Flue gas condensate cytotoxicity determination method based on cell electronic sensor

An electronic sensor and flue gas condensation technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., to achieve high sensitivity, good repeatability, and accurate operation
CN102242181BActive Publication Date: 2013-02-13ZHENGZHOU TOBACCO RES INST OF CNTC
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHENGZHOU TOBACCO RES INST OF CNTC
Publication Date
2013-02-13

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Abstract

A flue gas condensate cytotoxicity determination method based on a cell electronic sensor is characterized by comprising the following technology steps:1) preparation of experiment reagents; 2) preparation of a flue gas condensate; 3) cell inoculation culture; 4) flue gas condensate contamination; 5) results and analysis. The invention utilizes a cell sensor chip to monitor cell growth conditionsand influences of different condensate concentrations on cell growth after contamination of cells by a cigarette mainstream flue gas condensate on a real time basis, so as to establish a new, real-time, mark-free and high-flux method for cigarette mainstream flue gas cytotoxicity determination. Compared with a neutral red absorption method, the method of the invention omits a neutral red dyeing step and a complicated cleaning step to simplify a test process; meanwhile, quality control on cells is carried out according to a real-time cell growth curve after inoculation to optimize contamination time and contamination concentration, and monitor influences of different condensate concentrations on the cell growth curve after contamination of cells on a real time basis, so as to obtain a moreaccurate and reliable experiment result.
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Description

technical field

[0001] The invention relates to a technique for measuring the cytotoxicity of cigarette mainstream smoke condensate, in particular to a new method for measuring the cytotoxicity of smoke condensate based on a real-time, label-free, high-throughput cell sensor chip. Background technique

[0002] In vitro toxicology tests are of great significance for human health risk assessment, cigarette hazard assessment and toxicity determination of cigarette additive materials. Among them, the neutral red test, one of the methods recommended by CORESTA, is a widely accepted and feasible in vitro cytotoxicity test method. However, the neutral red test adopts the endpoint detection method, and the selection of the detection time point has a significant impact on the detection results. The influence of the neutral red dye itself on the state of the cells cannot be eliminated. In addition, neutral red dye staining, multiple washing steps increase the possibility of introduc...

Claims

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