Tissue patch and preparation method thereof

Abstract

The invention provides a tissue patch and a preparation method thereof. The tissue patch uses an acellular small intestinal submucosa as an inner layer, the two sides of the tissue patch are covered with acellular amniotic membranes; the prepared tissue patch isolates from immunotoxicity of the acellular small intestinal submucosa, and the acellular small intestinal submucosa compensates for the deficiencies of a mechanical strength of the acellular amniotic membrane. Compared with the prior art, the tissue patch has higher biological activity and biocompatibility, no obvious immune rejection and no toxic effect on cells; and the preparation method has the advantages of unlimited raw materials source, cell-free residues, no ethical issues and capability of effectively inactivating virus. The prepared product is applicable to serving as a tissue reinforcement to repair a shortcoming or a defect of a strength of a body soft tissue; the prepared product is used as a tissue filling material and is applicable to the repair of facial depression deformity.

Description

technical field

[0001] The invention belongs to the technical field of tissue engineering biomaterials, and in particular relates to a tissue patch and a preparation method thereof. Background technique

[0002] The ideal method for wound coverage is autologous skin grafting, but since autologous skin sources are often insufficient, skin substitutes are required. The tissue patch made of extracellular matrix has biocompatibility unmatched by other materials, and is currently the most promising wound covering, mainly including allogeneic and heterogeneous skin, amniotic membrane, animal peritoneum, collagen membrane and other materials .

[0003] The extracellular matrix (ECM) provides a suitable place for the survival and activity of cells. It is not only the mechanical support tissue of cells, but also the place for nutrient supply and immune response. It participates in the regulation of embryonic development and determines the adhesion and Migration plays an important r...