Preparation method for natural basin-based bionic structure bone scaffold

Abstract

The invention discloses a preparation method for a natural basin-based bionic structure bone scaffold. Silk protein and wool protein are taken as main raw materials, a silk protein solution and glycerinum are uniformly mixed according to the mass ratio of 60-120:20-60 and injected in a bone scaffold mold, a wool keratin solution is slowly injected into the mixed solution, and then through -40--20DEG C freezing and -72-5 DEG C vacuum freezing drying under 1.0*105-1.0 Pa, the silk protein/wool keratin bionic bone scaffold material is obtained. The prepared scaffold material is similar to the autogenous bone structure, the porosity rate reaches 70-95%, the hole diameter is uniform in distribution, the outer layer is compact, the hole diameter is smaller, the diameter range is 30-150 microns,the scaffold material has certain mechanical supporting performance, the diameter of inner holes is larger, the diameter range is 250-600 microns, and the scaffold material is suitable for migration,dependence and proliferation of cells.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a preparation method of a natural protein-based biomimetic structure bone scaffold. Background technique [0002] Bone defect diseases caused by factors such as congenital defects or infection, trauma and tumor resection have been plagued by tens of millions of patients in the world. Traditional surgical treatment methods and radiotherapy and chemotherapy methods are usually difficult to effectively achieve the purpose of treatment. Organ transplantation is not only expensive and has few donor resources, but also has the risk of disease transmission and cross-infection, so it cannot be used as a routine treatment. In recent years, the development of tissue engineering materials has provided a new and effective way to treat such diseases. Among them, scaffold materials for bone defect repair have become one of the most clinically demanded biomedical mater...

AI Technical Summary

Problems solved by technology

However, the common problems of these materials are that the growth of new bone is limited by the local microenvironment, and the crawling replacement is slow. When treating large-scale bone defects, it is difficult to achieve the integration of the scaffold and bone and the complete repair and reconstruction of the defect area.

The repair and regeneration of bone defects requires a suitable external microenvironment. If the internal framework structure of the material cannot simulate the network structure formed by the extracellular matrix of autologous bone, it is not conducive to the communication and information transmission between cells, and the growth and migration of cells involved in bone repair. Limited, difficult to achieve osseointegration

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