This invention relates to the field of
food safety testing technology and discloses a method for determining the residual amounts of
amide alcohols and their metabolites in milk. The method involves adding an
internal standard to a
milk sample, followed by the addition of a composite
desorption solvation reagent and shaking to induce the
desorption of the target
analyte from its
protein-
bound state into the free aqueous phase. Subsequently, magnetic HLB microparticles are introduced and an external
magnetic field is applied to anchor the microparticles to the inner wall of the container for
spatial isolation. Under these conditions, low-speed horizontal cross-flow is applied, and a
phase transition triggering and cross-flow
flocculation reagent is added dropwise to induce
flocculation of
protein impurities at the center of the
system, simultaneously driving the free target
analyte phase to transfer to the internal pores of the inner wall microparticles. Finally, after draining the central
flocculation waste liquid, matrix washing, and targeted
elution, quantitative analysis is performed using liquid
chromatography-
tandem mass spectrometry. This invention, through the combination of in-situ
desorption and cross-flow phase transfer, avoids
protein flocculants clogging the extraction channels, achieving simultaneous and efficient enrichment of impurities and target analytes.