The invention discloses giant salamander grease compound burn medicine and a preparation method of the giant salamander grease compound burn medicine. The giant salamander grease compound burn medicine comprises, by weight, coptis chinensis 1g, scutellaria baicalensis 1g, frankincense 0.5g, radix angelicae 0.5g, bletilla striata 0.5g, sesame oil 15.624g-40g, beewax 1.8172g, borneol 0.1g and giant salamander oil 4g-12g. The giant salamander grease compound burn medicine has the fragrance of the sesame oil. According to the giant salamander grease compound burn medicine, the special greasy sense can create moist environment and is beneficial to the release of multiple growth factors; migration rate and scabbing of epidermic cells are quickened; due to the dryness, wound epidermis tension force is strengthened, and therefore cicatrix growth is avoided and wound healing is quickened; an anoxic state created by the giant salamander grease compound burn medicine can restrain the growth of bacteria. The stability of the plaster is explained by the fact that unusual conditions such as delamination in a centrifuge model test and a heat - resistant and cold- resistant test do not occur. In an acute toxicity test, tissues such as the heart, the liver, the spleen, the lungs, the kidney, the brain, the intestine, the aorta, the thymus, the testis and the epididymis are not subject to pathologic change, which explains that the giant salamander grease compound burn medicine has no acute toxicity.