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Divaricate velvetplant root and rhizome polysaccharide and application thereof in preparing medicine and functional food for immune adjustment and tumor resistance

A technology of Panax notoginseng and functional food, applied in the field of medicine, can solve the problems of immune regulation and antitumor activity of Panax notoginseng polysaccharide, which have not been reported, and achieve good market application prospects, improve the level of white blood cells, and inhibit migration. Effect

Active Publication Date: 2015-11-04
MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Panax notoginseng polysaccharide is an important component of it. In recent years, studies have found that these two compounds have antidiabetic activity and blood pressure lowering effect, but there is no report on the immune regulation and antitumor activity of Panax notoginseng polysaccharide

Method used

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  • Divaricate velvetplant root and rhizome polysaccharide and application thereof in preparing medicine and functional food for immune adjustment and tumor resistance
  • Divaricate velvetplant root and rhizome polysaccharide and application thereof in preparing medicine and functional food for immune adjustment and tumor resistance
  • Divaricate velvetplant root and rhizome polysaccharide and application thereof in preparing medicine and functional food for immune adjustment and tumor resistance

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1, Extraction, Preparation and Analysis of Panax notoginseng Polysaccharide

[0026] The preparation method of Panax notoginseng polysaccharide is as follows: take 50 g of Panax notoginseng stems and leaves, dry and pulverize them, pass through a 50-mesh sieve to obtain Panax notoginseng powder, add 20 times the mass of deionized water, and extract by reflux at 70-100°C for 2 hours. Centrifuge, take the supernatant, and add 5 times the mass of deionized water to the precipitate, reflux at 70-100°C for 2 hours, centrifuge, and combine the filtrate; add a certain amount of 95% ethanol to the combined filtrate to make the final ethanol concentration 75%, alcohol precipitation overnight, centrifugation to remove the supernatant, the precipitate is deproteinized and decolorized, and then freeze-dried, and the finally obtained biologically active polysaccharide is the Panax notoginseng polysaccharide of the present invention.

[0027] According to HPLC molecular weigh...

Embodiment 2

[0028] Example 2, the promoting effect of Panax notoginseng polysaccharide on the phagocytosis of neutral red in RAW264.7 cells

[0029] Mouse mononuclear macrophage RAW264.7 cells were cultured in DMEM high glucose medium containing 10% fetal bovine serum. Cells in the logarithmic growth phase were taken at 1.0×10 5 The concentration of cells / mL was inoculated in a 96-well plate in 5% CO 2 , 37°C, cultured in a constant temperature incubator with saturated humidity until the cells adhered to the wall (about 6-8h), adding different concentrations of Panax notoginseng polysaccharides (1, 5, 10, 25, 50, 100 μg / ml) for a total of 6 concentrations, the positive drug is lentinan, the final concentration is 2 μg / ml, set 4 parallel wells for each concentration, take it out after 12 hours of culture, absorb the original medium, wash it once with PBS at room temperature, add 100 μL of 0.075% medium to each well Neutral red staining solution, continue to incubate for 30 minutes, absor...

Embodiment 3

[0034] Example 3, the effect of Panax notoginseng polysaccharide on promoting the secretion of nitric oxide from RAW264.7 cells

[0035] Mouse mononuclear macrophage RAW264.7 cells were cultured in DMEM high glucose medium containing 10% fetal bovine serum. Cells in the logarithmic growth phase were inoculated in 96-well plates at a concentration of 1.0x105 cells / mL, cultured in a constant temperature incubator with 5% CO2, 37°C, and saturated humidity until the cells adhered to the wall (about 6-8 hours), and then added There are 6 concentrations of different concentrations of Panax notoginseng polysaccharide (1, 5, 10, 25, 50, 100 μg / ml), the positive drug is lentinan, the final concentration is 2 μg / ml, and 4 parallel wells are set for each concentration. Take it out after 24 hours of cultivation, take 50 μL of the culture base into another 96-well plate, and detect it according to the instruction of the nitric oxide detection kit (Beiyuntian Bioreagent Company), that is, t...

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Abstract

The invention provides divaricate velvetplant root and rhizome polysaccharide and application thereof in preparing a medicine and functional food for immune adjustment and tumor resistance. According to the invention, the prepared divaricate velvetplant root and rhizome polysaccharide can promote mononuclear macrophage strain to phagocytose neutral red and release nitric oxide, can promote B cell proliferation and T cell proliferation, can also obviously improve immunosuppressive mouse spleen and thymus indexes, can improve the transformation and the proliferation of T cells and B cells, can increase the level of immunosuppressive mouse white blood cells, can obviously restrain the growth of tumor tissues, can protect organisms, can restrain the decline of weight, can gain weight, and can obviously restrain the migration of epidermic cells in veins. In addition, polysaccharide is the main functional component of divaricate velvetplant roots and rhizomes, has various health-care efficacies, is derived from purely natural materials, does not have any side effects, and can be developed into medicines and health-care products for immune adjustment, tumor resistance and angiogenesis resistance, so that the divaricate velvetplant root and rhizome polysaccharide has favorable market application prospects.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a polysaccharide of Panax notoginseng and its application in preparing medicines and functional foods for immunoregulation and antitumor. Background technique [0002] In recent years, low immune function, including primary and secondary immune function deficiency, deficiency or damage, has been widely concerned by researchers at home and abroad, because they themselves and the various complications induced, especially infection, often Make the patient's condition worse, difficult to treat, and can cause serious consequences, even life-threatening. Therefore, finding safe drugs to improve the body's immune function will save more patients, and has a good application prospect in the health care industry. [0003] In addition, tumor is a difficult topic in the medical field in the 21st century, and it is the disease with the highest mortality rate in the world. Findin...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A23L1/09A61P37/02A61P35/00
Inventor 郝杰杰管华诗周晓琳敦云楼于广利夏萱
Owner MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD
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