Breeding method for multi-pod main inflorescence brassica oleracea type hybrid rape combination

A technique for multi-cornered main inflorescences of Brassica napus, which is applied in the fields of botany equipment and methods, applications, plant gene improvement, etc., can solve the problems of rising labor costs, declining rapeseed planting area, low degree of mechanization, etc., to increase the angle of main inflorescences The effect of fruit number

Active Publication Date: 2016-07-20
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, due to rising labor costs and low degree of mechanization, rapeseed acreage has declined year after year

Method used

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  • Breeding method for multi-pod main inflorescence brassica oleracea type hybrid rape combination
  • Breeding method for multi-pod main inflorescence brassica oleracea type hybrid rape combination
  • Breeding method for multi-pod main inflorescence brassica oleracea type hybrid rape combination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1, Breeding "Main Inflorescence Polygonal" Brassica napus Maintainer Line

[0054] 1. Discovery and breeding of "multi-horned main inflorescence" Brassica napus strain

[0055] In 2004, a mutant single plant with abnormally many horns in the main inflorescence was found in the breeding materials. After selfing and directional selection for many years, a new line 12R1402 with stable "multi-cornered main inflorescence" was bred from the offspring of the mutant strain. The maximum number of effective siliques in the main inflorescence of the strain is 300, with an average of about 180. A new strain 12R1402 with stable characteristics of "polygonal main inflorescence" was bred, and it was preserved in the General Microorganism Center of China Committee for Microbial Culture Collection (CGMCC for short, address: Beichen West Road, Chaoyang District, Beijing) on ​​April 8, 2016 No. 1 Courtyard No. 3, Institute of Microbiology, Chinese Academy of Sciences, Zip Code 10...

Embodiment 2

[0108] Example 2, Breeding "Main Inflorescence Polygonal" Cytoplasmic Male Sterile Line

[0109] 1. Based on the continuous backcrossing of the Qinyou 7 sterile line and the maintainer line "Main Inflorescence Duojiao" 202B, the "Main Inflorescence Duojiao" cytoplasmic male sterile line 202A was selected

[0110] Since 2009, according to image 3 For hybridization and backcrossing:

[0111] 1) hybridization

[0112] In March 2009, 10 sterile plants of Brassica napus Qinyou No. 7 (National Inspection Oil 2004014) were used as female parents, and 10 "Main Inflorescence Polygonal" 202B bred in Example 1 were used as male parents, and crossed to obtain 10 F 1 ; The male parent takes the powder plant and bags it for self-breeding.

[0113] 2) 1st backcross

[0114] In July 2009, from 10 hybrid progeny F 1 Select the single plant with the most flower buds in the main inflorescence (the number of flower buds in the main inflorescence is about twice that of Huyou 17), and record...

Embodiment 3

[0160] Embodiment 3, main inflorescence multi-corner hybrid combination breeding

[0161] 1. Breeding hybrid rape combination with multi-corner main inflorescence based on "multi-corner main inflorescence" 202A

[0162] 1. Breeding

[0163] In 2012 according to Figure 5 Perform combined screening:

[0164] In March 2012, using the 50 cytoplasmic male sterile line "Main Inflorescence Polygonal" 202A selected in Example 2 as the female parent, the Brassica napus restorer lines 7P76, 7P71 and 7P83 (7P76, 7P71 and 7P83 was 100% recovered by test crossing the fully fertile lines isolated from Qinyou 8 (Guo Shenyou 2004015), Huayouza 8 (Guo Shenyou 2004007), and Zhongyouza 8 (Guo Shenyou 2004004) The strains obtained through systematic breeding) were crossed with the male parent, and 3 hybrid combinations F 1 seed.

[0165] In October 2012, 3 hybrid combinations of 100 plants were planted; in May 2013, the hybrid combination F was identified 1 , more than 90% of the main infl...

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Abstract

The invention discloses a breeding method for a multi-pod main inflorescence brassica oleracea type hybrid rape combination. The breeding method comprises the following steps: (1) breeding of a multi-pod main inflorescence maintainer line and a sterile line: A) performing hybridization by taking a multi-pod main inflorescence rape CGMCC NO.12262 as a male parent and taking an ordinary maintainer line as a female parent, B) performing back-crossing by taking F1 as a non-recurrent parent and taking an ordinary maintainer line as a recurrent parent, and performing continuous back crossing to obtain the multi-pod main inflorescence maintainer line by taking a back-crossing offspring as a non-recurrent parent, and C) performing hybridization by taking an ordinary cytoplasm male sterile plant as a female parent and taking the multi-pod main inflorescence maintainer line as a male parent, and performing continuous back-crossing to obtain the multi-pod main inflorescence sterile line; (2) breeding of a multi-pod main inflorescence hybrid rape combination: performing hybridization on the multi-pod main inflorescence sterile line and an ordinary cytoplasm male sterile restorer line to breed the multi-pod main inflorescence hybrid rape combination. According to the method, the main inflorescence pod number of the hybrid rape combination can be remarkably increased, and new rape varieties suitable for light, simple and high-density planting can be screened more easily.

Description

technical field [0001] The invention belongs to the field of rapeseed breeding, and relates to a method for selecting a combination of main inflorescence polygonal cabbage hybrid rapeseed. Background technique [0002] Rapeseed is one of the three major oil crops in my country. The area and output of rapeseed in my country rank among the top in the world. In recent years, due to rising labor costs and low degree of mechanization, rapeseed acreage has declined year after year. Breeding rapeseed varieties suitable for light simplified cultivation and mechanized harvesting is one of the key measures to promote the restorative growth of rapeseed in my country. [0003] Academician Fu Tingdong proposed that one of the characteristics required for the selection of rapeseed varieties suitable for light simplified cultivation and mechanized harvesting is that they are suitable for dense planting. According to some studies, when planted at high density, the two agronomic traits of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 朱宗河郑文寅周可金陈莉黄界颖齐永霞
Owner ANHUI AGRICULTURAL UNIVERSITY
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