High-temperature tolerance high-efficiency screening and identifying method in filling stage of rapeseeds

Abstract

The invention discloses a high-temperature tolerance high-efficiency screening and identifying method in filling stage of rapeseeds. The high-temperature tolerance high-efficiency screening and identifying method comprises the following steps of selecting the rape materials; planting the to-be-screened rape materials under the conventional condition; marking the flowers which flower at the same day, and obtaining a plurality of even number of siliques; mixing the siliques of each type of rape material, averagely separating into two groups, respectively inserting all siliques into a plant culture medium via silique stems, and respectively putting containers loaded with the siliques of each type of rape material into a plant growth box or a plant growth chamber for proper-temperature groupsand high-temperature groups; setting the culture environment of the siliques; measuring the related biology indexes of the high-temperature tolerance in the filling stage of each treated sample; screening the high temperature-resistant rape material. The high-temperature tolerance high-efficiency screening and identifying method has the advantages that the operability is strong, the accuracy is high, the repeatability is good, the identifying efficiency is high, the time is shortened, and the energy consumption is decreased; the wide application prospect is realized in the breeding of heat-resistant varieties, and predicting of ecological suitability of varieties, and the technical foundation is provided for the genetic improvement of the high-temperature tolerance in the filling stage ofthe rapeseeds.

Description

technical field [0001] The invention relates to the technical field of crop breeding, in particular to a high-efficiency screening and identification method for high temperature tolerance of rapeseed seeds at the filling stage. Background technique [0002] High temperature stress seriously affects the growth and development of crops, and is the main threat to the stable and high yield of crops. The average daily temperature during the seed development period of rapeseed is negatively correlated with the oil content. During the maturity period of rapeseed, the temperature is above 20°C, sufficient light, large temperature difference between day and night, and suitable soil humidity are conducive to oil accumulation, high yield and oil content; while the temperature is too high, it will lead to "High temperature forces ripening", the aging of plants is accelerated, the filling period is shortened, and the thousand-grain weight and oil content are reduced. In the past hundred...

AI Technical Summary

Problems solved by technology

This method has the characteristics of objective evaluation, but the test results are easily affected by other environmental factors, and the repeatability is poor. In order to obtain reliable results, repeated identification of multiple points for many years must be carried out, which is labor-intensive and time-consuming.

The artificial simulation direct identification method is to evaluate the high temperature tolerance of crops through visual trait change indicators under simulated high temperature stress conditions. This method can overcome the shortcomings of field identification, and the adverse conditions are easy to control, but it is limited by equipment investment and energy consumption. Due to the limitation of various factors, it is difficult to identify large quantities of materials, especially not suitable for large-scale screening and identification of tall crops such as rapeseed

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