Scaffolds of umbilical cord decellularized Wharton jelly for tissue engineering and preparation method thereof

A tissue engineering and decellularization technology, applied in medical science, prostheses, etc., can solve the problems of product acidity, unfavorable cell adhesion, easy to cause inflammation, etc., and achieve low cost, good repeatability, and good biophase capacitive effect

Inactive Publication Date: 2011-09-28
卢世璧
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Problems solved by technology

The disadvantages of artificial synthetic materials are: hydrophobicity, which is not conducive to cell adhesion, the product is acidic when degraded, and it is easy to cause inflammation in

Method used

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  • Scaffolds of umbilical cord decellularized Wharton jelly for tissue engineering and preparation method thereof
  • Scaffolds of umbilical cord decellularized Wharton jelly for tissue engineering and preparation method thereof
  • Scaffolds of umbilical cord decellularized Wharton jelly for tissue engineering and preparation method thereof

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Embodiment Construction

[0040] The present invention is further described through examples below, it should be understood that these examples are for illustration purposes only, and in no way limit the scope of the present invention.

[0041] 1. Preparation method

[0042] The umbilical cord decellularized Wharton glue three-dimensional porous sponge scaffold was prepared according to the following steps:

[0043] 1. Take the human umbilical cord, peel off the umbilical cord adventitia and vascular tissue (two arteries, one vein) under sterile conditions, cut the remaining tissue into pieces (1-2cm), rinse with distilled water for 3 times, and then soak in three distilled water Place at -10°C, hypotonic freeze-thaw for 24 hours, then soak and stir with 1% hydrogen peroxide for 5-10 minutes, then rinse with distilled water 3-5 times, the volume ratio of hydrogen peroxide to tissue is 2:1, the purpose is to make the tissue bulky , which is conducive to the destruction and detachment of cells in the ti...

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Abstract

The invention discloses scaffolds of umbilical cord decellularized Wharton jelly for tissue engineering and a preparation method thereof. Umbilical cords are employed as the raw material and their outer membranes and vascular tissues are peeled off. And the rest part of the umbilical cords is subjected to hypotonic freeze-thaw, mechanical pulverization, differential centrifugation, enzymatic digestion for decellularization. Then the umbilical cord Wharton jelly is collected and injected into a mold. After freeze drying and crosslinking, multiple three dimensional porous sponge scaffolds and composite scaffolds can be obtained. The method of the invention has the advantages of wide material source, low cost, simple technology. And the prepared scaffolds are characterized by controllable fine structure, appropriate degradation rate, good biocompatibility, and biomechanical strength, which are in favor of cell adhesion and the uniform distribution of seed cells within the scaffolds, as well as seed cell multiplication, migration and growth. Thus, the scaffolds of umbilical cord decellularized Wharton jelly in the invention can be widely applied in the tissue engineering field such ascartilage, bone, skin and nerve, with a favorable clinical application prospect.

Description

technical field [0001] The invention belongs to the technical field of medical tissue engineering, and relates to a method for extracting extracellular matrix (ECM) for tissue engineering and preparing a scaffold. Background technique [0002] The ability of cartilage tissue to repair itself is very limited, and tissue engineering technology provides a promising method for repairing cartilage defects. The main content includes seed cells, scaffold materials, cytokines and culture environment, among which, using good methods and techniques to construct ideal tissue engineering scaffolds is the key to successfully repairing articular cartilage defects. [0003] Scaffold materials currently used in cartilage tissue engineering include natural and synthetic. Synthetic scaffold materials are mainly organic synthetic materials, mainly polymers such as polylactic acid (PLA), polyglycolic acid (PGA), polyglycolic acid-lactic acid (PLGA), and polyethylene oxide (Pluronic F-127). Th...

Claims

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Application Information

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IPC IPC(8): A61L27/36
Inventor 卢世璧黄靖香刘舒云袁玫郭全义张莉彭江赵斌眭翔许文静
Owner 卢世璧
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