Biological composite scaffold for repairing bone defect and preparation method thereof

A composite scaffold and bone defect technology, applied in medical science, prosthesis, etc., can solve problems such as lack of systematic research, and achieve the effect of easier control of porosity and pore size distribution, and good mechanical properties

Inactive Publication Date: 2010-12-01
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation of SF / HA composite scaffolds and related in vivo and in vitro experiments are still in their infancy, and there is a lack of systematic research.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Put the mulberry silk in 0.02mol / L sodium carbonate solution and boil it for 30 minutes, then wash it with deionized water and dry it to remove the sericin protein that is sensitized to human tissues;

[0020] (2) dissolving the above degummed silk fibroin in a 9.3mol / LLiBr solution and performing dialysis at room temperature to obtain an aqueous silk fibroin solution;

[0021] (3) Calculate the required Ca(NO 3 ) 2 and (NH 4 ) 2 HPO 4 The mass; weigh the required mass of Ca(NO 3 ) 2 , add it to the above silk fibroin aqueous solution and mix to obtain SF-Ca(NO 3 ) 2 solution;

[0022] (4) Weigh the corresponding mass (NH 4 ) 2 HPO 4 , prepared into an aqueous solution with a concentration of 2mol / L; it was added dropwise to the above SF-Ca(NO 3 ) 2 In the mixed solution, ammonia water is added during the process to adjust the pH value to keep it at 10;

[0023] (5) Wash the above-mentioned precipitate to neutrality, filter to obtain the SF / nano HA com...

Embodiment 2

[0026] (1) Put the mulberry silk in 0.02mol / L sodium carbonate solution and boil it for 30 minutes, then wash it with deionized water and dry it to remove the sericin protein that is sensitized to human tissues;

[0027] (2) dissolving the above degummed silk fibroin in a 9.3mol / L LiBr solution and performing dialysis at room temperature to obtain an aqueous silk fibroin solution;

[0028] (3) Calculate the required Ca(NO 3 ) 2 and (NH 4 ) 2 HPO 4 The mass; weigh the required mass of Ca(NO 3 ) 2 , add it to the above silk fibroin aqueous solution and mix to obtain SF-Ca(NO 3 ) 2 solution;

[0029] (4) Weigh the corresponding mass (NH 4 ) 2 HPO 4 , prepared into an aqueous solution with a concentration of 2mol / L; it was added dropwise to the above SF-Ca(NO 3 ) 2 In the mixed solution, ammonia water is added during the process to adjust the pH value to keep it at 10;

[0030] (5) Wash the above-mentioned precipitate to neutrality, filter to obtain the SF / nano HA co...

Embodiment 3

[0033](1) Put mulberry silk in 0.02mol / L sodium carbonate solution and boil for 30 minutes, then wash and dry with deionized water, and remove sericin that has sensitization to human tissues;

[0034] (2) dissolving the above degummed silk fibroin in a 9.3mol / L LiBr solution and performing dialysis at room temperature to obtain an aqueous silk fibroin solution;

[0035] (3) Calculate the required Ca(NO 3 ) 2 and (NH 4 ) 2 HPO 4 The mass; weigh the required mass of Ca(NO 3 ) 2 , add it to the above silk fibroin aqueous solution and mix to obtain SF-Ca(NO 3 ) 2 solution;

[0036] (4) Weigh the corresponding mass (NH 4 ) 2 HPO 4 , prepared into an aqueous solution with a concentration of 2mol / L; it was added dropwise to the above SF-Ca(NO 3 ) 2 In the mixed solution, ammonia water is added during the process to adjust the pH value to keep it at 10;

[0037] (5) Wash the above-mentioned precipitate to neutrality, filter to obtain the SF / nano HA composite sol; add NaC...

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PUM

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Abstract

The invention discloses a biological composite scaffold for repairing bone defect. The composite scaffold is prepared from nano hydroxyapatite and silk fibroin, wherein the mass ratio of the nano hydroxyapatite to the silk fibroin is 90:10-60:40. The composite scaffold is porous, the porosity is 70 to 95 percent, the aperture size is 100 to 600 microns, pores are mainly circular, and the pores are mutually communicated. Aqueous solution of the de-gummed silk fibroin is used as a carrier, silk fibroin/nano hydroxyapatite composite sol is prepared by a co-precipitation method, the silk fibroin/nano hydroxyapatite composite scaffold is prepared by combining ion percolation and freeze drying process, and the distribution of aperture in the composite scaffold is controlled by adjusting the radius of NaCl particles and freezing parameters. Compared with the prior art, the scaffold has the advantages of easily-controlled porosity and the aperture distribution, and good mechanical property.

Description

technical field [0001] The invention relates to a biocomposite scaffold and a preparation method thereof, in particular to a silk fibroin / nanometer hydroxyapatite composite scaffold and a preparation method thereof, belonging to the field of biomedical materials. Background technique [0002] Bone tissue defect caused by trauma, tumor, congenital deformity, infection, pathology and other factors is one of the clinical problems. At present, the clinically used bone repair materials have their own advantages and disadvantages, and cannot meet the ideal requirements. The key issue to solve this problem is to develop biocompatible and degradable three-dimensional porous scaffold materials. [0003] Hydroxyapatite (HA) is the main inorganic component of natural bone tissue, has good biocompatibility, and has been widely used in the repair of various bone defects. However, the material prepared from micron-sized hydroxyapatite powder has low brittleness, low strength, and poor b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/22A61L27/12A61L27/56
Inventor 赵冬梅卢志华李鹏王海斌
Owner SHANDONG UNIV
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