Preparation method and application of silicon rubber/collagen-based porous skin scaffold material

A technology of scaffold material and silicone rubber, which is applied in the field of composite porous scaffold and its preparation, can solve the problems of difficult to control the pore size, unfavorable enzymatic stability of materials, and inappropriate pore size, so as to improve hydrophilicity and improve Biocompatibility, effect of securing material stability

Active Publication Date: 2011-04-27
JIANGXI LYUTAI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the structural bionic simulation, it still focuses on the separate construction of the dermis and epidermis, and then through the method of mechanical covering, the two are bonded together. The stability on the surface needs to be further verified; at the same time, the method of cross-linking the collagen solution and then freeze-drying to ...

Method used

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  • Preparation method and application of silicon rubber/collagen-based porous skin scaffold material
  • Preparation method and application of silicon rubber/collagen-based porous skin scaffold material
  • Preparation method and application of silicon rubber/collagen-based porous skin scaffold material

Examples

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Embodiment 1

[0030] Example 1: In this example, ammonia plasma modified silicone rubber is selected, and the collagen solution is directly coated, and the dehydrating agent is a mixture of EDC / NHS. The specific steps are as follows:

[0031] The first step, plasma modification of the surface of silicone rubber: place the silicone rubber film in the plasma surface treatment instrument, open the ammonia gas valve, start the power at 30 w, and treat for 2 min, see the attached figure 1, the surface hydrophilicity of the modified silicone rubber is improved;

[0032] The second step, the coating of collagen-based biomacromolecules: Prepare 0.6% collagen solution and coat it on the surface of the treated silicone rubber film, use a freeze dryer to extract the solvent (treatment time is 10h), see attached figure 2 , the macroporous structure of the collagen layer can be seen clearly from the scanning electron microscope;

[0033] The third step, the cross-linking of the collagen layer: put the...

Embodiment 2

[0035] Embodiment 2: the present embodiment selects oxygen plasma modified silicone rubber, and directly coats the mixed solution of collagen and chitosan, and dehydrating agent selects the mixture of EDC / NHS, and concrete steps are as follows:

[0036] In the first step, the silicone rubber film is subjected to plasma treatment: place the silicone film in a plasma surface treatment instrument, open the oxygen valve, start the power at 30w, and treat for 10 minutes;

[0037] The second step, the coating of collagen-based biomacromolecules: prepare a 1.0% mixed solution of collagen and chitosan (the mass ratio of collagen / chitosan is 6:4), and coat it on the treated silicon On the surface of the rubber film, use a freeze dryer to extract the solvent (treatment time is 12 hours);

[0038] The third step, the cross-linking of the collagen layer: put the lyophilized silicone rubber / collagen-based porous scaffold into ethanol / H containing 100mM EDC / NHS (molar ratio 1 / 1) 2 O volume...

Embodiment 3

[0040] Embodiment 3: In this embodiment, hydrazine gas plasma modified silicone rubber is selected, and the collagen solution is directly coated, and the dehydrating agent is DMTMM, and the specific steps are as follows:

[0041] In the first step, the silicone rubber film is subjected to plasma treatment: the obtained silicone film is placed in a plasma surface treatment instrument, the hydrazine gas valve is opened, the starting power is 60w, and the treatment is 6 minutes;

[0042] The second part, the coating of collagen-based biomacromolecules: prepare 1.0% collagen solution, and coat it on the surface of the treated silicone rubber film, use a freeze dryer to extract the solvent, (treatment time is 12h);

[0043] The third step, cross-linking of the collagen layer: put the lyophilized silicone rubber / collagen-based porous scaffold into ethanol / HO containing 100mM DMTMM 2 O volume ratio is in the mixed solvent of 7 / 3, after reacting at room temperature for 6 hours, soak a...

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Abstract

The invention relates to a preparation method and application of a silicon rubber/collagen-based porous skin scaffold material. The preparation method comprises the following steps of: (1) putting an organic silicon film into a plasma treatment instrument, and introducing a polar group into the surface of silicon rubber; (2) preparing collagen solution or mixed solution of collagen and other biomacromolecules at certain concentration, coating the solution on the surface of a silicon rubber film obtained in the step (1), and extracting a solvent by using a freeze dryer; (3) soaking a silicon rubber/collagen-based composite scaffold obtained in the step (2) in a dehydrating agent-containing mixed solvent of ethanol and water, reacting for 6 to 12 hours at room temperature, and soaking and washing by using a large amount of deionized water; and (4) putting a scaffold obtained in the step (3) into the freeze dryer again to extract absorbed water. The silicon rubber/collagen-based composite porous material prepared by the method has high biocompatibility and appropriate pore diameter, is stable, and can be used for a skin scaffold, or a temporary dressing favorable for repairing wound parts.

Description

technical field [0001] The invention relates to the field of porous support materials, in particular to a composite material porous support with organosilicon and collagen as a matrix, a preparation method and medical application thereof. Background technique [0002] The skin is the natural barrier of our human beings, and plays the roles of protection, sensation, temperature regulation, secretion, excretion, absorption and immunity in the human body. When the skin is damaged by severe burns, wounds, and diabetic refractory skin ulcers, the loss of tissue fluids and body fluids will cause imbalances in water, electrolytes, and acid-base balance, affecting the normal absorption and metabolic balance of the human body. At the same time, it is very easy to cause wound infection, Shock, sepsis, etc. So far, autologous skin grafting is still the most effective method for clinical treatment of full-thickness skin defects. However, there are often problems such as insufficient s...

Claims

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Application Information

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IPC IPC(8): A61L27/60A61L15/30A61L15/32A61L15/42
Inventor 聂华荣沈星星陈义旺周志华胡葵葵
Owner JIANGXI LYUTAI TECH
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