Tissue patch and preparation method thereof

A patch and tissue technology, applied in the field of tissue patch and its preparation, can solve problems such as the application of such products and literature reports, and achieve the effects of convenient clinical suture and fixation, promotion of growth and proliferation, and satisfaction of wound repair

Active Publication Date: 2009-02-18
SHAANXI RUISHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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Examples

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example 1

[0018] 1. Preparation of acellular amniotic membrane: Amniotic membrane was taken from the placenta of healthy puerpera delivered by cesarean section. The amniotic membrane was peeled off under aseptic operation, rinsed with normal saline, and the amniotic membrane and chorion were separated to obtain amniotic membrane, which was soaked in 1.25g / L pancreas. Protease and 0.2g / L edetate disodium mixed digestion solution, shaking and digesting at 37°C for 1 hour, rinsed with PBS, and then put it into DNase solution containing 30U / ml, in Soak at 37°C for 30 minutes, rinse with PBS and set aside;

[0019] 2. Preparation of decellularized small intestinal submucosa: Rinse the porcine small intestine with water, cut into pieces, soak and disinfect in an aqueous solution containing 0.2% peracetic acid and 10% ethanol for 2 hours, rinse with PBS; scrape off the small intestinal mucosa , muscular layer and serosa, rinsed with PBS; put them in 1M NaOH solution, soak for 10 minutes at 4°C...

example 2

[0023] 1. Preparation of decellularized amniotic membrane: Amniotic membrane is taken from the placenta of healthy puerpera, the amniotic membrane is peeled off under aseptic operation, rinsed with normal saline, the amniotic membrane and chorion are separated to obtain amniotic membrane, which is soaked in a solution containing 2.5g / L trypsin and 0.4g / L mixed digestion solution of disodium ethylenediaminetetraacetate, digested by shaking at 37°C for 1.5 hours, rinsed with PBS, then put it into the DNase solution containing 40U / ml, soaked at 37°C for 30 Minutes, rinse with PBS and set aside;

[0024] 2. Preparation of the submucosa of the decellularized small intestine: Rinse the bovine small intestine with water, cut into pieces, soak and disinfect in an aqueous solution containing 0.4% peracetic acid and 5% ethanol for 4 hours, rinse with PBS; scrape off the small intestinal mucosa , muscular layer and serosa were rinsed with PBS to obtain the small intestinal submucosa; th...

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Abstract

The invention provides a tissue patch and a preparation method thereof. The tissue patch uses an acellular small intestinal submucosa as an inner layer, the two sides of the tissue patch are covered with acellular amniotic membranes; the prepared tissue patch isolates from immunotoxicity of the acellular small intestinal submucosa, and the acellular small intestinal submucosa compensates for the deficiencies of a mechanical strength of the acellular amniotic membrane. Compared with the prior art, the tissue patch has higher biological activity and biocompatibility, no obvious immune rejection and no toxic effect on cells; and the preparation method has the advantages of unlimited raw materials source, cell-free residues, no ethical issues and capability of effectively inactivating virus. The prepared product is applicable to serving as a tissue reinforcement to repair a shortcoming or a defect of a strength of a body soft tissue; the prepared product is used as a tissue filling material and is applicable to the repair of facial depression deformity.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering biomaterials, and in particular relates to a tissue patch and a preparation method thereof. Background technique [0002] The ideal method for wound coverage is autologous skin grafting, but since autologous skin sources are often insufficient, skin substitutes are required. The tissue patch made of extracellular matrix has biocompatibility unmatched by other materials, and is currently the most promising wound covering, mainly including allogeneic and heterogeneous skin, amniotic membrane, animal peritoneum, collagen membrane and other materials . [0003] The extracellular matrix (ECM) provides a suitable place for the survival and activity of cells. It is not only the mechanical support tissue of cells, but also the place for nutrient supply and immune response. It participates in the regulation of embryonic development and determines the adhesion and Migration plays an important r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/26
Inventor 王爱军
Owner SHAANXI RUISHENG BIOTECH
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