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Collagen/chitosan three-dimensional network artificial cornea scaffold and preparation method thereof

A three-dimensional, artificial cornea technology, used in stents, prostheses, medical sciences, etc., can solve the problems of no antigenicity, fast degradation, low mechanical strength, etc., and achieve the effects of simple operation, simple preparation process, and easy popularization and application.

Inactive Publication Date: 2009-12-09
SCHOOL OF OPHTHALMOLOGY & OPTOMETRY WENZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen has no antigenicity, good tissue compatibility, and contains certain specific amino acid sequences, which is conducive to the adhesion and growth of seed cells. The disadvantages of collagen are poor stability, low mechanical strength, and rapid degradation
The three-dimensional network scaffold is a new type of multi-phase polymer material that uses chemical methods to interpenetrate two or more polymers into an interwoven network. It is a new field for the development of polymer blending modification technology. It has opened up a new way. At present, there is no precedent for the three-dimensional network technology to be used in the preparation of collagen / chitosan artificial corneal tissue engineering scaffolds. In the scaffold, the stability and mechanical properties of the scaffold material can be improved, and it is also expected to realize the effective compounding of multiphase polymers in terms of structure and function.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) 5 ooml of collagen solution of 3mg / ml is prepared with the acetic acid solution of 2% with mass percent concentration;

[0020] (2) join chitosan in the collagen solution of above-mentioned (1) in the ratio of 0.1: 1 by chitosan: the mass ratio of collagen solute, adopt high-speed disperser to obtain collagen after fully mixing with the speed of 100 rpm / chitosan blend;

[0021] (3) Add a mixed cross-linking agent with a total content of 0.01% by mass percentage in the collagen / chitosan blend of the above (2), stir and cross-link for 12 hours at room temperature, and add 1-B in the mixed cross-linking agent The mass ratio of base-3-(3-dimethylaminopropyl) carbodiimide (EDC) to N-hydroxysuccinimide (NHS) is 1:1;

[0022] (4) Add a 0.01% glutaraldehyde solution by mass percentage to the above (3) collagen / chitosan mixture cross-linked by EDC+NHS and stir and cross-link at room temperature for 12 hours;

[0023] (5) The collagen / chitosan mixture finally obtained in ...

Embodiment 2

[0025] (1) 500ml of collagen solution of 4mg / ml is prepared with the acetic acid solution of 2% with mass percent concentration;

[0026] (2) Chitosan is added in the collagen solution of above-mentioned (1) in the ratio that the mass ratio of chitosan: collagen solute is 0.25: 1, adopts high-speed disperser to obtain collagen after fully mixing with the speed of 1000 rpm / chitosan blend;

[0027] (3) Add a mixed cross-linking agent with a total content of 0.05% by mass percentage in the collagen / chitosan mixture of the above (2), stir and cross-link for 12 hours at room temperature, and add 1-B in the mixed cross-linking agent The mass ratio of base-3-(3-dimethylaminopropyl) carbodiimide (EDC) to N-hydroxysuccinimide (NHS) is 2:1;

[0028] (4) Add polyethylene glycol bisglycidyl ether with a content of 0.05% by mass percentage in the mixed solution of collagen / chitosan cross-linked by EDC+NHS in the above (3) and stir and cross-link at normal temperature 12h;

[0029] (5)...

Embodiment 3

[0031] (1) 500ml of collagen solution of 5mg / ml is prepared with the acetic acid solution of 2% with the mass percent concentration;

[0032] (2) join chitosan in the collagen solution of above-mentioned (1) in the ratio of 0.65: 1 by chitosan: the mass ratio of collagen solute, adopt high-speed disperser to obtain collagen after fully mixing with the speed of 2000 rpm / chitosan blend;

[0033] (3) Add a mixed cross-linking agent with a total content of 0.1% by mass percentage in the collagen / chitosan mixture of the above (2), stir and cross-link for 12 hours at room temperature, and add 1-B in the mixed cross-linking agent The mass ratio of base-3-(3-dimethylaminopropyl) carbodiimide (EDC) to N-hydroxysuccinimide (NHS) is 4:1;

[0034] (4) In the above (3) mixed solution of collagen / chitosan cross-linked by EDC+NHS, add polyethylene glycol bisglycidyl ether with a content of 0.1% by mass percentage and stir and cross-link at normal temperature 12h;

[0035] (5) The collag...

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Abstract

The invention provides a method for preparing collagen / chitosan three-dimensional network artificial cornea scaffold, comprising: taking acetic acid solution as solvent as well as collagen and chitosan as solute for preparing collagen / chitosan mixed solution; adding 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide and N-hydroxy succinimide mixed crosslinking agent into the mixed solution; after that, adding chitosan crosslinking agent; finally, removing bubble and injecting the obtained mixed solution into a model, putting the model into an ultra low temperature refrigerator to be frozen and transferring into a freezer dryer for freeze drying, and obtaining the collagen / chitosan three-dimensional network artificial cornea scaffold.

Description

Technical field: [0001] The invention relates to the technical field of artificial cornea materials, in particular to a collagen / chitosan three-dimensional network artificial cornea support and a preparation method thereof. Background technique: [0002] Corneal disease is the second most common cause of blindness next to cataract. Corneal transplantation is currently an effective method for treating corneal blindness, but it faces problems such as severe shortage of corneal donors, postoperative immune rejection and iatrogenic astigmatism. With the development and progress of tissue engineering technology, tissue engineering cornea will open up new ways for the treatment of corneal diseases. The core of constructing tissue engineering cornea to replace partial or full-thickness damaged cornea is to establish a three-dimensional space complex of cells and biomaterials. , the ideal scaffold material has always been a bottleneck problem restricting the development of tissue en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/56A61F2/82A61L27/24A61L27/20
Inventor 陈浩瞿佳
Owner SCHOOL OF OPHTHALMOLOGY & OPTOMETRY WENZHOU MEDICAL COLLEGE
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