The invention discloses a preparation method for a natural basin-based bionic structure bone scaffold. Silk protein and wool protein are taken as main raw materials, a silk protein solution and glycerinum are uniformly mixed according to the mass ratio of 60-120:20-60 and injected in a bone scaffold mold, a wool keratin solution is slowly injected into the mixed solution, and then through -40--20DEG C freezing and -72-5 DEG C vacuum freezing drying under 1.0*105-1.0 Pa, the silk protein/wool keratin bionic bone scaffold material is obtained. The prepared scaffold material is similar to the autogenous bone structure, the porosity rate reaches 70-95%, the hole diameter is uniform in distribution, the outer layer is compact, the hole diameter is smaller, the diameter range is 30-150 microns,the scaffold material has certain mechanical supporting performance, the diameter of inner holes is larger, the diameter range is 250-600 microns, and the scaffold material is suitable for migration,dependence and proliferation of cells.