Esophagus repairing material

A repair material and technology for esophagus, applied in medical science, prosthesis, etc., can solve problems such as insufficient mechanical strength, plastic difficulty, growth factor damage, insufficient supporting force, etc., and achieve good clinical application prospects, good histocompatibility, and rigidity big effect

Active Publication Date: 2015-01-07
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If this method is used to reconstruct the esophagus, there will still be defects such as insufficient support, easy collapse, and easy stenosis, which cannot solve the problems of insufficient mechanical strength and difficult plasticity, and because the dissolution of the small intestinal submucosa in organic solvents may lead to the destruction of growth factors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 Preparation of esophageal repair material of the present invention

[0046] 1. Preparation of SIS

[0047] The SIS of the present invention can be prepared by the following method, and commercially available products can also be used.

[0048] (1) Cleaning and finishing: take the fresh pig small intestine half an hour after slaughter, wash away the contents of the small intestine with water, turn over the small intestine, add salt and rub it, rinse it repeatedly with water for 3 times, cut open the small intestine with a scalpel, and then cut it into 15 cm long of the intestinal segment.

[0049] (2) Isolate the SIS: scrape off the muscular layer and serosa layer with a tongue depressor, and store in normal saline at 4°C overnight.

[0050] (3) Degreasing: Rinse with deionized water and drain the water with gauze. Immerse in the mixture of chloroform-methanol, the ratio of chloroform: methanol is 1:1, put it in the fume hood for 4 hours, change the liqui...

Embodiment 2

[0082] Example 2 Preparation and detection of long tubular SIS / PLGA-PHBHHx composite material

[0083] Take a 5cm stainless steel iron rod with a diameter of about 2mm, wet the SIS film prepared in Experimental Example 1 with water and wrap it around the outer edge of the iron rod for 2 laps, take 5ml of PHBHHx-PLGA mixed solution (5:5) and repeatedly cast it on the iron rod SIS For the outer layer of the film, the iron rod is continuously rotated during the casting process, and after it is solidified, it is freeze-dried. Gently take out the inner iron rod, and after the two ends are flattened, the tubular SIS / PLGA-PHBHHx composite material is obtained, such as Figure 7 a.

[0084] The bilayer structure of tubular composites after acridine orange (AO) staining was observed by confocal laser scanning microscopy, as Figure 7 As shown in B, the inner green ring is the double-layer SIS film, and the outer blue ring is the PHBHHx-PLGA composite.

[0085] The experimental resul...

experiment example 1

[0087] The detection of experimental example 1 SIS / PLGA-PHBHHx composite material of the present invention

[0088] Take the SIS / PLGA-PHBHHx (5:5) composite material prepared in Example 1 for the following tests:

[0089] (1) Determination of the release of VEGF and TGF-β growth factors in SIS / PLGA-PHBHHx composites by ELISA

[0090] Take 0.1g of the SIS film prepared in Example 1 and 3 groups of SIS / PHBHHx-PLGA composite materials prepared from 0.1g of the SIS film, soak in 3ml of PBS at 37°C under sterile conditions, and take out 1ml of the material extract every 48 hours. And add 1ml of fresh sterile PBS, continuous sampling for 30 days. The content of VEGF and TGF-β in the material extract was measured by ELISA method.

[0091] The result is as Figure 8 As shown, the SIS / PHBHHx-PLGA composite continuously releases VEGF and TGF-β growth factors. At 30 days, the cumulative release of VEGF reached 657ng / ml, and the cumulative release of SIS film was 811ng / ml; the cumulati...

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Abstract

The invention discloses an esophagus repairing material which is formed by compositing a polyhydroxybutyrate-co-hydroxyhexanoate-polylacide-co-glycolide-acid (PHBHHx-PLGA) material and small intestinal submucosa. The compound quantity of the PHBHHx-PLGA material is 0.001g-0.1g / cm2, the PHBHHx-PLGA material is a mixture of a polyhydroxybutyrate-co-hydroxyhexanoate copolymer and a polylacide-co-glycolide-acid copolymer, and the molar ratio of the polyhydroxybutyrate-co-hydroxyhexanoate copolymer and the polylacide-co-glycolide-acid copolymer is (3:7)-(7:3). The esophagus repairing material has the advantages that the mechanical property is excellent, the degradation speed is appropriate, the tissue compatibility and the cytocompatibility are good, the hemolysis is low, the material can be used for preparing artificial esophagus, and the material has an excellent applying prospect.

Description

technical field [0001] The invention relates to an organ repairing material, in particular to an esophageal repairing material. Background technique [0002] The esophagus is a long tubular muscular organ connecting the pharynx and the stomach, responsible for transporting food from the pharynx to the stomach. Due to eating habits, poor nutritional status, low intake of fruits and vegetables, excessive drinking, etc., my country is the country with the highest incidence and mortality of esophageal cancer, and the growth rate of the incidence rate far exceeds that of other cancers. Among the nearly 500,000 new patients with esophageal cancer every year in the world, more than half are from China. The number of deaths due to esophageal cancer in my country accounts for 11.16% of the total number of deaths due to malignant tumors, second only to lung cancer, liver cancer and gastric cancer. Esophageal obstruction caused by a variety of congenital diseases (such as long-term e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/18A61L27/36
Inventor 解慧琪范美荣龚梅
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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