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United quantitative detection test paper of gastric cancer risk markers and preparation method

A technology for quantitative detection and detection test paper, which is applied in the field of biomedicine and can solve the problems of high detection sensitivity, low sensitivity and complicated operation process.

Inactive Publication Date: 2018-12-14
广州华澳生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Latex-enhanced immunoturbidimetric assay, enzyme-linked immunoassay, time-resolved fluorescent immunoassay, the operation process is complicated, requires a lot of instruments and equipment and professional operation, the detection time is long, and the detection sensitivity is high
Colloidal gold immunochromatography is simple to operate, but the sensitivity is not high, and it cannot be accurately quantified

Method used

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  • United quantitative detection test paper of gastric cancer risk markers and preparation method
  • United quantitative detection test paper of gastric cancer risk markers and preparation method
  • United quantitative detection test paper of gastric cancer risk markers and preparation method

Examples

Experimental program
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Embodiment

[0036] Example, the establishment of a combined quantitative detection method for gastric cancer risk markers:

[0037] 1.1 Preparation of polystyrene microsphere seeds

[0038] 1.1.1 Measure 100ml of styrene and put it in a separatory funnel, wash it repeatedly with 1 M NaOH 3 times to remove the impurities in styrene, then wash it with purified water until neutral, then put it in a drying dish to dry for more than 24 hours;

[0039] 1.1.2 Fill the reaction device with argon gas to remove the oxygen in the device;

[0040] 1.1.3 Add 100ml of ethanol to the reaction device, then add 5g of PVP, and stir for 30min;

[0041] 1.1.4 Add 40ml of washed styrene and 5ml of azobisisobutyronitrile (AIBN), raise the temperature of the water bath to 45°C, react for 30min, then raise the reaction temperature to 75°C, and react for 12h;

[0042] 1.1.5 After the reaction is over, filter the above reaction solution with a mesh sieve, then wash and centrifuge with absolute ethanol for 3 to 5...

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Abstract

The invention relates to united quantitative detection test paper of gastric cancer risk markers and a preparation method. The gastric cancer risk markers include pepsinogen I, pepsinogen II and gastrin 17. A test paper strip comprises a sample pad, a gastric cancer risk marker antibody magnetic rare earth fluorescent microsphere labelling pad, a coating membrane and a water absorption pad, wherein three gastric cancer risk marker quantitative detection lines and a quality control region C line are arranged on the coating membrane. After tested substances in samples are concentrated through amagnetic field, a low-background high-sensitivity fluorescence immunochromatographic method is utilized for detecting the tested substances, and the problem of low sensitivity of a POCT type product is solved. The problem that when blood samples are detected through an immunity lateral chromatography reagent, in order to reduce the interference of other substances, usually the samples are tested after being diluted, so that the sensitivity is slightly low can be solved.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a gastric cancer risk marker joint quantitative detection test paper and a preparation method thereof based on magnetic rare earth fluorescent microspheres as tracer markers, specifically, a method for measuring blood (serum, plasma, whole Reagent card for detecting the content of pepsinogen Ⅰ / pepsinogen Ⅱ / gastrin 17 in blood). Background technique [0002] Pepsinogen (PG), the precursor of pepsin, is divided into two subgroups based on their biochemical properties and immunogenicity. The same immunogenicity of components 1-5 is called PG I, which is mainly secreted by the principal cells and mucus neck cells of the fundic gland, and components 6-7 are called PG II, except for the secretion by the chief cells and mucus neck cells of the fundic gland. In addition to cell secretion, pepsinogen II can also be produced by the mucous neck cells of the cardia glands and pyloric glands of the ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574G01N33/533
CPCG01N33/533G01N33/57446G01N33/6893
Inventor 于龙波于永涛黎权刘日俊
Owner 广州华澳生物科技有限公司
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