Pepsinogen I/II determination kit and preparation method thereof

Abstract

The invention provides a pepsinogen I / II determination kit and a preparation method thereof. A magnetic bead diluent in the kit comprises 0.04-0.06 mol / L of HEPES, 9-11 g / L of protein, 1-4 g / L of a protective agent, 29-31 g / L of sucrose, 1.5-2.5 g / L of S17 and 0.5-1.5 g / L of a preservative, and the pH value is 7.07.5. The enzyme-labeled diluent comprises 0.04 to 0.06 mol / L of MES, 19 to 21 g / L ofcasein, 39 to 41 g / L of sucrose, 1 to 5 g / L of a dispersant, 0.05 to 0.15 g / L of a blocking agent, 0.13 to 0.14 g / L of ZnCl2, 1 to 1.5 g / L of MgCl2, 1.5 to 2.5 g / L of Tween 20, 0.5 to 1.5 g / L of a preservative and 8 to 9 g / L of NaCl, the PH value is 6.0-6.5; and the stability is high.

Description

technical field

[0001] The invention belongs to the technical field of immunoassay, and relates to a pepsinogen I / II assay kit and a preparation method thereof. Background technique

[0002] Pepsinogen is the inactive precursor of pepsin. It is divided into 2 subgroups according to its biochemical properties and immunogenicity. Components 1-5 have the same immunogenicity and are called pepsinogen I. It is mainly composed of gastric fundus Secreted by chief cells and mucous neck cells of the gland; components 6 and 7 are called pepsinogen II, in addition to being secreted by chief cells and mucous neck cells of the fundic glands, mucous neck cells of the pyloric glands of the cardia glands and antrum And the upper duodenum can also produce pepsinogen II. Most of the PG synthesized in the stomach enters the gastric cavity and is activated into pepsin under the action of acidic gastric juice. Only a small amount of PG (about 1%) enters the blood circulation through the capilla...

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