A 3D printed antibacterial hydrogel repair scaffold and its preparation method

A 3D printing and hydrogel technology, applied in the field of biomedical engineering, can solve the problems of susceptibility to bacterial infection, lack of antibacterial properties, and single function of repairing scaffolds, and achieve suitable vascularization ability, good biocompatibility and antibacterial Performance, the effect of easy industrialization

Active Publication Date: 2020-03-31
广东省科学院生物与医学工程研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, 3D printed materials also need to meet certain mechanical properties in order to have good formability; in addition, the current repair scaffolds prepared by 3D printing have relatively single functions and do not have antibacterial properties, which often lead to their susceptibility to bacteria in applications. Infection leads to a series of inflammation and complications

Method used

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  • A 3D printed antibacterial hydrogel repair scaffold and its preparation method
  • A 3D printed antibacterial hydrogel repair scaffold and its preparation method
  • A 3D printed antibacterial hydrogel repair scaffold and its preparation method

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preparation example Construction

[0067] (1) Preparation of methacryloyl sodium hyaluronate: take a certain amount of sodium hyaluronate (1×10 6 ~1.2×10 6 kDa) was dissolved in distilled water to prepare a sodium hyaluronate solution with a mass concentration of 1%, and then adding a NaOH solution with a concentration of 5mol / L to adjust the pH of the sodium hyaluronate solution to 8.5; by mass ratio m sodium hyaluronate: m methacrylic anhydride = 1:7.8 Add methacrylic anhydride, react at 10°C for 24 hours, add it into a dialysis bag (molecular weight cut-off: 14,000) for dialysis with running water for 3 days, and freeze-dry at -80°C to obtain methyl Acrylylated sodium hyaluronate was stored in a -20°C refrigerator for later use.

[0068] (2) Preparation of methacrylated oxidized sodium alginate: dissolving sodium alginate in distilled water to prepare sodium alginate (5×10 6 ~8×10 6 kDa) solution, then add sodium periodate according to the mass ratio m sodium alginate: m sodium periodate=2:1, keep away fr...

Embodiment 1

[0080] The present invention prepares the antibacterial hydrogel repair support according to the above method, wherein: the molecular weight of the sodium hyaluronate used is 1×10 6 kDa; the molecular weight of sodium alginate is 5×10 6 kDa; in the preparation step of the composite printing precursor, the consumption of HA-MA is 20 parts by mass, the consumption of OMA is 20 parts by mass; the dopamine hydrochloride solution of 10mg / mL is 30 parts by volume; the consumption of antimicrobial peptide LL37 is 0.1 parts by mass, The concentration when used was 10 μg / mL; the HA-MA / OMA composite hydrogel treated with polydopamine surface modification was soaked in the antimicrobial peptide LL37 solution and placed in a sterile ultra-clean bench for 12 hours.

Embodiment 2

[0082] The present invention prepares the antibacterial hydrogel repair support according to the above method, wherein: the molecular weight of the sodium hyaluronate used is 1×10 6 kDa; the molecular weight of sodium alginate is 5×10 6 kDa; in the preparation step of the composite printing precursor, the consumption of HA-MA is 20 parts by mass, the consumption of OMA is 40 parts by mass; the dopamine hydrochloride solution of 10mg / mL is 30 parts by volume; the consumption of antimicrobial peptide LL37 is 0.1 parts by mass, The concentration when used was 30 μg / mL; the HA-MA / OMA composite hydrogel treated with polydopamine surface modification was soaked in the antimicrobial peptide LL37 solution and placed in a sterile ultra-clean bench for 18 hours.

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Abstract

The invention discloses a 3D printed antibacterial hydrogel repairing support and a preparation method thereof. The preparation method comprises the following steps: preparing a composite hydrogel support by carrying out photocuring 3D printing on modified sodium hyaluronate / modified oxidized sodium alginate hydrogel precursor, and then modifying an antibacterial peptide LL-37 on the surface of the composite hydrogel support, so that the 3D printed antibacterial hydrogel repairing support is obtained. The preparation method provided by the invention is simple, short time is consumed, industrialization can be easily realized, and the size and geometrical shape of the support can be customized according to a damaged part. The support has the compression strength of 0.16-0.29MPa, can effectively inhibit Escherichia coli and staphylococcus aureus and has good cell compatibility.

Description

technical field [0001] The invention belongs to the technical field of biomedical engineering, in particular to a 3D printed antibacterial hydrogel repair bracket and a preparation method thereof. Background technique [0002] Some soft tissues, such as cartilage tissue and adipose tissue, do not have the ability to regenerate because they do not contain or have few blood vessels and lymph. And because its self-repair ability is very limited, when the soft tissue is damaged or missing, it cannot repair itself. Tissue-engineered scaffolds are an important alternative therapeutic measure that promises to finally address this issue. Since hydrogel can maintain a fluid state under certain conditions and form a material with a certain shape and strength under external physical or chemical stimuli, this intelligence can be used to prepare injection-type scaffolds, and play its role in repairing complex-shaped defects. In addition, the hydrogel scaffold can provide a microenviron...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/50A61L27/52A61L27/58A61L27/54A61L27/20A61L27/22B33Y10/00
CPCA61L27/20A61L27/227A61L27/50A61L27/52A61L27/54A61L27/58A61L2300/252A61L2300/404A61L2430/34B33Y10/00C08L5/08C08L5/04C08L89/00
Inventor 马年方梁磊曾建李锦荣郭剑雄王庆福康佩姿
Owner 广东省科学院生物与医学工程研究所
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