Neural restoration promoting material as well as preparation method and application thereof

A nerve repair and nerve growth factor technology, applied in the field of nerve repair materials, can solve the problems of unsatisfactory drug release stability, difficult control of release speed and activity, and potential safety hazards, so as to achieve safe use and stable effect , low-cost effect

Active Publication Date: 2013-01-09
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of directly adding growth factors will release the growth factors in a short period of time, the release rate and activity are difficult to control, the effectiveness is poor, and there are potential safety hazards
Sustained-release drug delivery technology can control the release rate of growth factors in the body, but most of the existing sustained-release materials are polymer synthetic materials, which are expensive, and the stability of drug release is not satisfactory, and may cause damage to the body due to metabolic problems. bring side effects

Method used

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  • Neural restoration promoting material as well as preparation method and application thereof
  • Neural restoration promoting material as well as preparation method and application thereof
  • Neural restoration promoting material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 Preparation and detection of nerve-promoting repair materials of the present invention

[0044] 1. Isolation, purification, culture and detection of Schwann cell SCs

[0045] 1. Isolation, purification and culture of Schwann cell SCs

[0046] (1) Material collection: 20 SD suckling mice aged 5-7 days were taken and killed by necking. Under sterile conditions, the bilateral sciatic nerves were taken, and the epineurium tissue was peeled off as soon as possible under a dissecting microscope with microscopic instruments.

[0047] (2) Digestion: Digest with 0.25% trypsin and 0.2% type II collagenase for 10 minutes respectively, centrifuge at 1200rpm for 5 minutes, and inoculate once for 20 minutes after differential attachment.

[0048] (3) Purification: After 24 hours, replace with 20μl / ml G418 (200μg / mL) or 2×10 -6 M~1×10 -5 M cytarabine was cultured in 10% FBS H-DMEM medium. After two days of G418 or cytarabine treatment, the H-DMEM medium containing 10%...

Embodiment 2

[0090] Example 2 Preparation and detection of nerve-promoting repair materials of the present invention

[0091] 1. Subculture, cryopreservation and detection of RSC96 cells

[0092] 1. Subculture of RSC96 cells

[0093] (1) Subculture: RSC96 cells were purchased from the Cell Bank of the Chinese Academy of Sciences. Such as Figure 7 When the RSC96 cells grow to about 80% confluent, aspirate the medium, add 0.5ml of PBS solution containing 0.25% trypsin and 0.1% EDTA to digest, shake the digestion solution for about 2-5 minutes, the cells shrink and change under the microscope After the circle or a few fall off, gently shake the bottom of the bottle to make all the cells fall off, add 5ml of H-DMEM medium containing 10% FBS, blow gently to make the cells basically form a single suspension, and divide them into 4 cell culture flasks. Supplement H-DMEM medium containing 10% FBS to about 5ml, place in 5% CO 2 cultured in a constant temperature incubator at 37°C.

[0094] (2...

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Abstract

The invention discloses a material for promoting neural restoration. The material comprises a small intestinal submucosa and a nerve growth factor of which the content is 1 to 500pg / cm<2>. The invention also discloses a preparation method and application of the neural restoration promoting material. According to the material for promoting neural restoration, the nerve growth factor can be slowly released at (42.01+ / -1.43)-(82.43+ / -1.89)pg / cm<2> each day, so that the shortcoming caused by directly adding the growth factor can be overcome; and the preparation method is simple, the cost is low, and the material has good application prospect.

Description

technical field [0001] The invention relates to a material for promoting nerve repair and belongs to the field of biological materials. Background technique [0002] At present, the ideal tissue / organ repair is: to form the integrity of the structure, that is, to repair the appearance, and to restore the function of the tissue / organ, to form a functionally integrated repair, including vascular reconstruction, nerve reconstruction, etc. [0003] Small intestinal submucosa (SIS) is a natural extracellular matrix biomaterial, usually prepared from porcine small intestine. SIS mainly contains collagen, amino polysaccharide, glycoprotein and other components, and contains a variety of growth factors, which play an important role in tissue repair and reconstruction and cell growth, such as fibroblast growth factor, transforming growth factor and vascular endothelial growth factor, etc. It can mediate cell signal transduction by interacting with various receptors on the cell surfa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/54A61K35/30A61K38/18A61K47/46A61P3/02
Inventor 解慧琪刘宏银罗静聪杨志明
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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