Composite biomaterial and preparation method and application thereof

A technology of composite biomaterials and derivatives, applied in the field of medicine, can solve problems such as poor fatigue resistance, poor degradability, and easy fracture, and achieve strong mechanical properties, good biocompatibility, and safe use

Inactive Publication Date: 2009-12-09
INST OF BIOPHARM OF SHANDONG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

HAP has good biocompatibility and osteoconduction, but its anti-fatigue strength is poor, brittle, easy to break, and poor in degradation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Composite biomaterial composed of 1,4-butanediol diglycidyl ether (BDDE) cross-linked cHA gel and HAP. The preparation method is as follows:

[0025] Method 1.1: (1) Mix 1g of sodium hyaluronate (SH, with an average molecular weight of 1 million Daltons) and 0.25g of HAP (average particle size of 100-500μm), and then mix it with 5ml of 1% NaOH solution containing 50mg of BDDE , reacted at 50°C for 2-4h, added 40ml of distilled water, and stood at 25°C for 8-16h to obtain a block gel. (2) Freeze-dry the bulk gel for 6-12 hours, purify it with physiological saline for 10 hours, dehydrate it with ethanol, dry it under reduced pressure, cut or crush it, and sterilize it with ethylene oxide to obtain the product.

[0026] Method 1.2: (1) According to the step (1) in method 1.1 to obtain block gel. (2) Add an equal volume of 1.8% sodium chloride solution to soak the block gel for 4 hours, then purify it with physiological saline for 4-6 hours, cut or crush it, freeze-dry it...

Embodiment 2

[0037] Composite biomaterial composed of adipate dihydrazide (ADH) cross-linked cHA gel and HAP. The preparation method is as follows:

[0038] Method 2.1: (1) Dissolve 1.25g SH (average molecular weight 1.2 million Daltons) in 50ml distilled water, add ADH 125mg and ethyl (N,N-dimethylaminopropyl) carbodiimide (EDCI) 0.3g, add 0.5mol / L hydrochloric acid solution dropwise to adjust the pH value to 6.0, stir for 4 hours, add an equal volume of PBS with 2 times the isotonic concentration and soak for 4 hours, then purify with isotonic PBS for 4-6 hours, and break to obtain gel particles. (2) Add 0.05g of HAP (average particle size: 10-100μm) and mix evenly, and sterilize with circulating steam at 100°C for 30 minutes to obtain the product.

[0039] Method 2.2: (1) Obtain gel particles according to step (1) in method 2.1. (2) Add 10 g of HAP (average particle size 0.1-10 μm), mix well, and sterilize at 121° C. for 15 minutes to obtain the product.

[0040] Method 2.3: (1) Diss...

Embodiment 3

[0047] Composite biomaterial composed of divinyl sulfone (DVS) cross-linked cHA gel and HAP. The preparation method is as follows:

[0048] Method 3.1: (1) Mix 1g SH (average molecular weight 1,000,000 Daltons) and 0.25g HAP (average particle size 100-500μm), mix with 25ml of 1% NaOH solution containing 0.2g DVS, and store at 25°C After reacting for 1 hour, add 25ml of distilled water and let it stand at 25°C for 8-12 hours to obtain block gel. (2) Freeze-dry the bulk gel for 6-12 hours, purify it with physiological saline for 10 hours, dehydrate it with ethanol, dry it under reduced pressure, cut or crush it, and sterilize it with ethylene oxide to obtain the product.

[0049] Method 3.2: (1) According to the step (1) in method 3.1 to obtain block gel. (2) Purify the block gel with 50% to 70% ethanol for 4 to 12 hours, dehydrate with absolute ethanol, dry under reduced pressure, cut or crush, and sterilize with ethylene oxide to obtain the product.

[0050] Method 3.3: (1)...

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Abstract

The invention relates to a composite biomaterial containing cross-linked hyaluronic acid derivative and hydroxylapatite and a preparation method thereof. The composite biomaterial is characterized by containing the cross-linked hyaluronic acid derivative and the hydroxylapatite. The composite biomaterial can be obtained by uniformly mixing hyaluronic acid or soluble salt of the hyaluronic acid with the hydroxylapatite and then carrying out the cross-linked reaction, or can be obtained by firstly preparing the cross-linked hyaluronic acid derivative and then uniformly mixing the cross-linked hyaluronic acid derivative with the hydroxylapatite. The composite biomaterial has good biological compatibility, has suitable rate of biodegradation, mechanical property, pore diameter and porosity, and can be used as the cytoskeleton to be applied to bone or cartilage tissue engineering.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a composite biological material and its preparation method. More specifically, the present invention relates to a composite biomaterial containing cross-linked hyaluronic acid derivatives and hydroxyapatite, its preparation method and application. Background technique [0002] The metabolic activity of articular cartilage tissue is low, and the damage is difficult to repair itself, which can eventually cause the degeneration of the articular surface. Surgical methods such as articular chondroplasty, microfracture, and autologous cartilage transplantation can temporarily improve the patient's clinical symptoms, but due to problems such as insufficient donor sources, immune rejection, poor cartilage formation, and poor long-term results, it is far from possible. Meet the needs of clinical application. The use of tissue engineering to repair cartilage defects has the advantages of requiring ...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61F2/82
Inventor 凌沛学陈建英贺艳丽汪敏刘杰荣晓花
Owner INST OF BIOPHARM OF SHANDONG PROVINCE
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