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Method for preparing HA/collagen/chitosan interpenetrating polymer network bracket

A technology of interpenetrating polymers and chitosan, applied in medical science, prostheses, etc.

Inactive Publication Date: 2009-12-02
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no precedent for the IPN technology used in the preparation of HA / collagen / chitosan interpenetrating polymer network bone tissue engineering scaffolds. The hydroxyapatite in the bone tissue engineering scaffold of the Internet of Things is uniformly dispersed, and the directional arrangement of the hydroxyapatite particles in the matrix and the formation of effective bonds with the matrix are expected to solve the existing problems of collagen / hydroxyapatite and On the basis of the existing problems of chitosan / hydroxyapatite composite scaffolds, the stability and mechanical properties of scaffold materials can be improved, and it is also expected to realize the effective composite of nano-hydroxyapatite and matrix from the structure and function

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A preparation method of HA (nano hydroxyapatite) / collagen / chitosan interpenetrating polymer network scaffold comprises the following steps and process conditions thereof:

[0046] Step 1: Preparation of HA sol

[0047] (1) Use polyvinylpyrrolidone (PVP K15) as template agent, dispersant and modifier at the same time;

[0048] (2) Use deionized water to prepare a solution containing 0.01mol / L of calcium nitrate and 0.006mol / L of diammonium hydrogen phosphate, and then add 0.1g of PVP K15 to the above calcium nitrate solution to fully dissolve;

[0049] (3) Using a high-speed disperser at 100 rpm, under the condition that the pH value of the ammonia water control system is 10.6, the materials in the above (1) and (2) are fully mixed to form a nano-hydroxyapatite sol, and the nano-hydroxyapatite sol is made. The molar ratio of calcium to phosphorus in the hydroxyapatite sol system reaches 1.6:1, and the content of polyvinylpyrrolidone is 0.01% by mass percentage;

[0050...

Embodiment 2

[0063] A preparation method of HA / collagen / chitosan interpenetrating polymer network scaffold comprises the following steps and process conditions thereof:

[0064] Step 1: Preparation of HA sol

[0065] (1) Use polyvinylpyrrolidone (PVP K30) as template agent, dispersant and modifier at the same time;

[0066] (2) Use the PVP K30 aqueous solution of mass percent 0.5% to prepare the solution containing calcium nitrate 0.1mol / L, disodium hydrogen phosphate 0.06mol / L;

[0067] (3) adopting a high-speed disperser of 1000 rpm, under the condition that the pH value of the sodium hydroxide control system is 11, the materials of the above-mentioned (1), (2) are fully mixed to form nano-hydroxyapatite sol, Make the molar ratio of calcium and phosphorus in the nano-hydroxyapatite sol system reach 1.65:1, and the content of polyvinylpyrrolidone by mass percentage is 0.5%;

[0068] Step 2: Aging of HA sol

[0069] Move the above-mentioned hydroxyapatite sol into a heating evaporation ...

Embodiment 3

[0081] A preparation method of HA / collagen / chitosan interpenetrating polymer network scaffold comprises the following steps and process conditions thereof:

[0082] Step 1: Preparation of HA sol

[0083] (1) Use polyvinylpyrrolidone (PVP K60) as template agent, dispersant and modifier at the same time;

[0084] (2) use the PVP K60 aqueous solution of 5% by mass percentage to prepare the solution containing calcium nitrate 0.5mol / L, dipotassium hydrogen phosphate 0.3mol / L;

[0085] (3) adopt the high-speed disperser of 2000 revs / min, under the condition that potassium hydroxide control system pH value is 11.5, the material of above-mentioned (1), (2) is fully mixed, forms nano-hydroxyapatite sol, makes The molar ratio of calcium to phosphorus in the nano-hydroxyapatite sol system reaches 1.67:1, and the polyvinylpyrrolidone content is 5% by mass percentage;

[0086] Step 2: Aging of HA sol

[0087] Move the above-mentioned hydroxyapatite sol into a heating evaporation reflux...

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Abstract

The invention discloses a method for preparing an HA / collagen / chitosan interpenetrating polymer network bracket. The method comprises the following steps: firstly, forming an HA sol system containing polyvinylpyrrolidone in an alkali liquor control system, aging and washing the HA sol system to obtain granular directional arranged HA sol, mixing the HA sol and blended solution of collagen and chitosan, dispersing the mixture by adopting a high-speed dispersion machine to obtain mixed solution of HA / collagen / chitosan, reducing pressure and removing foam from the mixed solution which is crosslinked by mixed cross linker and chitosan cross linker, injecting the mixed solution into a model, and then carrying out post treatment to obtain the bracket. The bracket is compounded with a matrix in a form of HA sol, and the HA in granular directional arrangement has good dispersion in the collagen / chitosan interpenetrating polymer network matrix and forms effective and stable bond with the matrix so as to realize the effective compound of the HA and the collagen / chitosan matrix on the structure and function. The method also effectively solves the problems of collagen and chitosan materials at the same time. The method has simple process and simple operation, and is easy for popularization and application.

Description

technical field [0001] The invention relates to the technical field of artificial bone repair materials, in particular to a preparation method of an HA (nano-hydroxyapatite) / collagen / chitosan interpenetrating polymer network scaffold. technical background [0002] Clinically, there are a large number of patients with hard tissue defects caused by trauma, tumors and other diseases every year. Therefore, hard tissue repair materials are one of the most clinically demanded repair materials at home and abroad. In recent years, due to some problems existing in traditional bone repair materials, bone tissue engineering scaffolds have become a research hotspot. Cultivate and construct bionic bone with vitality. [0003] Collagen, chitosan and hydroxyapatite are widely used as scaffold materials for bone tissue engineering due to their good biocompatibility and other characteristics. Based on the fact that the main components of natural bone are collagen and hydroxyapatite, collag...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/56A61L27/12A61L27/24A61L27/20
Inventor 宁成云王迎军尹兆益魏坤张德贵
Owner SOUTH CHINA UNIV OF TECH
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