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Latex enhanced immunoturbidimetry assay kit for pepsinogen I/II

An immunoturbidimetric and latex-enhanced technology, applied in the biological field, achieves the effect of simple operation, good stability, and no significant difference

Inactive Publication Date: 2014-10-22
唐勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Gastric cancer screening began in Japan in the 1960s, when gastric cancer was highly common. At that time, a step-by-step screening method of gastrointestinal double-contrast radiography-gastroscopy-pathological examination was adopted, which cost a lot of manpower and material resources.

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  • Latex enhanced immunoturbidimetry assay kit for pepsinogen I/II
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  • Latex enhanced immunoturbidimetry assay kit for pepsinogen I/II

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Embodiment Construction

[0033] The invention discloses a kit for detecting the content of PGI / PGII. Those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications that are obvious to those skilled in the art are deemed to be included in the present invention. The products and applications of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the methods and applications described herein without departing from the content, spirit and scope of the present invention. The technology of the present invention.

[0034] The present invention will be further described below with specific examples.

[0035] 1. Preparation of PGI / PGII reagent R1, i.e. sample buffer

[0036] Reagent Dosage Sodium dihydrogen phosphate 2....

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Abstract

The invention relates to a kit for measurement of the contents of pepsinogen I (PGI) and pepsinogen II (PGII) in serum. To overcome a technical problem, the invention provides the PGI and PGII content measurement kit applicable to a nephelometric instrument or fully automatic biochemical analyzer. The kit comprises the following components: a, R1, which is composed of a buffer solution, an accelerator and a surfactant, with the balance being purified water; b, R2-I, which is composed of a buffer solution and a latex microsphere binding with an antibody against human PGI; c, R2-II, which is composed of a buffer solution and a latex microsphere binding with an antibody against human PGII; d, a calibrator 1, which is composed of a buffer solution, a stabilizing agent, an antiseptic and a certain amount of pure recombinant PGI protein, with the balance being purified water; and e, a calibrator 2, which is composed of a buffer solution, a stabilizing agent, an antiseptic and a certain amount of pure recombinant PGII protein, with the balance being purified water Through combination of the above-mentioned reagents, the kit can rapidly measure the contents of PGI and PGII in serum.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit for measuring the contents of pepsinogen I and pepsinogen II in human serum by using a latex-enhanced immunoturbidimetric method. Background technique [0002] Pepsinogen (PG) is a precursor of aspartic acid protease, a single-chain polypeptide with a molecular mass of 42kDa, including three disulfide bonds, and an isoelectric point of 3.7. Pepsin is divided into two subgroups according to its protochemical properties and immunogenicity. Components 1-5 have the same immunogenicity and are called pepsinogen I (PGI), which is mainly composed of the principal cells of the fundic gland and the mucus neck. Secreted by cells; components 6 and 7 are called pepsinogen II (PGII) and are secreted by the mucous neck cells of the oxyntic glands, cardia glands, and antrum of the gastric body and fundic mucosa in addition to PGII is also produced by the mucous cells of the pyloric glands o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/531
CPCG01N33/573G01N33/54393G01N2800/06
Inventor 唐勇
Owner 唐勇
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