Novel composite biological dura mater

A biomembrane and dura mater technology, applied in the field of new composite biological dura mater, can solve problems such as difficult to achieve functional zero biomimetic regenerative repair effect, achieve the promotion of fibroblast activity, satisfactory effect, and reduce scar tissue and nerve adhesion Effect

Inactive Publication Date: 2014-10-29
王伟
View PDF0 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, both SIS and collagen have been successfully used in engineering scaffolds in the field of regenerative medicine, wound repair, tissue regeneration, etc., but limited by their respective structures,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Preparation of Type I Collagen Fiber Dura

[0030] 1) Collagen extraction, reconstitution and lyophilization

[0031] 1.1 Raw material thawing:

[0032] The collagen is taken out from the freezer, and the usage amount of the batch is calculated according to the water content, placed in a sterilized plate, and used after thawing at room temperature.

[0033] 1.2 Preparation calculation method:

[0034] 0.05M, 600ml glacial acetic acid solution containing 3-5g dry collagen

[0035] 1.3 Preparation of type I collagen solution. Weigh 9.1-15.2g of the thawed collagen according to the water content and put it into a stirring tank, add 600-800ml of 0.05M glacial acetic acid, and stir for 1-2 hours.

[0036] 1.4 Freeze-drying: Put the solution in 1.3 into a plate, pour in different thicknesses of type I collagen solution according to clinical needs, and perform freeze-drying according to the following procedures:

[0037] step number

control process

Laye...

Embodiment 2

[0044] Example 2 Porosity Measurement

[0045]The method of Example 1 was adopted to prepare 4 kinds of products with different irradiation intensities, and the pore size in the structure was measured by electron microscope to be 345um, 220um; 227um, 183um, wherein the irradiation dose was 30-40kGy group I type collagen fiber membrane pore size Most suitable for the growth of fibroblasts.

Embodiment 3

[0046] The mensuration of embodiment 3 collagenase enzymolysis time

[0047] Using a self-made hole puncher, 4 kinds of dura mater substitute materials with different irradiation times were made into 10 disks each with a diameter of 5 mm, and then the small disks, enzymatic hydrolysis buffer and collagenase solution with an enzyme activity unit of 357 U / mL were added. In a small test tube, the time required for enzymatic hydrolysis at a constant temperature of 37°C to the decomposition of the small disc is the enzymatic hydrolysis time (min). The measured enzymatic hydrolysis time is: 25.3min, 23.6min, 27.6min, 51.1min. When the irradiation dose is 25-40KGy, the enzymatic hydrolysis time of type I collagen fiber membrane has little difference, which proves its effect on collagen The degree of cross-linking has little effect.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a novel composite biological dura mater. According to the dura mater, a dura mater substitute is prepared by compounding SIS (small intestinal submucosa) and collagen sponge, is relatively close to a natural dura mater, has the characteristics of being degradable, plastic and modifiable and does not need stitching. The product is relatively substantially improved in performances, mechanical strength and safety. An innovative crosslinking manner enables the product degradation time to be consistent with newborn tissue growth time, and the product is better than clinical a same kind of products. The state of the artificial dura mater patch is innovatively designed according to the thickness difference of different parts of human dura mater, and defects of conventional clinical dura mater products are effectively solved.

Description

technical field [0001] The invention relates to a novel composite biological dura mater, in particular to a dura mater with a double-layer composite structure of collagen and SIS membrane and its application. Background technique [0002] The dura mater is an important barrier to protect brain tissue, and is of great significance for maintaining the structure and functional activities of the nervous system. Trauma or craniocerebral surgery can cause dura mater defect. If it is not repaired, the wound will not heal easily, and complications such as subcutaneous fluid, cerebrospinal fluid leakage, and intracranial infection will occur; bleeding from the scalp or muscle layer can spread to the arachnoid The lower space stimulates the brain tissue, causing fever, headache, aseptic inflammation or meningeal irritation; the direct adhesion and healing of the brain tissue and the scalp increases the chance of epilepsy. Surgery to repair the dura mater and seal the subdural space c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L27/36A61L27/24
Inventor 王伟
Owner 王伟
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap