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Microfluidic paper chip array, preparation method and application thereof

A microfluidic paper chip and paper chip technology, which is applied in the direction of analyzing materials through chemical reactions, and analyzing materials through observation of the influence of chemical indicators, to achieve accurate results, promote applications, and high detection sensitivity.

Active Publication Date: 2018-12-07
JIANGSU HENGSHUN VINEGAR IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

In this new technology, there are several technical benefits that make its usage better than previous methods like traditional analytics or multidimensional techniques such as XRF (X Ray Fluorescence). These improvements allow for improved accuracy compared to existing systems while also reducing complexity by utilizing multiple different indexes instead of just one.

Problems solved by technology

This patented technical problem addressed in this patent relates to improving the taste, texture, odour, luster, appearance, moisture retention ability, cook ingrowth rate, shelf life span, productivity during production processes due to changing conditions inside muscle tissue caused by various factors like enzyme activity levels and biosynthesis rates. These variables affect how well these properties impact on consumer acceptance of processed safer vegetarian meavers called mutton bars. Additionally, there may exist concerns over potential harmful effects associated with excessive intake of certain types of animal feedstuffs containing too much free radicals. To address these challenges, research suggests developing methods to evaluate edible fats and oils based on their chemical composition and physical attributes.

Method used

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  • Microfluidic paper chip array, preparation method and application thereof
  • Microfluidic paper chip array, preparation method and application thereof
  • Microfluidic paper chip array, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Preparation of microfluidic paper chip array

[0052] 1) Use Microsoft Word 2010 software to design the shape of the paper chip. The total length of the flow channel is 36mm, the width is 2mm, and the radius of the circular paper chip at both ends of the channel is 2mm. Outlines were printed on Whatman No. 1 cellulose chromatography paper, and paper chips were cut out. like figure 1 shown. The paper chip is easy to process and has a complete shape. Five paper chips are prepared to form a microfluidic paper chip array.

[0053] 2) Preparation of reagents:

[0054] 0.001mol / L methyl red: Weigh 0.014g of methyl red into a 50mL beaker, add absolute ethanol to dissolve, and dilute to 50mL with water, set aside;

[0055] 0.001mol / L neutral red: Weigh 0.014g of neutral red into a 50mL beaker, add absolute ethanol to dissolve, and dilute to 50mL with distilled water, set aside;

[0056] 0.001mol / L Bromocresol Violet: Weigh 0.027g of Bromocresol Violet into a 50mL...

Embodiment 2

[0061] Example 2 Preparation of microfluidic paper chip array

[0062] 1) Use CAD2008 software to design the shape of the paper chip. The total length of the flow channel is 20 mm, the width is 1 mm, and the radius of the circular paper chip at both ends of the channel is 5 mm. Outlines were printed on Whatman No. 1 cellulose chromatography paper, and paper chips were cut out. The paper chip is easy to process and has a complete shape. Five paper chips are prepared to form a microfluidic paper chip array.

[0063] Preparation of reagents:

[0064] 0.001mol / L methyl red: Weigh 0.014g of methyl red into a 50mL beaker, add appropriate amount of absolute ethanol to dissolve, and dilute to 50mL with distilled water, set aside;

[0065] 0.001mol / L neutral red: Weigh 0.014g of neutral red into a 50mL beaker, add an appropriate amount of absolute ethanol to dissolve, and dilute to 50mL with distilled water for later use;

[0066] 0.001mol / L Bromocresol Violet: Weigh 0.027g of Bromo...

Embodiment 3

[0070] Embodiment 3 Obtaining method of matrix X

[0071] Take 2g of fresh pork, add 2mL of pure water, grind for 5min; grind into a homogenous slurry. Take the paper chip array prepared by the method in Example 2, take pictures with a camera, and perform white balance correction before taking pictures.

[0072] Slowly add 60 μL of the homogenate dropwise to the sample pool, and the liquid flows from the sample pool to the chromogenic pool along the channel. When the liquid enters the chromogenic pool 300s, take a picture.

[0073] Process the paper photos with the software Image-Pro Plus 6.0 to obtain the Δ in the area T R, Δ T G, Δ T B value (such as Figure 7 shown). Subtract the R, G, and B values ​​after detection from the R, G, and B values ​​before detection to obtain ΔR, ΔG, and ΔB, and finally obtain the sum of the changes in ΔR, ΔG, and ΔB values ​​​​of all pixels in the chromogenic pool area Δ T R, Δ T G, Δ T B is the matrix X, and the result is as follows: ...

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Abstract

The invention discloses a microfluidic paper chip array. The microfluidic paper chip array comprises a paper chip array composed of a plurality of independent paper chips; each independent paper chipcomprises a flow separation area, a sampling pond and a developing pond; the flow separation area is made of a single-channel paper chip; the sampling pond and the developing pond are made of circularpaper chips located on the two ends of the flow separation area; the developing pond is acquired by dropwise adding different color developing agents and then drying under room temperature; the colordeveloping agents are selected from one or more of methyl red, neutral red, bromcresol purple, zinc porphyrin and cobalt porphyrin. The invention also discloses a preparation method for the microfluidic paper chip array and an application thereof. The invention fully utilizes the characteristic of the paper chip having a separating function to reduce a separating link in routine analysis and overcome the defect of inaccuracy of single index detection. The microfluidic paper chip array is high in detection sensitivity, can achieve an accurate result and requires no advanced instrument and equipment, so that the microfluidic paper chip array is suitable for a majority of people to use.

Description

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Claims

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Application Information

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Owner JIANGSU HENGSHUN VINEGAR IND
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