Breeding method of rape with high-density siliques on main inflorescence and application
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A main inflorescence, high-density technology, applied in the fields of application, botanical equipment and methods, plant genetic improvement, etc., to achieve the effect of improving oil menu production
Active Publication Date: 2016-07-20
贵州省油菜研究所
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[0005] The object of the present invention is: to provide a kind of breeding method and application of rapeseed with high density of main inflorescence and high density of main inflorescence of rapeseed, w
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Embodiment 1
[0018] Embodiment 1 of the present invention: Breeding of Rapeseed Rapeseed with High Density of Main Inflorescence:
[0019] 1) Hybridization test: The Brassica napus variety Zhongshuang 2 was used as the female parent and the rapeseed variety Jingyou 2 was used as the male parent to obtain F 1 , bagging self-breeding to obtain segregated populations;
[0020] 2) Obtaining high-density silique rape in the main inflorescence: to F 2 The traits of plant height, number of primary branches, main inflorescence length, number of main inflorescence siliques, main inflorescence silique density, length of main inflorescence siliques, and number of seeds per horn were identified, and one plant with main inflorescence silique density>2.0 / cm of rapeseed strains. After multi-point (Guiyang Jinyang, Guizhou Kaiyang) and multi-year (2007-2011) identification, the plant type, plant height, branch and main inflorescence are very stable. These traits are specifically manifested as: t...
Embodiment 2
[0022] Embodiment 2 of the present invention: Genetic analysis and identification of traits of high-density siliques in main inflorescence:
[0023] F 1 (P 1 ×P 2 ), F 1 F 2 , F 2F 2:3 Population, using the main gene + polygene mixed genetic model analysis method for genetic analysis. The maximum likelihood method and IECM (Iterated Expectation and Conditional Maximization) algorithm are used to estimate the parameters of each generation and each component distribution, and the best model is selected through the AIC (Akaike Information Criterion) value, and a set of suitability tests, including uniformity U 1 2 , U 2 2 with U 3 2 test, Smimov test ( n W 2 ) and the Kolmogorov test ( D n) , choose the optimal genetic model according to the test results. Finally, the least squares method was used to estimate the genetic parameters such as the effect value and variance of each gene based on the distribution parameters of each component of the optimal model. ...
Embodiment 3
[0024] Embodiment 3 of the present invention: Application of using high-density silique rapeseed in main inflorescence to breed high-density silique rapeseed in main inflorescence:
[0025] F 1 , a segregated population was obtained by selfing, with a total of 674 F 2 For a single plant, high-density silique materials with a main inflorescence density of about 2.3 siliques / cm were obtained through continuous self-crossing and backcrossing.
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Abstract
The invention discloses a breeding method of rape with high-density siliques on a main inflorescence and application. The breeding method comprises the following steps: adopting double-2 in the variety of cabbage type rape and nepeta oil-2 in the variety of cabbage type rape as parents for hybridization to obtain F1, and conducting bagged selfing propagation to obtain a segregated population; selecting single plants in the segregated population to conduct identification on phenotypic characteristics in the field continuously for 4 years, selecting single plants with high-density siliques on the main inflorescence (number of effective siliques on the main influorescence/main-inflorescence length being greater than 2 pieces/cm); in a flowering period, selecting flower buds 2.5-3.5mm in length on the main inflorescence, using colchicine to double chromosomes, using microspores for culture to obtain a double-haploid population, and selecting single plants to breed into strain with high-density siliques on the main inflorescence and stable characters; using the strain and double 11 in the variety of cabbage type rape (the density of siliques on the main inflorescence is about 1.7 pieces/cm) to conduct hybridization, utilizing F2:3 populations to conduct genetic segregation analysis and finding that the characters of the high-density siliques are controlled by 2 pairs of additive-dominant-epistatic main genes. The breeding method and the application disclosed by the invention have the advantages that the variety of rape with high-density siliques on the main inflorescence can be bred by utilizing hybridization of the high-density silique materials on the main inflorscence and common rape, and the yield per unit of the rape can be greatly increased.
Description
technical field [0001] The invention relates to the technical field of rapeseed breeding, in particular to a breeding method and application of high-density silique rapeseed in the main inflorescence. Background technique [0002] Brassica napus belongs to the Brassicaceae ( Cruciferae ) Brassica ( brassica ), is the second largest bulk oil crop in the world, not only the main source of edible oil, but also an important economic crop that provides biodiesel and other industrial raw materials. China's annual planting area exceeds 7 million hectares (100 million mu), and the rapeseed output exceeds 10 million tons, ranking first in the world in both area and total output. In recent years, the gap between domestic supply and demand has been increasing. In 2014, under the condition of controlling the import volume, the import volume of rapeseed was as high as 5.081 million tons, and the rapeseed oil was 810,000 tons. It has become the world's major rapeseed importer. As the ...
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