Kit for measuring pepsinogen I/II content of human serum

Abstract

The invention relates to a kit for measuring the pepsinogen I/II content of human serum. The kit comprises reagents R1 and R2 and a calibration solution, wherein the reagent R1 is a buffer solution of which the PH value is 7.2 to 8.6, the reagent R2 is an anti-human PGI antibody latex reagent or anti-human PGII antibody latex reagent, and a standard substance is recombinant protein containing quantitative PGI or PGII or natural PGI or PGII extracted from the human serum. The kit is characterized by further comprising a reagent R3, wherein the reagent R3 is used for eliminating chyle and is composed of octyl methoxycinnamate, polyoxypropylene polyoxyethylene propylene glycol ether and polyethylene glycol-sulfate chitosan enzyme. The kit has the advantages of high detection sensitivity and strong anti-chyle interference capability and is suitable for detecting severe chyle.

Description

technical field [0001] The invention relates to a kit for measuring the contents of pepsinogen I and pepsinogen II in human serum by adopting latex-enhanced immune turbidimetry. Background technique [0002] Pepsinogen (PG) is an inactive precursor of pepsin in gastric juice, a precursor of aspartic protease secreted by gastric mucosa, and a single-chain polypeptide with a molecular weight of 42,000 Da, which is transformed into active protease in the stomach. Pepsin. [0003] According to biochemical properties, immunogenicity, cell origin and distribution in tissues, pepsinogen can be divided into two subgroups: pepsinogen Ⅰ (PGⅠ) and pepsinogen Ⅱ (PGⅡ). , called PG I (PGA), mainly secreted by the principal cells of the fundic gland and cervical mucus cells; 6-7 components are called PG II (PGC), in addition to being secreted by the above two cells, the mucus cells of the pyloric gland, Cardiac glands and Brunner glands in the upper part of the duodenum can also produc...

AI Technical Summary

Problems solved by technology

However, in large-scale population census, health check-up screening, and clinical patient testing, a large number of turbid samples such as chyle and hemolysis are often encountered. At this time, by purchasing sample processing agents and processing these samples separately, it will greatly improve the quality of life. Increase the workload of operators, increase operating costs, and also bring difficulties to the organization and arrangement of clinical tests

Chinese patent document CN103698525A discloses a kit that uses lipase or polyethylene glycol-inulin magnesium sulfate to eliminate chyle interference. This method improves the anti-chyle interference ability of the kit, but it is not effective enough for heavy-duty chyle detection ideal; secondly, only methods for pepsinogen I are disclosed, no mention of pepsinogen II

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