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A three-layer embedding method for improving the stability of lactic acid bacteria

A three-layer embedding, lactic acid bacteria technology, applied in the direction of fixation on/in organic carriers, bacteria, preservation microorganisms, etc., can solve problems such as poor stability, achieve high fluidity, prolong shelf life activity, low hygroscopicity Effect

Active Publication Date: 2022-05-03
河北一然生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The invention proposes a three-layer embedding method for improving the stability of lactic acid bacteria, which solves the problem of poor stability in the prior art

Method used

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  • A three-layer embedding method for improving the stability of lactic acid bacteria
  • A three-layer embedding method for improving the stability of lactic acid bacteria
  • A three-layer embedding method for improving the stability of lactic acid bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0031] S1, carry out high-density culture to bacterial strain in fermentation tank according to existing technology, make the number of live bacteria of Lactobacillus plantarum in fermented liquid reach 8.5×10 9 cfu / ml, use a butterfly centrifuge to centrifuge the fermentation liquid under the condition that the bacteria are not damaged, and concentrate the fermentation liquid by 70 times to obtain the bacteria sludge;

[0032] S2. Add embedding protection agent 1 to the bacteria slime according to the mass ratio of 1:3, wherein the embedding protection agent 1 is 5% skimmed milk powder, 95% water (mass ratio), and use a homogenizer to mix the above bacteria slime and The emulsion after the protective agent is mixed is fully mixed;

[0033] S3. Add the embedding protectant 2 according to the mass ratio of bacteria slime: protectant 2 of 1:3, wherein the embedding protectant 2 is 2% trehalose, 0.5% mannitol, 0.5% water-soluble vitamin E, 0.5% Sodium glutamate, 1% β-cyclodextri...

Embodiment 2

[0037] S1, carry out high-density culture to bacterial strain in fermentation tank according to existing technology, make the number of live bacteria of Lactobacillus plantarum in fermented liquid reach 8.5×10 9 cfu / ml, use a butterfly centrifuge to centrifuge the fermentation liquid under the condition that the bacteria are not damaged, and concentrate the fermentation liquid by 70 times to obtain the bacteria sludge;

[0038] S2. Add embedding protectant 1 to the bacteria slime according to the mass ratio of 1:2, wherein the embedding protector 1 is 30% skimmed milk powder and 70% water (mass ratio), and use a homogenizer to mix the above bacteria slime and The emulsion after the protective agent is mixed is fully mixed;

[0039] S3. Add the embedding protectant 2 according to the mass ratio of bacteria slime: protectant 2 of 1:2, wherein the embedding protectant 2 is 10% trehalose, 3% mannitol, 3% water-soluble vitamin E, 3% Sodium glutamate, 6% β-cyclodextrin, 6% resistan...

Embodiment 3

[0043] S1, carry out high-density culture to bacterial strain in fermentation tank according to existing technology, make the number of live bacteria of Lactobacillus plantarum in fermented liquid reach 8.5×10 9 cfu / ml, use a butterfly centrifuge to centrifuge the fermentation liquid under the condition that the bacteria are not damaged, and concentrate the fermentation liquid by 70 times to obtain the bacteria sludge;

[0044] S2. Add embedding protectant 1 to the bacteria slime according to the mass ratio of 1:1, wherein the embedding protector 1 is 50% skimmed milk powder and 50% water (mass ratio), and use a homogenizer to mix the above bacteria slime and The emulsion after the protective agent is mixed is fully mixed;

[0045] S3. Add the embedding protectant 2 according to the mass ratio of bacteria slime: protectant 2 of 1:1, wherein the embedding protectant 2 is 20% trehalose, 5% mannitol, 5% water-soluble vitamin E, 5% Sodium glutamate, 10% β-cyclodextrin, 10% resist...

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Abstract

The present invention relates to the technical field of microbial preparations, and proposes a three-layer embedding method for improving the stability of lactic acid bacteria, including S1, carrying out high-density culture of lactic acid bacteria in a fermenter; S2, centrifuging the bacteria sludge from the fermentation liquid, The moisture content of the bacteria slime is 5% to 50%; S3. Mix the bacteria slime and the first layer of embedding protection agent according to the mass ratio of 1: (1 to 3), and the first layer of embedding agent contains 5% to 50% The first layer of embedding protectant of skim milk powder; S4, mix the bacteria slime and the second layer of embedding protector according to the mass ratio of 1: (1~3) and mix them with S3 evenly; S5, mix the bacteria slime and the third layer of embedding protector Layer embedding protection agent according to the mass ratio of 1: (1~3) mixed with the mixture obtained in S4 and then emulsified evenly to obtain S5 emulsion; The body enters a dormant state instantly to obtain S6 cryogenic granules; S7, drying the S6 cryogenic granules until the water content is 3% to 8%. Through the above technical solution, the problem of poor stability in the prior art is solved.

Description

technical field [0001] The invention relates to the field of microbial preparations, in particular to a three-layer embedding method for improving the stability of lactic acid bacteria. Background technique [0002] As early as the 1970s, researchers in developed countries such as Europe, America, Japan and South Korea began to develop new microecological agents to partially or completely replace the use of antibiotics and hope to improve human immunity. After decades of development, it has been Some achievements have been made in related fields. After the reform and opening up, with the development of my country's economy, people's understanding of the health system has continued to deepen, and they have gradually realized that lactic acid bacteria microecological preparations have great advantages in regulating the balance of intestinal flora and improving immunity. [0003] However, due to the poor stress resistance of lactic acid bacteria, even if the bacterium is place...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/10C12N11/04C12N1/20C12N1/04
CPCC12N11/10C12N11/04C12N1/20C12N1/04
Inventor 赵林森申朋杨玲郭红敏马新颖任磊路江浩张士成刘佳霍世和
Owner 河北一然生物科技股份有限公司
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