Preparation and application of biological adhesion enhanced temperature-sensitive chitosan-based postoperative adhesion prevention hydrogel
A sensitive chitosan-based, bioadhesive technology, applied in the fields of medicine and health, can solve the problems of short retention time, insufficient bioadhesion, difficult burden on patients, etc., and achieves difficult displacement or falling off, suitable degradation speed, and convenient injection and use. Effect
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[0100] Example 1, Hydroxybacly Synthesis
[0101] Alkalization: The chitosan 300 mg of the molecular weight of 1000 kD, 600 kD, 100 kd is placed in a 50 ml tapered bottle, and 50% (w / v) aqueous potassium hydroxide, stir, stir, sufficiently swelling for 2 h, then at room temperature Alkalization 24 h.
[0102] The isopropanol / water system is dispersed: alkalized chitosan filtration removes excess perikase and allows for 12 h at 4 ° C. 10 ml of isopropyl alcohol and 10 ml of deionized water were mixed with a water solution ratio of 1: 1, and the alkalized retained chitosan was added to the isopropyl alcohol solution of 1: 1, stirred at room temperature for 24 h, to base The chitosan is completely dispersed in the isopropyl alcohol / water system.
[0103] Grafting reaction: 20 ml of 1,2-cyclobutane is added dropwise to the isopropanol / water system dispersed with alkalized chitosan, and stirred while drying, and the reaction is stirred in a water bath at 60 ° C after drying. 24...
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[0106] Example 2, Hydroxybacly Based on Glycan
[0107] Weighing the appropriate amount of HBC with the KBR powder with the KBR powder with the KBR powder, load the sample cup, tablets, and the Fourier transform infrared spectrometer in 4000-400 cm. -1 Scan within the range. HBC's infrared spectrum such as figure 1 Indicated. 3400cm -1 Strong suction peaks overlapped with -N-H vibration absorption peaks are characteristic peaks of chitosan, and the chitosan characteristic peaks of the introduction of a hydroxybutyl group are preserved. 2870cm -1 -2980cm -1 The vibration absorption peak and 1463cm of three-C-H in the hydroxybutyl carbon chain -1 -CH introduced to the neat in the vicinity 3 The absorption peak is the characteristic peak of HBC. Due to the newly introduced hydroxyfrock group, at 1155cm -1 Nearby - C 6 -Oh's feature belt weakens and almost disappears. The HBC infrared spectrum prepared by different molecular weight chitosan demonstrates that the hydroxybacarium group ...
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[0108] Example 3, Hydroxybaclosan gel solution use concentration determination
[0109] The determination of the HBC phase change time is adopted using a "test tube method", i.e. On the desktop, the HBC in the 30S in-centrifuge tube does not take out along the tube wall, and the centrifuge tube has been soaked in the water bath when the centrifuge is taken out of the water bath. The temperature-sensitive gel phase change time, accurate to 5 s.
[0110] The HBC aqueous solution of 1.5% -5% (w / v) was prepared. According to the foregoing method, the case where different concentrations of HBC solution becomes gel in the 37 ° C water bath and recorded the phase change time, and the phase change time followed by concentration Curve, such as figure 2 Indicated. As the concentration of the HBC solution gradually increases, the phase change time at 37 ° C is gradually shortened, but the change amplitude is getting smaller and smaller, and there is a trend of balancing, and the concentrat...
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