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Grain fatty acid value detection reagent, detection method and application of detection reagent

A technology for fatty acid value detection and detection reagents, which is applied in the direction of analysis by chemical reaction of materials, and material analysis by observing the impact on chemical indicators, which can solve problems such as difficulty, achieve convenient detection and save operation time. , the effect of simple operation

Inactive Publication Date: 2016-08-31
时树松
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the color of the indicator changes from light yellow to light green in the pH range of 5.0-6.4, which is often close to the background color of grain, it is not easy to use this indicator to sensitively indicate the fatty acid value of grain in actual use. Variety

Method used

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  • Grain fatty acid value detection reagent, detection method and application of detection reagent
  • Grain fatty acid value detection reagent, detection method and application of detection reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1) Weigh the following components by weight percentage: 0.04% of neutral red, 0.04% of bromothymol blue, 0.013% of sodium carbonate, and 25% of propylene glycol by volume, add water to constant volume, and mix well to prepare the detection reagent;

[0029] 2) if figure 1 As shown, spread the sample 1 to be tested on the white paper, adjust the nozzle 2 to be perpendicular to the plane of the sample to be tested, and about 2-5 cm away from the sample to be tested, press the button 3, so that the liquid medicine passes through the nozzle from the liquid storage bottle 4 2. Spray out and evenly spray onto the sample 1 to be tested;

[0030] 3) After reacting for 1 minute, observe the color change of the sample to be tested and compare it with the standard color plate to obtain the fatty acid value of the sample or make a judgment on the aging degree of the sample.

Embodiment 2

[0032] 1) Weigh 0.01% of neutral red, 0.01% of bromothymol blue, 0.005% of sodium carbonate, and 10% of propylene glycol by volume in the following weight percentages, add water to volume, and mix well to prepare the detection reagent;

[0033] 2) if figure 1 As shown, spread the sample 1 to be tested on the white paper, adjust the nozzle 2 to be perpendicular to the plane of the sample to be tested, and about 2-5 cm away from the sample to be tested, press the button 3, so that the liquid medicine passes through the nozzle from the liquid storage bottle 4 2. Spray out and evenly spray onto the sample 1 to be tested;

[0034] 3) After reacting for 3 minutes, observe the color change of the sample to be tested and compare it with the standard color plate to obtain the fatty acid value of the sample or make a judgment on the aging degree of the sample.

Embodiment 3

[0036] 1) Weigh 0.1% of neutral red, 0.1% of bromothymol blue, 0.05% of sodium carbonate, and 50% of propylene glycol by volume in the following weight percentages, add water to constant volume, and mix well to prepare the detection reagent;

[0037] 2) if figure 1 As shown, spread the sample 1 to be tested on the white paper, adjust the nozzle 2 to be perpendicular to the plane of the sample to be tested, and about 2-5 cm away from the sample to be tested, press the button 3, so that the liquid medicine passes through the nozzle from the liquid storage bottle 4 2. Spray out and evenly spray onto the sample 1 to be tested;

[0038] 3) After reacting for 3 minutes, observe the color change of the sample to be tested and compare it with the standard color plate to obtain the fatty acid value of the sample or make a judgment on the aging degree of the sample.

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Abstract

The invention provides a reagent which is capable of sensitively indicating the grain fatty acid value change only by means of visual colorimetric analysis and a detection method of the reagent, and the grain aging degree is indicated by the grain fatty acid value change. The detection reagent is prepared by the components, by weight, of 0.01%-0.1% of neutral red, 0.01%-0.1% of Bromothymol blue, 0.005%-0.05% of sodium carbonate, 10%-50% by volume of propylene glycol and the balance of water; the detection method comprises the steps that (1) appropriate components are weighed and dissolved, and the detection reagent is prepared; (2) a to-be-detected grain sample is placed on a piece of flat paper, the detection reagent is aimed at the grain sample and sprayed to the grain sample by one time, and the color change of the grain sample is observed within 1-10 minutes. According to the grain fatty acid value detection reagent, the detection method and application of the detection reagent, the manner of spraying the reagent to the surface of the grain sample is adopted, and the grain fatty acid value and the aging degree are detected by comparing the grain surface color change with a standard color card. Therefore, the method is easier to operate, the detection is more convenient, no other auxiliary equipment is required, and the operation time is saved.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a detection reagent, detection method and application of grain fatty acid value. Background technique [0002] Usually, the grains that are not suitable for direct use as rations are defined as "aged grains". The main detection indicators to detect whether the grain is "aged" are: fatty acid value, viscosity, taste score value and color and smell. Fatty acids in food are formed by the hydrolysis of fat under the action of lipase or acid-base. If the temperature is higher and the humidity is higher, the fatty acid value will also increase. Fatty acid value is a sensitive indicator to reflect changes in grain quality, and an important basis for judging grain quality. There is a good correlation between fatty acid value and storage quality, and it has been used as an important indicator of grain storage quality. For example, my country's "Rules for Judgment of Ri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 时树松
Owner 时树松
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