Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Deoxycholate hydrogen sulfide lactose agar medium and application thereof

A technology of bile sulphur milk agar and culture medium, which is applied in the field of microorganisms, can solve the problems of contamination of food, poisoning, diseases caused by animals such as livestock, rodents and poultry, etc., and achieves low mutation rate, convenient preparation and operation, and low cost. low effect

Inactive Publication Date: 2015-01-21
QINGDAO KANGHEWEIYE COMML
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Except typhoid bacillus, paratyphoid A and paratyphoid bacillus causing human diseases, most of them can only cause animal diseases such as livestock, rodents and poultry, but sometimes they can also contaminate human food and cause food poisoning

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of gallbladder milk agar culture medium, the component of described culture medium comprises 20.1g / LGN enrichment liquid, 8.0g / L lactose, 2.8g / L sodium thiosulfate, 1.5g / L sodium citrate, 1.5g / L ferric ammonium citrate, 0.023g / L neutral red, 16.5g / L agar, pH value is 7.1-7.3.

[0020] The GN enrichment liquid contains tryptone 20.0g, glucose 2.0g, dipotassium hydrogen phosphate 1.5g, mannitol 3g, sodium citrate 5.8g, sodium deoxycholate 0.33g, Sodium chloride 5.0g, dipotassium hydrogen phosphate 2.0g.

[0021] Peptone and glucose provide carbon sources, nitrogen sources, vitamins and minerals; lactose is a fermentable sugar; sodium deoxycholate and sodium citrate inhibit Gram-positive bacteria and most coliforms and Proteus, but Does not affect the growth of Salmonella; sodium thiosulfate and ferric ammonium citrate are used to detect the production of hydrogen sulfide, making the center of the colony black; neutral red is a pH indicator, the colonies that ferm...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of microorganisms and in particular relates to a deoxycholate hydrogen sulfide lactose agar medium. The medium comprises the following components: 20.1g / L of GN enrichment fluid, 8.0g / L of lactose, 2.8g / L of sodium thiosulfate, 1.5g / L of sodium citrate, 1.5g / L of ammonium ferric citrate, 0.023g / L of neutral red and 16.5g / L of agar, wherein the pH value of the medium is 7.1-7.3; the GN enrichment fluid per liter contains 20.0g of tryptone, 2.0g of glucose, 1.5g of dipotassium hydrogen phosphate, 3g of mannitol, 5.8g of sodium citrate, 0.33g of deoxysodium cholate, 5.0g of sodium chloride and 2.0g of dipotassium hydrogen phosphate. The deoxycholate hydrogen sulfide lactose agar medium has the beneficial effects that the medium is simple in components, convenient in preparation operation and low in cost and is beneficial to popularization and application; the bacteria grow rapidly and the mutation rate is low; the medium is convenient to observe and analyze.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a gallic acid milk agar medium and an application thereof. Background technique [0002] The causative agent of salmonellosis. Enterobacteriaceae, Gram-negative enterobacteriaceae. Nearly a thousand species (or strains) have been discovered. According to its antigenic composition, Salmonella can be divided into basic bacterial groups such as A, B, C, D, and E. Among them, those related to human diseases mainly include A. paratyphi in Group A, B. paratyphi and Bacillus typhimurium in group B, C. paratyphi and Bacillus choleraesuis in group C, typhoid bacillus and Enteritidis bacteria in group D, etc. In addition to typhoid bacillus, paratyphoid A and paratyphoid bacillus causing human diseases, most of them can only cause animal diseases such as livestock, rodents and poultry, but sometimes they can also contaminate human food and cause food poisoning. Salmonella is n...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/42
CPCC12N1/20
Inventor 高玉成刘永祥
Owner QINGDAO KANGHEWEIYE COMML
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com