Hemicellulase, and coding gene and application thereof

Abstract

The invention discloses hemicellulase, and a coding gene and pplication thereof. The amino acid residue sequence of the hemicellulase is shown as (a) or (b): (a) an amino acid residue sequence shown as SEQ ID No:1; (b) an amino acid residue sequence which has the regulation and control effects on plant silique cracking and is obtained through the substitution and / or deletion and / or addition of one or several amino acid residues on the amino acid residue sequence shown as SEQ ID No:1. After the hemicellulase gene is mutated or subjected to suppression expression, the silique cracking time can be obviously delayed; meanwhile, the plant silique cracking rate is reduced, so that the seed yield reduction and the land pollution loss caused by relevant crop silique early cracking due to environment factors are reduced; further, the high yield of crop seeds is ensured; high practical application value is realized; the application prospects are wide.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to a hemicellulase, its encoding gene and its application. Background technique [0002] In the life process of plants, the separation of cells plays a very important role: from seed germination, to shaping the shape of plants, from anther cracking to spread pollen, to fruit cracking and seed distribution, all are related to cells at the micro and macro levels "Separation" is closely related. Many kinds of fruits (such as siliques, capsules, follicles, pods, etc.) in the later stages of their development will undergo differentiation of special types of cells and a series of molecular and biochemical events closely related to them, eventually making mature The fruit cracks and releases the seeds, which is beneficial to expand the growth range of offspring plants, and provides conditions and guarantees for the prosperity of the race and the improvement of the adaptability...

AI Technical Summary

Problems solved by technology

Compared with obtaining non-dehiscence rapeseed lines by regulating transcription factors, non-dehiscence lines obtained by regulating downstream enzymes are more valuable for production and application, because transcription factors affect the entire pod dehiscence zone by regulating the expression of multiple genes Therefore, the strains produced by regulating the transcription factors will generally lead to the fruit not cracking at all, but in production, the cost will be increased due to the increased workload, which is not worth the loss compared with the loss caused by the reduced yield caused by cracking

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