Breeding method of rape with high-density siliques on main inflorescence and application
A main inflorescence, high-density technology, applied in the fields of application, botanical equipment and methods, plant genetic improvement, etc., to achieve the effect of improving oil menu production
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Embodiment 1
[0018] Embodiment 1 of the present invention: Breeding of Rapeseed Rapeseed with High Density of Main Inflorescence:
[0019] 1) Hybridization test: The Brassica napus variety Zhongshuang 2 was used as the female parent and the rapeseed variety Jingyou 2 was used as the male parent to obtain F 1 , bagging self-breeding to obtain segregated populations;
[0020] 2) Obtaining high-density silique rape in the main inflorescence: to F 2 The traits of plant height, number of primary branches, main inflorescence length, number of main inflorescence siliques, main inflorescence silique density, length of main inflorescence siliques, and number of seeds per horn were identified, and one plant with main inflorescence silique density>2.0 / cm of rapeseed strains. After multi-point (Guiyang Jinyang, Guizhou Kaiyang) and multi-year (2007-2011) identification, the plant type, plant height, branch and main inflorescence are very stable. These traits are specifically manifested as: t...
Embodiment 2
[0022] Embodiment 2 of the present invention: Genetic analysis and identification of traits of high-density siliques in main inflorescence:
[0023] F 1 (P 1 ×P 2 ), F 1 F 2 , F 2F 2:3 Population, using the main gene + polygene mixed genetic model analysis method for genetic analysis. The maximum likelihood method and IECM (Iterated Expectation and Conditional Maximization) algorithm are used to estimate the parameters of each generation and each component distribution, and the best model is selected through the AIC (Akaike Information Criterion) value, and a set of suitability tests, including uniformity U 1 2 , U 2 2 with U 3 2 test, Smimov test ( n W 2 ) and the Kolmogorov test ( D n) , choose the optimal genetic model according to the test results. Finally, the least squares method was used to estimate the genetic parameters such as the effect value and variance of each gene based on the distribution parameters of each component of the optimal model. ...
Embodiment 3
[0024] Embodiment 3 of the present invention: Application of using high-density silique rapeseed in main inflorescence to breed high-density silique rapeseed in main inflorescence:
[0025] F 1 , a segregated population was obtained by selfing, with a total of 674 F 2 For a single plant, high-density silique materials with a main inflorescence density of about 2.3 siliques / cm were obtained through continuous self-crossing and backcrossing.
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