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Preparation method of bionic cartilage extracellular matrix for tissue engineering

A chondrocyte and tissue engineering technology, which is applied in the field of preparation of bionic cartilage extracellular matrix for tissue engineering, can solve problems such as differences, and achieve the effect of simple and easy process, good reproducibility, and less clinical complications

Active Publication Date: 2010-04-07
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2004, the Chinese Armed Police General Hospital applied the tissue engineered cartilage constructed by Verigen in clinical practice, but my country still lacks tissue engineered cartilage matrix materials with independent intellectual property rights for clinical application
There are significant differences between the tissue engineered cartilage scaffold material prepared by the prior art and the normal cartilage matrix in terms of composition and structure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation method of the bionic cartilage extracellular matrix for tissue engineering of the present embodiment comprises the following steps:

[0032]Dissolve type II collagen, chondroitin sulfate and hyaluronic acid in 0.05mol / L malonic acid solution according to the mass percentage of 6:3:1, stir well to make it completely dispersed, and prepare the total mass concentration of bionic raw materials as 15% Acidic collagen mixture; according to the principles of tissue engineering, select NaCl crystals with a particle size of 125-150 μm as the pore-forming agent; take 100ml of acidic collagen mixture, add 125-150 μm particle size at 20°C 100g of NaCl crystals, fully stirred to make it evenly mixed; take 10ml of acidic collagen mixture containing NaCl and inject it into a cylindrical mold with a diameter of 3cm for making bionic cartilage extracellular matrix for tissue engineering, pressurize, seal and seal the mold. Place in a -10°C refrigerator for 4 hours; then ...

Embodiment 2

[0039] The preparation method of the bionic cartilage extracellular matrix for tissue engineering of the present embodiment comprises the following steps:

[0040] Dissolve type II collagen, chondroitin sulfate and hyaluronic acid in 0.05mol / L malonic acid solution according to the mass percentage of 7:2.5:0.5, stir well to make it completely dispersed, and prepare the total mass concentration of bionic raw materials 5% acidic collagen mixture; then according to the principles of tissue engineering, select KCl crystals with a particle size of 100-125 μm as the pore-forming agent; then add 100ml of acidic collagen mixture, at 25°C, add a particle size of 120g of KCl crystal particles of 100-125μm, fully stirred to mix evenly; take 10ml of acidic collagen mixture containing KCl and inject it into a cylindrical mold with a diameter of 3cm, pressurize the mold, seal it and place it in a refrigerator at -20°C placed in the refrigerator for 2 hours; then placed the mold in a -80°C r...

Embodiment 3

[0044] The preparation method of the bionic cartilage extracellular matrix for tissue engineering of the present embodiment comprises the following steps:

[0045] Dissolve type II collagen, chondroitin sulfate and hyaluronic acid in 0.1mol / L acetic acid solution according to the mass percentage of 8:1.5:0.5, stir well to make them completely dispersed, and prepare the bionic raw materials with a total mass concentration of 30 % acidic collagen mixture; then according to the pore size design requirements of the bionic cartilage extracellular matrix for tissue engineering, NaCl crystals with a particle size of 120-140 μm were selected as the pore-forming agent; Next, add 90g of NaCl crystal particles with a particle size of 120-140μm, stir well to make it evenly mixed; take 10ml of acidic collagen mixture containing NaCl and inject it into a cylindrical mold with a diameter of 3cm, pressurize the mold, seal it and place it Place in a -30°C refrigerator for 3 hours; then place t...

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PUM

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Abstract

The invention discloses a preparation method of a bionic cartilage extracellular matrix for tissue engineering, which comprises the following steps of: selecting a raw material having the same main components as the normal articular cartilage extracellular matrix as a bionic raw material to prepare the bionic cartilage extracellular matrix for the tissue engineering; fully dissolving the selectedbionic raw material in organic acid; and then carrying out hole making processing, curing processing and strengthening processing in sequence to the selected bionic raw material. The method makes thematerial structure of the finally obtained bionic cartilage extracellular matrix for the tissue engineering have physical structure characteristics suitable for biological behavior requirements such as vaccination, survival, multiplication and the like of a cartilage seed cell and have good biocompatibility, appropriate degradation rate and biomechanical strength at the same time. The preparationmethod has the advantages of wide material source, low cost, simple and easy process and good reproducibility, and the prepared product can be widely used to repair a large area of articular cartilage defect and has fewer clinical complications.

Description

technical field [0001] The invention relates to cartilage bionic extracellular matrix, in particular to a preparation method of bionic cartilage extracellular matrix for tissue engineering. Background technique [0002] Cartilage defects in joints caused by trauma or bone disease are common clinically, seriously affect the quality of life of patients, and have become one of the main causes of physical disabilities. The incidence rate in the United States is 1.5‰~3‰, and in my country it is about 5 to 6 times that of the United States, and it is increasing year by year. Articular cartilage belongs to hyaline cartilage, lacks neurovascular nutrition, and is difficult to repair itself after injury. There are obvious defects in the existing clinical treatment measures. The conservative treatment and joint debridement can only temporarily relieve the pain, but cannot prevent the development of the disease. defect; allogeneic osteochondral transplantation has the possibility of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/56A61L27/58
Inventor 张颖王富友崔运利杨柳
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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