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Cultivating method for high-oleic acid rapeseed variety

A breeding method and high oleic acid technology, applied in the field of breeding excellent high oleic rapeseed strains and cultivating high oleic rapeseed varieties, can solve the problems of no high oleic rapeseed varieties, reduce workload and improve accuracy Effect

Inactive Publication Date: 2016-06-01
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no high oleic rape variety approved in China

Method used

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  • Cultivating method for high-oleic acid rapeseed variety
  • Cultivating method for high-oleic acid rapeseed variety
  • Cultivating method for high-oleic acid rapeseed variety

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Embodiment 1

[0020] (1) Prepare the F1 combination with normal oleic acid content and high oleic acid content

[0021] Brassica napus line A A177 is used as the female parent (oleic acid content 65%) [the seeds of this material have been sent to China. Wuhan. Wuhan University China Type Culture Collection Center for preservation on November 20, 2009, and its preservation number is CCTCCNO: P200908; the biological material has been in the patent No. ZL2009102734352, and the application publication number is CN101824472A. In this application, Brassica napus strain A A177 has been disclosed in the applicant’s patent literature. This application does not need to resubmit the biological material preservation and Open), and the created high oleic acid content Brassica napus A H003 as the male parent (the oleic acid content measured in 2012 was 80%. The applicant named the male parent as rape high oleic acid seed A H003, Brassicanapus L. A H003 was sent to China. Wuhan. Wuhan University China Typ...

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Abstract

The invention belongs to the field of rapeseed breeding, and particularly relates to a cultivating method for a high-oleic acid rapeseed variety. The cultivating method is characterized by comprising the following steps: performing hybridization by taking first Brassica napus L. A177 with the oleic acid content of 65 percent or below as a female parent and taking first high-oleic acid Brassica napus L. H003 which has the preservation number of CCTCC NO:P201604 and has the oleic acid content of at least 78 percent as a male parent, and obtaining F1 through artificial emasculation; planting the F1, obtaining a separated DH line through microspores culture; performing high-oleic acid molecular marker identification on strain seedling stage of a DH line group, and determining the genotype of each strain; measuring the content of oleic acid of each single plant by utilizing a gas chromatograph, and determining the content of the oleic acid of the each primarily selected line; selecting the parent strains of which the characters, such as the whole plant pod number, the seed number per pod, the thousand seed weight and the yield per plant, are superior to the parent strain with the oleic acid content lower than 69 percent from the DH strain with the oleic acid content of greater than 75 percent to obtain the high-oleic acid rapeseed selected line and variety of which the oleic acid is greater than 78 percent.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to a method for cultivating high-oleic-acid rapeseed varieties. The invention relates to a method for breeding excellent high-oleic rapeseed strains by combining microspore culture, molecular marker-assisted selection and gas chromatography. Background technique [0002] The composition of fatty acids in rapeseed directly determines the edible quality and nutritional value of rapeseed oil. Oleic acid is an octadecyl monounsaturated fatty acid. The oleic acid content in ordinary rape seeds is 17-65%. The high oleic rapeseed in the present invention refers to the rapeseed varieties and the derived rapeseed oils whose oleic acid ratio in seeds and rapeseed oil fatty acids is greater than 75%. [0003] High oleic edible oils, including high oleic rapeseed oil, have high application value in edible oil and industry. High oleic acid edible oil has high oxidation s...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04C12N3/00C12Q1/68
CPCA01H1/02A01H1/04C12N3/00C12Q1/6895C12Q2600/13
Inventor 周永明蔡光勤赵青杨庆勇范楚川张椿雨
Owner HUAZHONG AGRI UNIV
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