin), placed in a 250mL Erlenmeyer flask, shaken in a shaker, 120rpm / min, Decolorize at 30°C and pH 5.0 for 3 hours, then filter with a filter cloth (100 mesh), collect the decolorized Ganoder...

Embodiment 3

[0038] 1. Starting material preparation

[0039] The 1-500,000 Da Ganoderma lucidum crude polysaccharide dry product of Example 1 was formulated into a solution at a mass ratio of 40%, and the precipitated part was removed by centrifugation.

[0040] 2. Anion exchange resin treatment (resin pretreatment method is the same as embodiment 2)

[0041] 1-500,000 Da Ganoderma lucidum crude polysaccharide aqueous solution was added to the pretreated anion exchange resin (addition ratio: 40mL Ganoderma lucidum polysaccharide crude extract was added to 10mL resin; flow rate 3BV / h; room temperature; sample volume 40mL) for decolorization in the column, and collected Decolorized crude polysaccharide aqueous solution.

[0042] Measure its polysaccharide content and pigment, compare with embodiment 1, calculate and obtain its decolorization rate 91.06%, polysaccharide retention rate 88.34%.

[0043] 3. Freeze-drying (same as Example 2) to obtain Ganoderma lucidum polysaccharide.

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Abstract

The invention provides ganoderma lucidum polysaccharide and a preparation method thereof. The ganoderma lucidum polysaccharide is obtained through carrying out water extraction and ultrafiltration on a ganoderma lucidum fruiting body, decoloring and then freeze drying. The decolored ganoderma lucidum polysaccharide has remarkably improved immunocompetence and can stimulate macrophage to generate nitric oxide and stimulate lymphopoiesis.

Description

technical field [0001] The invention relates to the field of edible fungi, in particular to a ganoderma polysaccharide and a preparation method thereof. Background technique [0002] Ganoderma lucidum, also known as fairy grass and god grass, is a precious medicinal fungus that strengthens the body, nourishes and strengthens. Ganoderma lucidum polysaccharide is one of the most important active ingredients of Ganoderma lucidum. Modern research shows that Ganoderma lucidum polysaccharides have anti-thrombosis, anti-tumor, anti-radiation, anti-oxidation, immune regulation, liver protection, and antihypertensive effects. With the discovery of more and more biological activities of Ganoderma lucidum polysaccharides in recent years, there are more and more products of Ganoderma lucidum polysaccharides. However, the products of Ganoderma lucidum polysaccharides extracted by the traditional water extraction process have dark color and many impurities, which are not conducive to bein...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P1/16A61P7/02A61P9/12A61P35/00A61P37/02A61P39/06
Inventor 张劲松罗玺唐庆九周帅刘艳芳杨焱贾薇唐传红吴迪冯娜
Owner SHANGHAI ACAD OF AGRI SCI
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