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Composite biological patch based on pig small intestinal submucosa and preparation method and application thereof

A submucosa and biological patch technology, applied in the field of medical materials, can solve the problems of narrow scar repair and excessive proliferation of fibroblasts, and achieve the effects of promoting adhesion on materials, prolonging life, and good cell compatibility

Inactive Publication Date: 2017-10-10
苏州期佰生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the simple SIS material is implanted in the body, it is very easy to receive host cells, and the trauma reaction caused by the implantation operation often leads to excessive proliferation of fibroblasts, and finally forms a stricture with scar repair.

Method used

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  • Composite biological patch based on pig small intestinal submucosa and preparation method and application thereof
  • Composite biological patch based on pig small intestinal submucosa and preparation method and application thereof
  • Composite biological patch based on pig small intestinal submucosa and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Clean the fresh porcine small intestine, remove the serosa layer and muscular layer by mechanical scraping, then soak the remaining small intestinal submucosa in a solution containing 0.1% peracetic acid and 10% ethanol for 1 hour, and use deionized After washing with water, soak in methanol and chloroform mixture for 2 hours, then soak in hypertonic solution for 2 hours, detergent solution for 2 hours, hypotonic solution for 2 hours, and detergent solution for 2 hours. Soak the prepared decellularized porcine small intestinal submucosa in 1mol / L NaOH solution for 1 min; rinse carefully with deionized water, freeze-dry, and sterilize with gamma rays.

[0027] The material components of the hypertonic solution described in the above operation are: 1L hypertonic solution contains 58.44g sodium chloride, 6.06g Tris, 2.92g EDTA, 1L purified water; the material components of the hypotonic solution: 1L hypotonic solution contains 1.21gTris, 1.46g EDTA, 1L purified water; Dete...

Embodiment 2

[0031] Clean the fresh porcine small intestine, remove the serosa layer and muscular layer by mechanical scraping, then soak the remaining small intestinal submucosa in a solution containing 0.5% peracetic acid and 10% ethanol for 2 hours, and use deionized After washing with water, soak in methanol and chloroform mixture for 2 hours, then soak in hypertonic solution for 2 hours, detergent solution for 2 hours, hypotonic solution for 2 hours, and detergent solution for 2 hours. Soak the prepared decellularized porcine small intestinal submucosa in 0.5mol / L NaOH solution for 5 minutes; rinse carefully with deionized water, freeze-dry, and sterilize with gamma rays.

[0032] The material components of the hypertonic solution described in the above operation are: 1L hypertonic solution contains 58.44g, sodium chloride, 6.06g Tris, 2.92g EDTA, 1L purified water; the material components of the hypotonic solution: 1L hypotonic solution contains 1.21gTris , 1.46g EDTA, 1L purified wa...

Embodiment 3

[0036] Clean the fresh porcine small intestine, remove the serosa layer and muscular layer by mechanical scraping, then soak the remaining small intestinal submucosa in a solution containing 0.5% peracetic acid and 5% ethanol for 1 hour, and use deionized After washing with water, soak in methanol and chloroform mixture for 4 hours, then soak in hypertonic solution for 2 hours, detergent solution for 2 hours, hypotonic solution for 2 hours, and detergent solution for 2 hours. Soak the prepared decellularized porcine small intestinal submucosa in 0.5 mol / L NaOH solution for 5 minutes; rinse carefully with deionized water, freeze-dry, and sterilize with gamma rays.

[0037] The material components of the hypertonic solution described in the above operation are: 1L hypertonic solution contains 58.44g sodium chloride, 6.06g Tris, 2.92g EDTA, 1L purified water; the material components of the hypotonic solution: 1L hypotonic solution contains 1.21gTris, 1.46g EDTA, 1L purified water...

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Abstract

The invention discloses a composite biological patch based on pig small intestinal submucosa and a preparation method and application thereof. The composite biological patch is characterized in that the accellular pig small intestinal submucosa is compounded with an additive so as to obtain the composite biological patch based on the pig small intestinal submucos; the additive is selected from one or two of growth factors and medicines. The composite biological patch based on the pig small intestinal submucos has the advantages that the composite biological patch is a good biological stent material, the structure component and the space three-dimensional structure are very similar, the good biological compatibility is presented in the clinical experiment, the healing of wounds is promoted, and various tissues are regenerated and remolded; the biological stent material has effective restoration effect on the healing of skin wounds, and has important meaning on the regeneration of the skins.

Description

technical field [0001] The invention relates to a medical material, in particular to a composite biological patch based on porcine small intestinal submucosa, a preparation method and application thereof. Background technique [0002] The skin is the largest organ of the human body and has important physical, chemical and biological barrier functions. The repair of full-thickness skin defect wounds is a basic problem in the field of surgery. Full-thickness skin defects not only require timely and permanent wound coverage but also maximize It has become an important goal of treatment to restore the function and appearance to the maximum, repair the defective skin as soon as possible and restore the function and appearance as much as possible; in the repair and function recovery of skin tissue, vascularization has become a bottleneck problem. The pig small intestine is mainly composed of 4 layers, which are the mucosa layer, submucosa layer, muscular layer and serosa layer fro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/54A61L27/36
Inventor 尹华清方帆
Owner 苏州期佰生物技术有限公司
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