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Simple method for artificially synthesizing cabbage type rape

A technology of Brassica napus and a simple method, which is applied in the field of artificial synthesis of Brassica napus, can solve the problems of hybridization incompatibility between cabbage and Chinese cabbage species, such as workload, low efficiency, and long time consumption, so as to overcome incompatibility and The effect of heavy workload, high efficiency and small workload

Active Publication Date: 2013-07-10
HUNAN AGRICULTURAL UNIV +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: aiming at the incompatibility and heavy workload, time-consuming, complicated and low-efficiency problems of interspecies hybridization between cabbage and Chinese cabbage in the prior art, to provide a simple operation, small workload, A Simple and Efficient Method for Artificially Synthesizing Brassica napus

Method used

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  • Simple method for artificially synthesizing cabbage type rape
  • Simple method for artificially synthesizing cabbage type rape
  • Simple method for artificially synthesizing cabbage type rape

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Example 1: Brassica napus artificially synthesized by cabbage G3 strain and Chinese cabbage B1 strain

[0016] 1. Material planting: On October 10, 2010, the cabbage G3 strain and the Chinese cabbage B1 strain were planted in the rape test field. On March 10, 2011, the cabbage B1 entered the flowering period, and on March 23, the cabbage G3 entered the flowering period.

[0017] 2. Hybridization: The early flowering cabbage B1 is used as the male parent, and the relatively late flowering cabbage G3 is used as the female parent. Between 9 and 10 in the morning, on the inflorescence of cabbage G3, select 11 flower buds whose petals are about to unfold but have not yet opened, and remove all other flower buds that have opened and have not opened; then completely remove the stamens of the 11 flowers, and remove the The pollen of B1 was pollinated on the stigma of cabbage G3, and bagged with a sulfuric acid paper bag to prevent pollen from other sources from falling on the s...

Embodiment 2

[0020] Example 2: Artificially synthesizing Brassica napus using Brassica oleracea G2 strain and Chinese cabbage B2-2 strain

[0021] Planting of materials: On October 10, 2011, the cabbage G2 strain and the Chinese cabbage B2-2 strain were planted in the rape test field. On March 10, 2012, the Chinese cabbage B2-2 entered the flowering period, and on March 23, the G2 entered the flowering period.

[0022] Hybridization: The early flowering cabbage B2-2 was used as the male parent, and the relatively late flowering cabbage G2 was used as the female parent. Between 9 and 10 in the morning, on the inflorescence of cabbage G2, select 18 flower buds whose petals are about to unfold but have not yet opened, and remove all other flower buds that have opened and have not opened; then completely remove the stamens of the 18 flowers, and remove the The pollen of B2-2 was pollinated on the stigma of cabbage G2, and bagged with a sulfuric acid paper bag to prevent pollen from other sourc...

Embodiment 3

[0025] Example 3: Brassica napus artificially synthesized by cabbage G4 strain and Chinese cabbage B1 strain

[0026] Material planting: On October 10, 2011, the cabbage G4 strain and the Chinese cabbage B1 strain were planted in the rape test field. On March 10, 2012, the Chinese cabbage B1 entered the flowering period, and on March 23, the G4 entered the flowering period.

[0027] Hybridization: The early flowering cabbage B1 was used as the male parent, and the relatively late flowering cabbage G4 was used as the female parent. Between 9 and 10 in the morning, on the inflorescence of cabbage G4, select 23 flower buds whose petals are about to unfold but have not yet opened, and remove all other flower buds that have opened and have not opened; then completely remove the stamens of the 23 flowers, and remove the The pollen of B1 was pollinated on the stigma of cabbage G4, and bagged with a sulfuric acid paper bag to prevent pollen from other sources from falling on the stigm...

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Abstract

The invention discloses a simple method for artificially synthesizing a cabbage type rape. The simple method comprises the following steps of: when wild cabbage and Chinese cabbage are hybridized, carrying out fertilization on the stigma of a female parent; after 16-144 hours, directly spraying a colchicine aqueous solution with percent concentration of 0.05%-0.15% on young ovary; and after the ovary grows to naturally-matured silique, harvesting full seeds, i.e., obtaining artificially-synthesized wild cabbage type rape seeds. Therefore, a new means with convenience, fastness and high efficiency is provided for obtaining the new germplasm of the cabbage type rape.

Description

technical field [0001] The invention relates to the field of crop genetic breeding, in particular to a simple and convenient method for artificially synthesizing Brassica napus. Background technique [0002] Artificial synthesis of Brassica napus is an effective way to broaden the germplasm resources of Brassica napus genetics and breeding, and it is also one of the important means to study the origin and evolution of Brassica species. In the process of synthesizing Brassica napus, due to the incompatibility and the number of chromosomes in interspecific hybridization between Brassica oleracea (CC=20) and Chinese cabbage (AA=18), it is difficult for heterozygous or heterozygous embryos to develop into normal embryos. ; even if the hybrid F1 (AC=19) seeds were obtained, they were infertile and required chromosome doubling to obtain fertile allotetraploid Brassica napus (AACC=38). At present, in order to obtain artificially synthesized Brassica napus, the following methods ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/08
Inventor 刘春林阮颖何文
Owner HUNAN AGRICULTURAL UNIV
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