The invention relates to the technical field of beverage production. In order to solve the problems of single flavor and poor stability in shelf life of traditional milk tea beverages, the invention provides a hot-filled PET bottle milk tea beverage containing Japanese Okinawa black sugar and a preparation method of the milk tea beverage. Every 1000mL of the hot-filled PET bottle milk tea beveragecomprises the following components: 30-80g of whole milk powder, 2-30g of skimmed milk powder, 5-100g of white granulated sugar, 2-15g of coconut oil, 2-20g of black tea, 0.1-5g of Japanese Okinawa black sugar, 0.1-3g of an emulsifier, 0.1-20g of a thickening agent, 0.01-0.05g of a phosphate, 0.01-1g of an antioxidant, 0.05-0.5g of nisin, 0.1-1.5g of an acidity regulator and the balance of purified water. The formula disclosed by the invention is based on milk, tea, black sugar and coconut oil; specific components and content proportions are adopted for synergistic proportioning, so that compared with common milk tea, the hot-filled PET bottle milk tea beverage is smoother in taste, is healthier and is more stable in shelf life. the preparation method is simple to operate, has no specialrequirement on equipment and is easy for industrialization.