Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for measuring pepsinogen I/II content of human serum

A technology of pepsinogen and human serum, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unsatisfactory detection of heavy-duty chyle, difficult arrangement of clinical inspection organization, increased workload of operators, etc., and achieve reliable detection results , Strong anti-chyle interference ability, and the effect of reducing the detection time

Inactive Publication Date: 2015-11-18
ZHEJIANG KAICHENG BIOTECH
View PDF8 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in large-scale population census, health check-up screening, and clinical patient testing, a large number of turbid samples such as chyle and hemolysis are often encountered. At this time, by purchasing sample processing agents and processing these samples separately, it will greatly improve the quality of life. Increase the workload of operators, increase operating costs, and also bring difficulties to the organization and arrangement of clinical tests
Chinese patent document CN103698525A discloses a kit that uses lipase or polyethylene glycol-inulin magnesium sulfate to eliminate chyle interference. This method improves the anti-chyle interference ability of the kit, but it is not effective enough for heavy-duty chyle detection ideal; secondly, only methods for pepsinogen I are disclosed, no mention of pepsinogen II

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for measuring pepsinogen I/II content of human serum
  • Kit for measuring pepsinogen I/II content of human serum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A test kit for measuring the content of pepsinogen I and pepsinogen II in human serum, comprising reagents R1, R2 and a calibration solution; the reagent R1 is a buffer solution with a pH value of 7.2-8.6, and the reagent R2 is an anti- Human PGI antibody latex reagent or anti-human PGII antibody latex reagent; the standard product is a recombinant protein containing quantitative PGI or PGII or natural PGI or PGII extracted from human serum; it is characterized in that the kit is also Including reagent R3 for eliminating chyle, said reagent R3 is composed of octyl methoxycinnamate, polyoxypropylene polyoxyethylene propylene glycol ether and polyethylene glycol-magnesium inulin sulfate.

[0039] The parts by weight of the reagent R3 components consist of:

[0040] Octyl Methoxycinnamate 20

[0041] Polyoxypropylene polyoxyethylene propylene glycol ether 20

[0042] Macrogol-Inulin Sulfate Magnesium 100.

[0043] The control measurement was carried out on the serum sam...

Embodiment 2

[0052] The difference with embodiment 1 is:

[0053] The parts by weight of the reagent R3 components consist of:

[0054] Octyl Methoxycinnamate 50

[0055] Polyoxypropylene polyoxyethylene propylene glycol ether 100

[0056] Macrogol-Inulin Sulfate Magnesium 200.

Embodiment 3

[0058] The difference with embodiment 1 is:

[0059] The parts by weight of the reagent R3 components consist of:

[0060] Octyl Methoxycinnamate 30

[0061] Polyoxypropylene Polyoxyethylene Propylene Glycol Ether 40

[0062] Macrogol-Inulin Sulfate Magnesium 120.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a kit for measuring the pepsinogen I / II content of human serum. The kit comprises reagents R1 and R2 and a calibration solution, wherein the reagent R1 is a buffer solution of which the PH value is 7.2 to 8.6, the reagent R2 is an anti-human PGI antibody latex reagent or anti-human PGII antibody latex reagent, and a standard substance is recombinant protein containing quantitative PGI or PGII or natural PGI or PGII extracted from the human serum. The kit is characterized by further comprising a reagent R3, wherein the reagent R3 is used for eliminating chyle and is composed of octyl methoxycinnamate, polyoxypropylene polyoxyethylene propylene glycol ether and polyethylene glycol-sulfate chitosan enzyme. The kit has the advantages of high detection sensitivity and strong anti-chyle interference capability and is suitable for detecting severe chyle.

Description

technical field [0001] The invention relates to a kit for measuring the contents of pepsinogen I and pepsinogen II in human serum by adopting latex-enhanced immune turbidimetry. Background technique [0002] Pepsinogen (PG) is an inactive precursor of pepsin in gastric juice, a precursor of aspartic protease secreted by gastric mucosa, and a single-chain polypeptide with a molecular weight of 42,000 Da, which is transformed into active protease in the stomach. Pepsin. [0003] According to biochemical properties, immunogenicity, cell origin and distribution in tissues, pepsinogen can be divided into two subgroups: pepsinogen Ⅰ (PGⅠ) and pepsinogen Ⅱ (PGⅡ). , called PG I (PGA), mainly secreted by the principal cells of the fundic gland and cervical mucus cells; 6-7 components are called PG II (PGC), in addition to being secreted by the above two cells, the mucus cells of the pyloric gland, Cardiac glands and Brunner glands in the upper part of the duodenum can also produc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573
CPCG01N33/573
Inventor 梁朝阳
Owner ZHEJIANG KAICHENG BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com