Pepsinogen II detection kit and preparation method thereof

Abstract

The invention discloses a pepsinogen II detection kit, which comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from 50-150 mmol / L of sodium phosphate buffer solution, 2-3 g / Lof bovine serum albumin, methylisothiazolinone with the concentration of 2-3%, 1.0-5.0 g / L of fatty alcohol and ethylene oxide condensate, 3-5 mmol / L of magnesium chloride and 20-30 mmol / L of ammonium formate, and the balance of deionized water.; and the reagent R2 is prepared from 1.3-2 mg / ml of pepsinogen II antibody emulsion solution, 2-3 g / L of bovine serum albumin, methylisothiazolinone withthe concentration of 2-3%, and the balance of deionized water. The prepared pepsinogen II detection kit and the preparation method of the pepsinogen II detection kit have the advantages that the chyle problem can be effectively solved, the anti-interference capability is high, and meanwhile, the stability and the sensitivity are high.

Description

technical field

[0001] The invention relates to a pepsinogen II detection kit and a preparation method thereof. Background technique

[0002] Pepsinogen (pepsinogen), synthesized by the main cells of the oxyntic gland, becomes pepsin in the gastric cavity through the action of hydrochloric acid (HCL) or active pepsin, and decomposes protein into fat, peptone and a small amount of peptides , the level of serum pepsinogen reflects the morphology and function of gastric mucosa in different parts: PGI is a pointer to detect the function of gastric acid gland cells, gastric acid secretion increases, PGI increases, secretion decreases or gastric mucosal glands atrophy, PGI decreases; PGII and gastric fundus The correlation of mucosal lesions is greater (compared to gastric antrum mucosa), and its increase is related to fundic gland duct atrophy, gastric epithelial metaplasia or pseudopyloric gland metaplasia, and dysplasia; the progressive decrease of PGI / II ratio is related to ga...

Examples

Embodiment 1

[0019] A pepsinogen II detection kit, including R1 reagent and R2 reagent, the R1 reagent includes Good's buffer 100mmol / L, sodium chloride 80mmol / L, magnesium chloride 12mmol / L, α-glucosidase 3kU / L, Anti-saliva amylase monoclonal antibody 27mg / L, bovine serum albumin 3g / L, polyvinylpyrrolidone 50mg / L, N-acetylcysteine ​​50mmol / L, and the rest is distilled water; the R2 reagent includes Good's buffer 150mmol / L, 4,6-ethylene-α, D-malto7 glucoside-p-nitrophenol (EPS-G7) 1.6mmol / L, bovine serum albumin 4g / L, ascorbic acid oxidase 1200U / L, and the rest distilled water.

[0020] Reagent R1 reagent and R2 reagent preparation:

[0021] 1) Add bovine serum albumin, concentration of methylisothiazolinone, fatty alcohol and ethylene oxide condensate, magnesium chloride, ammonium formate and sodium phosphate buffer, vortex for 10min, and place at 4-5°C After 2h, centrifuge at 800r / min for 20min, then let it stand for 2 hours, take the supernatant at 3-5°C for later use, and prepare th...

Embodiment 2

[0032] A pepsinogen II detection kit, including R1 reagent and R2 reagent, the R1 reagent includes sodium phosphate buffer 100mmol / L, bovine serum albumin 2g / L, concentration is 3% methylisothiazolinone, fat Alcohol and ethylene oxide condensate 3g / L, magnesium chloride 4mmol / L, ammonium formate 25mmol / L, and the rest is deionized water; the R2 reagent includes pepsinogen II antibody emulsion solution 2mg / ml, bovine serum albumin 2g / L, the concentration is 3% methylisothiazolinone, and the rest is deionized water.

[0033] Reagent R1 reagent and R2 reagent preparation:

[0034] 1) Add bovine serum albumin, concentration of methylisothiazolinone, fatty alcohol and ethylene oxide condensate, magnesium chloride, ammonium formate and sodium phosphate buffer, vortex for 10min, and place at 5°C for 2h Centrifuge at 800r / min for 20min, let stand for 2 hours, take the supernatant at 5°C for later use, and prepare the R1 reagent;

[0035] 2) First, add bovine serum albumin, methylis...

Embodiment 3

[0044] A pepsinogen II detection kit, including R1 reagent and R2 reagent, said R1 reagent includes sodium phosphate buffer 100mmol / L, bovine serum albumin 3g / L, concentration is 2% methylisothiazolinone, fat Alcohol and ethylene oxide condensate 3g / L, magnesium chloride 3mmol / L, ammonium formate 30mmol / L, and the rest is deionized water; the R2 reagent includes pepsinogen II antibody emulsion solution 2mg / ml, bovine serum albumin 2g / L, the concentration is 3% methylisothiazolinone, and the rest is deionized water.

[0045] Reagent R1 reagent and R2 reagent preparation:

[0046] 1) Add bovine serum albumin, concentration of methylisothiazolinone, fatty alcohol and ethylene oxide condensate, magnesium chloride, ammonium formate and sodium phosphate buffer, vortex for 10min, and place at 5°C for 2h Centrifuge at 800r / min for 20min, let stand for 2 hours, take the supernatant at 5°C for later use, and prepare the R1 reagent;

[0047] 2) First, add bovine serum albumin 2-3g / L, ...

technical field

[0001] The invention relates to a pepsinogen II detection kit and a preparation method thereof. Background technique

[0002] Pepsinogen (pepsinogen), synthesized by the main cells of the oxyntic gland, becomes pepsin in the gastric cavity through the action of hydrochloric acid (HCL) or active pepsin, and decomposes protein into fat, peptone and a small amount of peptides , the level of serum pepsinogen reflects the morphology and function of gastric mucosa in different parts: PGI is a pointer to detect the function of gastric acid gland cells, gastric acid secretion increases, PGI increases, secretion decreases or gastric mucosal glands atrophy, PGI decreases; PGII and gastric fundus The correlation of mucosal lesions is greater (compared to gastric antrum mucosa), and its increase is related to fundic gland duct atrophy, gastric epithelial metaplasia or pseudopyloric gland metaplasia, and dysplasia; the progressive decrease of PGI / II ratio is related to ga...

Examples

Embodiment 1

[0019] A pepsinogen II detection kit, including R1 reagent and R2 reagent, the R1 reagent includes Good's buffer 100mmol / L, sodium chloride 80mmol / L, magnesium chloride 12mmol / L, α-glucosidase 3kU / L, Anti-saliva amylase monoclonal antibody 27mg / L, bovine serum albumin 3g / L, polyvinylpyrrolidone 50mg / L, N-acetylcysteine ​​50mmol / L, and the rest is distilled water; the R2 reagent includes Good's buffer 150mmol / L, 4,6-ethylene-α, D-malto7 glucoside-p-nitrophenol (EPS-G7) 1.6mmol / L, bovine serum albumin 4g / L, ascorbic acid oxidase 1200U / L, and the rest distilled water.

[0020] Reagent R1 reagent and R2 reagent preparation:

[0021] 1) Add bovine serum albumin, concentration of methylisothiazolinone, fatty alcohol and ethylene oxide condensate, magnesium chloride, ammonium formate and sodium phosphate buffer, vortex for 10min, and place at 4-5°C After 2h, centrifuge at 800r / min for 20min, then let it stand for 2 hours, take the supernatant at 3-5°C for later use, and prepare th...

Embodiment 2

[0032] A pepsinogen II detection kit, including R1 reagent and R2 reagent, the R1 reagent includes sodium phosphate buffer 100mmol / L, bovine serum albumin 2g / L, concentration is 3% methylisothiazolinone, fat Alcohol and ethylene oxide condensate 3g / L, magnesium chloride 4mmol / L, ammonium formate 25mmol / L, and the rest is deionized water; the R2 reagent includes pepsinogen II antibody emulsion solution 2mg / ml, bovine serum albumin 2g / L, the concentration is 3% methylisothiazolinone, and the rest is deionized water.

[0033] Reagent R1 reagent and R2 reagent preparation:

[0034] 1) Add bovine serum albumin, concentration of methylisothiazolinone, fatty alcohol and ethylene oxide condensate, magnesium chloride, ammonium formate and sodium phosphate buffer, vortex for 10min, and place at 5°C for 2h Centrifuge at 800r / min for 20min, let stand for 2 hours, take the supernatant at 5°C for later use, and prepare the R1 reagent;

[0035] 2) First, add bovine serum albumin, methylis...

Embodiment 3

[0044] A pepsinogen II detection kit, including R1 reagent and R2 reagent, said R1 reagent includes sodium phosphate buffer 100mmol / L, bovine serum albumin 3g / L, concentration is 2% methylisothiazolinone, fat Alcohol and ethylene oxide condensate 3g / L, magnesium chloride 3mmol / L, ammonium formate 30mmol / L, and the rest is deionized water; the R2 reagent includes pepsinogen II antibody emulsion solution 2mg / ml, bovine serum albumin 2g / L, the concentration is 3% methylisothiazolinone, and the rest is deionized water.

[0045] Reagent R1 reagent and R2 reagent preparation:

[0046] 1) Add bovine serum albumin, concentration of methylisothiazolinone, fatty alcohol and ethylene oxide condensate, magnesium chloride, ammonium formate and sodium phosphate buffer, vortex for 10min, and place at 5°C for 2h Centrifuge at 800r / min for 20min, let stand for 2 hours, take the supernatant at 5°C for later use, and prepare the R1 reagent;

[0047] 2) First, add bovine serum albumin 2-3g / L, ...

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