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Hydroxyapatite-based biological composite scaffold and tissue engineered bone

A hydroxyapatite and composite scaffold technology, which is applied in medical science, prosthesis, etc., can solve the problem that the porosity and pore size distribution of composite materials cannot be controlled, the composite materials cannot be directly used for bone tissue repair, and the viscosity of hydroxymethyl chitosan It is easy to control the degradation rate and pore size distribution, the distribution is ideal, and the mechanical properties are good.

Inactive Publication Date: 2012-01-04
JINING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But it has the following defects: hydroxymethyl chitosan has high viscosity and is not easy to degrade
However, it has the following defects: no pore-forming agent or other effective pore-forming methods are used in the preparation process, so the porosity and pore size distribution of the obtained composite material cannot be controlled; in addition, the patent does not add cells and growth factors, and the obtained composite material cannot be directly Applied to bone tissue repair

Method used

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  • Hydroxyapatite-based biological composite scaffold and tissue engineered bone
  • Hydroxyapatite-based biological composite scaffold and tissue engineered bone
  • Hydroxyapatite-based biological composite scaffold and tissue engineered bone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of hydroxyapatite-based biocomposite scaffold

[0031] Proceed as follows:

[0032] (1) remove the cocoons of silkworm cocoons, clean them, place them in 0.02mol / L sodium carbonate solution and boil them for 30 minutes according to the bath ratio of 10:1, and then wash them with deionized water to remove sericin, which has sensitization reactions to human tissues, get silk fibroin

[0033] (2) According to HA:SF:CS=8:1:1 in the final product, weigh the above degummed silk fibroin and dissolve it in calcium chloride-ethanol-water ternary solution, stir at 80°C for 30min, until completely Dissolve to obtain silk fibroin solution, which is placed in a three-necked flask for subsequent use; wherein, in the ternary solution, the molar ratio of calcium chloride: ethanol: water is 1: 2: 8;

[0034] (3) Taking calcium chloride as the calcium source, according to the Ca / P molar ratio is 1.67 configuration concentration is the diammonium hydrogen phosphate...

Embodiment 2

[0041] Example 2 Preparation of hydroxyapatite-based biocomposite scaffold

[0042] Example 1 was repeated with the HA:SF:CS ratio changed to 7:2:1.

Embodiment 3

[0043] Example 3 Preparation of hydroxyapatite-based biocomposite scaffold

[0044] Repeat Example 1, wherein (hydroxyapatite+silk fibroin+chitosan): the mass ratio of sodium chloride is changed to 1: 1.5.

[0045] The obtained hydroxyapatite / silk fibroin-chitosan composite scaffold of above-mentioned three embodiments carries out following detection:

[0046] XRD and FT-IR detection confirmed that the composite scaffold is composed of three phases of hydroxyapatite, silk fibroin and chitosan, such as figure 1 shown;

[0047] The analysis by JEM-100Cx II SEM shows that the mesopores in the composite scaffold are connected with each other, the porosity exceeds 70%, and the average pore diameter is between 150 and 200 μm ( figure 2 );

[0048] Tested by Shenzhen Sansi universal material testing machine: the compressive strength of composite scaffolds exceeds 150MPa, which meets the requirements of bone scaffold composite materials.

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Abstract

The invention discloses a hydroxyapatite-based biological composite scaffold composed of hydroxyapatite, silk fibroin and chitosan, wherein a mass ratio of the components is represented by that: HA:SF:CS=(60-80):(10-20):(10-20). The composite scaffold is in a porous shape, wherein the porosity is 70 to 90%, and the pore size is 150 to 200mun. The pores are basically round, and are communicated with each other. The invention also discloses a tissue engineered bone constructed by using the hydroxyapatite-based biological composite scaffold. According to the invention, BMSCs transfected with VEGF gene is implanted into the hydroxyapatite-based biological composite scaffold, such that the tissue engineered bone is formed. According to the invention, hydroxyapatite, silk fibroin and chitosanare are adopted as raw materials, and a porogen-leaching technology is combined with a vacuum drying technology, such that the hydroxyapatite-based biological composite scaffold material is prepared. A degradation rate of the scaffold is regulated through the regulation of the proportions of the components. The pore size distribution of the composite material is controlled through the regulations of the radiuses and the addition amount of sodium chloride particles.

Description

technical field [0001] The invention relates to a hydroxyapatite-based biocomposite scaffold, a preparation method thereof, and a tissue engineered bone constructed therefrom, belonging to the field of biomedical materials. Background technique [0002] Bone defects caused by trauma, infection, tumor and congenital diseases in life activities are very common, especially the incidence of bone defects caused by limb trauma is as high as 10%. The treatment and repair of bone defects is a common difficult problem in orthopedics one of the symptoms. Studies have shown that the diameter of the long tubular bone diaphysis determines the length of the bone defect that can heal itself. When the length of the bone defect exceeds 1.5 to 2.5 times its diameter, it is difficult to heal itself, and bone graft materials are required for repair. However, currently clinically used bone repair materials have their own advantages and disadvantages, and cannot meet the requirements of ideal ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/54A61L27/40A61L27/22A61L27/20A61L27/12
Inventor 卢志华赵冬梅
Owner JINING UNIV
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